2014
DOI: 10.1016/j.bpj.2013.11.4502
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Microvascular Endothelial Cells Migrate Upstream and Align Against the Shear Stress Field Created by Impinging Flow

Abstract: At present, little is known about how endothelial cells respond to spatial variations in fluid shear stress such as those that occur locally during embryonic development, at heart valve leaflets, and at sites of aneurysm formation. We built an impinging flow device that exposes endothelial cells to gradients of shear stress. Using this device, we investigated the response of microvascular endothelial cells to shear-stress gradients that ranged from 0 to a peak shear stress of 9-210 dyn/cm(2). We observe that a… Show more

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Cited by 84 publications
(90 citation statements)
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“…Endothelial cells can also sense whether flow is parallel or perpendicular to the long axis of their cell body . Furthermore, accelerating gradients in shear stress elicit responses that are not seen in decelerating gradients of the same magnitude (Szymanski et al 2008;Dolan et al 2011;Ostrowski et al 2014). As such, it is clear that endothelial cells distinguish between different types of shear stress, but how they recognize and respond in a manner that shapes artery development is poorly understood.…”
Section: Dach1 In Shear Stress Responses and Arteriogenesismentioning
confidence: 99%
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“…Endothelial cells can also sense whether flow is parallel or perpendicular to the long axis of their cell body . Furthermore, accelerating gradients in shear stress elicit responses that are not seen in decelerating gradients of the same magnitude (Szymanski et al 2008;Dolan et al 2011;Ostrowski et al 2014). As such, it is clear that endothelial cells distinguish between different types of shear stress, but how they recognize and respond in a manner that shapes artery development is poorly understood.…”
Section: Dach1 In Shear Stress Responses and Arteriogenesismentioning
confidence: 99%
“…Cells were cultured using an impinging flow device that jets medium perpendicular to the culture surface, creating regions where shear stress accelerates from 0 to 35 dynes/cm 2 over ∼10 cell lengths before decelerating back to negligible flow rates 1.25 cm away from the impingement point ( Fig. 6D; Ostrowski et al 2014). Cells cultured under impinging flow only mildly decreased their DACH1 expression even at the highest shear stress magnitudes (Fig.…”
Section: Dach1 Is Down-regulated By Arterial-type Shear Stress In Vitromentioning
confidence: 99%
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“…Using this device, we found that human lymphatic microvascular endothelial cells (HLMVECs), uniquely among the cells assayed, migrated upstream, against the flow direction [35]. Here, we show that this upstream migratory phenotype requires both S1PR1 and its ligand S1P.…”
Section: Introductionmentioning
confidence: 83%
“…Previously, we developed an impinging flow chamber (IFC) to impart controlled spatial gradients in WSS to a monolayer of adherent cells in a six-well plate fashion [35][36][37]. Using this device, we found that human lymphatic microvascular endothelial cells (HLMVECs), uniquely among the cells assayed, migrated upstream, against the flow direction [35].…”
Section: Introductionmentioning
confidence: 99%