Microvasculature as studied by the microvascular corrosion casting/scanning electron microscope method. I. Endocrine and digestive system.

Blood vascular architecture of the rat cerebral hypophysis and hypothalamus. A dissection/scanning electron microscopy of vascular casts.

“…The results obtained by these authors, who mainly used light microscope methods (light microscopy of India ink-or other dye-injected and sectioned or transilluminated specimens, vital light microscopy of living tissues, and light microscopy of latex-injected and corroded samples), have confirmed that the hypophyseal portal system occurs throughout the vertebrates, though this system becomes increasingly more complicated with the evolutionary order of the species (GREEN, 1951(GREEN, , 1966HASEGAWA, 1960 ;CUMMINGS and HABEL, 1965 ;DANIEL, 1966;WINGSTRAND, 1966). Similar findings have been obtained by modern workers who generally using the vascular casting/scanning electron microscope method (MURA KAMI, 1975a, b;PAGE et al, 1976PAGE et al, , 1978PAGE and BERGLAND, 1977;LAMET-SCHWANDTNER et al, 1977 ;BERGLAND and PAGE, 1979 ;HODDE, 1981 ;PAINO et al, 1981 ;LAMETSCHWANDTNER, 1982 ;OHTANI et al, 1983 ;KIKUTA et al, 1984;MURAKAMI et al, 1985). It has been also confirmed by the vacular casting/scanning electron microscope method that, in animals with a long hypophyseal stalk (e. g., monkey and rat), the portal (or long portal) vessels are thick, though their rate of ocurrence is rather limited, while in animals with a short hypophyseal stalk (e. g., dog and guinea pig), the long portal vessels are thin and numerous (MURAKAMI,1975a, b ;PAGE et al, 1976 ;BERGLAND and PAGE, 1979).…”

“…Scanning observation of the vascular casts has clearly comfirmed in the monkey, dog, rat and some other animals this, or long portal system, as well as the so-called short portal system connecting the infundibular process and pars distalis (MURAKAMI, 1975a, b ;PAGE et al, 1976PAGE et al, , 1978PAGE and BERGLAND, 1977;LAMETSCHWANDTNER et al, 1977;PAGE, 1978, 1979;OHTANI et al, 1983;KIKUTA et al, 1984;MURAKAMI et al,' 1985). Another short or intraadenohypophyseal portal system between the pars intermedia and pars distalis has been further documented in the rat (MURAKAMI, 1975a;MURAKAMI et al, 1985).…”

A monomeric methyl and hydroxypropyl methacrylate injection medium and its utility in casting blood capillaries and liver bile canaliculi for scanning electron microscopy.

“…Scanning electron microscopy (SEM) of casted samples, now considered a standard method for studying microcirculation, has unraveled the microvascular organization of many tissues and organs which previously were only poorly visualized by conventional light or transmission electron microscopy (MURAKAMI, 1971(MURAKAMI, , 1972FUJITA and MURAKAMI, 1973;NOWELL and LOHSE, 1974;LAMETSCHWANDTNER and SIMONSBERGER, 1975;LEE and DEMPSEY,1976;PAGE et al, 1976;CANNON, 1978;MORRIS and CAMPBELL, 1978;HODDE and NOWELL, 1980;BHALLA et al, 1981;OHTANI et al, 1983;OLSON, 1983;ROGERS and CANNON, 1983). Many casting or injection media (polyester, latex, rubber, etc.…”

Intussusceptive angiogenesis: Its emergence, its characteristics, and its significance

“…Once the concept was born, we searched the literature and rapidly noticed that the tiny "holes" characteristic of intussusception in vascular casts could easily be made out in numerous publications about the vasculature of various organs and different animal species (see, e.g., Ohashi et al, 1976;Olson, 1980;Ohtani et al, 1983;Hossler and Olson, 1984;Takemori et al, 1984).…”