Microtubules are involved in maintaining the cellular polarity in pollen tubes ofNicotiana sylvestris

Joos, Ute; Aken, Jan van; Kristen, U.

doi:10.1007/bf01360732

Cited by 74 publications

(55 citation statements)

References 49 publications

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“…However, root hair growth also is directed by an endogenous polarity that is not found in pollen tubes (Bibikova et al, 1997). Similarly, root hair growth polarity is mediated by microtubules (Bibikova et al, 1999), whereas that of pollen tubes is independent of microtubules (Joos et al, 1994). Microtubule-disrupting drugs produce wavy root hairs and hairs with multiple branches at their tips (Bibikova et al, 1999;Ovecka et al, 2000).…”

Section: Rop2 Controls Tip Growth During Root Hair Developmentmentioning

confidence: 99%

The Arabidopsis Rop2 GTPase Is a Positive Regulator of Both Root Hair Initiation and Tip Growth

et al. 2002

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Root hairs provide a model system for the study of cell polarity. We examined the possibility that one or more members of the distinct plant subfamily of RHO monomeric GTPases, termed Rop, may function as molecular switches regulating root hair growth. Specific Rops are known to control polar growth in pollen tubes. Overexpressing Rop2 (Rop2 OX) resulted in a strong root hair phenotype, whereas overexpressing Rop7 appeared to inhibit root hair tip growth. Overexpressing Rops from other phylogenetic subgroups of Rop did not give a root hair phenotype. We confirmed that Rop2 was expressed throughout hair development. Rop2 OX and constitutively active GTP-bound rop2 (CA-rop2) led to additional and misplaced hairs on the cell surface as well as longer hairs. Furthermore, CA-rop2 depolarized root hair tip growth, whereas Rop2 OX resulted in hairs with multiple tips. Dominant negative GDP-bound Rop2 reduced the number of hair-forming sites and led to shorter and wavy hairs. Green fluorescent protein-Rop2 localized to the future site of hair formation well before swelling formation and to the tip throughout hair development. We conclude that the Arabidopsis Rop2 GTPase acts as a positive regulatory switch in the earliest visible stage in hair development, swelling formation, and in tip growth.

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Section: Rop2 Controls Tip Growth During Root Hair Developmentmentioning

confidence: 99%

The Arabidopsis Rop2 GTPase Is a Positive Regulator of Both Root Hair Initiation and Tip Growth

et al. 2002

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“…Microtubules are made up of tubulin and are known to interact with microtubule associated proteins (MAP) (Hussey et al, 2002b). Microtubules were proposed to be involved in the regulation of pollen tube morphogenesis and in the maintenance of internal cytoplasmic organization, but not in active pollen tube growth (Joos et al, 1994;Derksen et al, 1995). They also have been shown to regulate the position of the male germ unit (Sorri et al, 1996;Rodriguez-Garcia et al, 2003).…”

Section: B a D Cmentioning

confidence: 99%

Gametophyte interaction and sexual reproduction: how plants make a zygote

Boavida¹,

Vieira²,

Becker³

et al. 2005

Int. J. Dev. Biol.

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The evolutionary success of higher plants relies on a very short gametophytic phase, which underlies the sexual reproduction cycle. Sexual plant reproduction takes place in special organs of the flower: pollen, the male gametophyte, is released from the anthers and then adheres, grows and interacts along various tissues of the female organs, collectively known as the pistil. Finally, it fertilizes the female gametophyte, the embryo sac. Pollen is released as bi or tricellular, highly de-hydrated and presumably containing all the biochemical components and transcripts to germinate. Upon hydration on the female tissues, it develops a cytoplasmic extension, the pollen tube, which is one of the fastest growing cells in nature. Pollen is completely "ready-to-go", but despite this seemingly simple reaction, very complex interactions take place with the female tissues. In higher animals, genetic mechanisms for sex determination establish striking developmental differences between males and females. In contrast, most higher plant species develop both male and female structures within the same flower, allowing self-fertilization. Outcrossing is ensured by self-incompatibility mechanisms, which evolved under precise genetic control, controlling self-recognition and cell-to-cell interaction. Equally important is pollen selection along the female tissues, where interactions between different cell types with inherent signalling properties correspond to check-points to ensure fertilization. Last but not least, pollen-pistil interaction occurs in a way that enables the correct targeting of the pollen tubes to the receptive ovules. In this review, we cover the basic mechanisms underlying sexual plant reproduction, from the structural and cellular determinants, to the most recent genetic advances.

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“…This is reflected in the polarized cytological organization: the vegetative nucleus, the generative cell, and larger organelles are located in the distal region of the tube, whereas the apical dome contains mainly small vesicles and endoplasmic reticulum (Pierson and Cresti, 1992). Actin bundles and microtubules extend axially from the pollen grain along the length of the pollen tube to the subapical region, where the overall direction of cytoplasmic flow reverses from anterograde to retrograde (Derksen et al, 1985;Lancelle and Hepler, 1991;Joos et al, 1994;Astrom et al, 1995). The apical dome contains sparse anastomosing actin filaments (Lancelle and Hepler, 1991;Miller et al, 1996;Kost et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate

et al. 2002

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Microtubules are involved in maintaining the cellular polarity in pollen tubes ofNicotiana sylvestris

Cited by 74 publications

References 49 publications

The Arabidopsis Rop2 GTPase Is a Positive Regulator of Both Root Hair Initiation and Tip Growth

The Arabidopsis Rop2 GTPase Is a Positive Regulator of Both Root Hair Initiation and Tip Growth

Gametophyte interaction and sexual reproduction: how plants make a zygote

Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate

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