Microvascular circulation at cool, normal and warm temperatures in rat leg muscles examined by histochemistry using Lycopersicon esculentum lectin

Maeda, Hisashi; Kurose, Tomoyuki; Nosaka, Shinnosuke; Kawamata, Seiichi

doi:10.1016/j.acthis.2014.05.006

Cited by 4 publications

(9 citation statements)

References 27 publications

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“…In the present study, the proportion of open and functioning capillaries was semi-automatically measured using one section after double immunostaining. The results for skeletal muscles were generally in agreement with previous studies (Kayar and Banchero 1985 ; Kindig et al 2002 ; Kano et al 2005 ; Maeda et al 2014 ). The proportions of open and functioning capillaries of skeletal muscles, pancreas and small intestine were high and not significantly different, in spite of their structural and functional differences.…”

Section: Discussionsupporting

confidence: 92%

“…The proportions of open and functioning capillaries of skeletal muscles, pancreas and small intestine were high and not significantly different, in spite of their structural and functional differences. Our finding seems to support the notion that microvascular blood flow in various tissues and organs is mainly controlled by regulating the blood flow of each capillary, while the proportion of open and functioning capillaries remains relatively constant (Kindig et al 2002 ; Maeda et al 2014 ).…”

Section: Discussionsupporting

confidence: 89%

“…On the other hand, Poole et al ( 2013 ) stated that the majority of capillaries support blood flow at rest and are not available to be recruited. The proportion of open and functioning capillaries has been repeatedly measured in skeletal muscles (Renkin et al 1981 ; Kayar and Banchero 1985 ; Kindig et al 2002 ; Kano et al 2005 ; Maeda et al 2014 ) and remains controversial (Poole 2014 ; Barrett et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

“…Concerning microvascular studies, Lycopersicon esculentum lectin is well known to bind endothelial cells and has been employed in combination with PECAM-1 immunostaining (Hashizume et al 2000 ; Morikawa et al 2002 ; Inai et al 2004 ; Mazzetti et al 2004 ; Nakamura-Ishizu et al 2008 ; Maeda et al 2014 ; Robertson et al 2015 ). In this method, the plasma flow results in lectin binding to endothelial cells, even without moving red blood cells.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, two serial cross sections were used to measure the proportion of open and functioning capillaries in rat leg muscles (Maeda et al 2014 ). In paired cross sections of skeletal muscles, the number, location and direction of blood vessels are almost the same since most blood vessels run along the muscle fiber’s long axis.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

2015

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To study the microvascular circulation, we examined the proportion of open and functioning capillaries in the leg muscles, pancreas and small intestine of anesthetized rats. Fluorescein isothiocyanate (FITC)-labeled Lycopersicon esculentum lectin was injected into the heart and allowed to circulate for 3 min to label open and functioning capillaries. Specimens were removed, frozen, sectioned and double-immunostained. Using one section, open and functioning capillaries were detected by immunostaining for this lectin bound to endothelial cells, while all capillaries were visualized by immunostaining for platelet endothelial cell adhesion molecule-1 (PECAM-1 or CD31). These capillaries were semi-automatically detected and counted by fluorescence microscopy. The percentages of open and functioning capillaries were as follows: the soleus muscle, 93.0 ± 5.5%; superficial zone of the gastrocnemius muscle, 90.8 ± 6.2%; deep zone of the gastrocnemius muscle, 95.6 ± 4.0%; the plantaris muscle, 94.1 ± 2.7%; the pancreas, 86.3 ± 11.7%; and the small intestine, 91.1 ± 4.9% (n = 8, each). There was no significant difference among these data by the Kruskal–Wallis test. This study clearly demonstrated that the proportions of open and functioning capillaries are high and similar among the leg muscles, pancreas and small intestine in spite of their structural and functional differences. This finding agrees with previous studies and supports the notion that the microvascular circulation is mainly controlled by changing of the blood flow in each capillary rather than changing the proportion of open and functioning capillaries.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

2015

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Increased contribution of KCa channels to muscle contraction induced vascular and blood flow responses in sedentary and exercise trained ZFDM rats

Sonobe

Tsuchimochi

Maeda

et al. 2022

The Journal of Physiology

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In resistance arteries, endothelium‐dependent hyperpolarization (EDH)‐mediated vasodilatation is depressed in diabetes. We hypothesized that downregulation of KCa channel derived EDH reduces exercise‐induced vasodilatation and blood flow redistribution in diabetes. To test this hypothesis, we evaluated vascular function in response to hindlimb muscle contraction, and the contribution of KCa channels in anaesthetised ZFDM, metabolic disease rats with type 2 diabetes. We also tested whether exercise training ameliorated the vascular response. Using in vivo microangiography, the hindlimb vasculature was visualized before and after rhythmic muscle contraction (0.5 s tetanus every 3 s, 20 times) evoked by sciatic nerve stimulation (40 Hz). Femoral blood flow of the contracting hindlimb was simultaneously measured by an ultrasonic flowmeter. The contribution of KCa channels was investigated in the presence and absence of apamin and charybdotoxin. We found that vascular and blood flow responses to muscle contraction were significantly impaired at the level of small artery segments in ZFDM fa/fa rats compared to its lean control fa/+ rats. The contribution of KCa channels was also smaller in fa/fa than in fa/+ rats. Low‐intensity exercise training for 12 weeks in fa/fa rats demonstrated minor changes in the vascular and blood flow response to muscle contraction. However, the KCa‐derived component in the response to muscle contraction was much greater in exercise trained than in sedentary fa/fa rats. These data suggest that exercise training increases the contribution of KCa channels among endothelium‐dependent vasodilatory mechanisms to maintain vascular and blood flow responses to muscle contraction in this metabolic disease rat model. Key points Microvascular dysfunction in type 2 diabetes impairs blood flow redistribution during exercise and limits the performance of skeletal muscle and may cause early fatigability. Endothelium‐dependent hyperpolarization (EDH), which mediates vasodilatation in resistance arteries, is known to be depressed in animals with diabetes. Here, we report that low‐intensity exercise training in ZFDM rats increased the KCa channel‐derived component in the vasodilator responses to muscle contraction compared to that in sedentary rats, partly as a result of the increase in KCNN3 expression. These results suggest that low‐intensity exercise training improves blood flow redistribution in contracting skeletal muscle in metabolic disease with diabetes via upregulation of EDH.

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Usefulness of cyclic thermal therapy and red blood cell scintigraphy in patients with chemotherapy-induced peripheral neuropathy

Kim

et al. 2021

Korean J Pain

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Background Pharmacological and non-pharmacological therapies have been used to treat patients with chemotherapy-induced peripheral neuropathy (CIPN). However, the effect of therapies in cancer patients has yet to be investigated comprehensively. We hypothesized that cyclic thermal therapy would improve blood flow and microcirculation and improve the symptoms driven by CIPN. Methods The criteria of assessment were blood volume in region of interest (ROI) in the images, and European Organization for Research and Treatment of Cancer–Quality of Life Questionnaire–Chemotherapy-Induced Peripheral Neuropathy 20 questionnaire scores. The blood volume was quantified by using red blood cell (RBC) scintigraphy. All patients were treated 10 times during 10 days. The thermal stimulations, between 15° and 41°, were repeatedly delivered to the patient’s hands. Results The total score of the questionnaires, the score of questions related to the upper limbs, the score of questions closely related to the upper limbs, and the score excluding the upper limbs questions was decreased. The blood volume was decreased, and the variance of blood volume was decreased. During cooling stimulation, the blood volume was decreased, and its variance was decreased. During warming stimulation, the blood volume was decreased, and its variance was decreased. Conclusions We suggest that cyclic thermal therapy is useful to alleviate CIPN symptoms by blood circulation improvement. RBC scintigraphy can provide the quantitative information on blood volume under certain conditions such as stress, as well as rest, in peripheral tissue.

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Microvascular circulation at cool, normal and warm temperatures in rat leg muscles examined by histochemistry using Lycopersicon esculentum lectin

Cited by 4 publications

References 27 publications

Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

Regulation of the microvascular circulation in the leg muscles, pancreas and small intestine in rats

Increased contribution of KCa channels to muscle contraction induced vascular and blood flow responses in sedentary and exercise trained ZFDM rats

Usefulness of cyclic thermal therapy and red blood cell scintigraphy in patients with chemotherapy-induced peripheral neuropathy

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