Microvesicles and exosomes: Opportunities for cell-derived membrane vesicles in drug delivery

Dommelen, Susan M. van; Vader, Pieter; Lakhal, Samira; Kooijmans, Sander A.A.; Solinge, Wouter W. van; Wood, Matthew; Schiffelers, Raymond M.

doi:10.1016/j.jconrel.2011.11.021

Cited by 364 publications

(286 citation statements)

References 128 publications

Supporting

Mentioning

276

Contrasting

Order By: Relevance

“…As the natural carrier, they transfer signal molecules, such as protein, DNA, mRNAs, and microRNA,18 from original cells to recipient cells to facilitate cell‐to‐cell communication 19. Compared with conventional carriers, such as liposomes, nanospheres, micelles, microemulsion, and different kinds of conjugates, exosomes afford all the desirable advantages, such as low toxicity, low immunogenicity, high stability in circulation, biocompatibility, and biological barrier permeability,20 which makes them promising carriers for efficient drug or therapeutic gene delivery.…”

Section: Introductionmentioning

confidence: 99%

Engineered Exosomes With Ischemic Myocardium‐Targeting Peptide for Targeted Therapy in Myocardial Infarction

Wang

Chen

Zhao

et al. 2018

JAHA

286

206

View full text Add to dashboard Cite

BackgroundExosomes are membranous vesicles generated by almost all cells. Recent studies demonstrated that mesenchymal stem cell–derived exosomes possessed many effects, including antiapoptosis, anti‐inflammatory effects, stimulation of angiogenesis, anticardiac remodeling, and recovery of cardiac function on cardiovascular diseases. However, targeting of exosomes to recipient cells precisely in vivo still remains a problem. Ligand fragments or homing peptides discovered by phage display and in vivo biopanning methods fused to the enriched molecules on the external part of exosomes have been exploited to improve the ability of exosomes to target specific tissues or organs carrying cognate receptors. Herein, we briefly elucidated how to improve targeting ability of exosomes to ischemic myocardium.Methods and ResultsWe used technology of molecular cloning and lentivirus packaging to engineer exosomal enriched membrane protein (Lamp2b) fused with ischemic myocardium‐targeting peptide CSTSMLKAC (IMTP). In vitro results showed that IMTP‐exosomes could be internalized by hypoxia‐injured H9C2 cells more efficiently than blank‐exosomes. Compared with blank‐exosomes, IMTP‐exosomes were observed to be increasingly accumulated in ischemic heart area (P<0.05). Meanwhile, attenuated inflammation and apoptosis, reduced fibrosis, enhanced vasculogenesis, and cardiac function were detected by mesenchymal stem cell–derived IMTP‐exosome treatment in ischemic heart area.ConclusionsOur research concludes that exosomes engineered by IMTP can specially target ischemic myocardium, and mesenchymal stem cell–derived IMTP‐exosomes exert enhanced therapeutic effects on acute myocardial infarction.

show abstract

Section: Introductionmentioning

confidence: 99%

Engineered Exosomes With Ischemic Myocardium‐Targeting Peptide for Targeted Therapy in Myocardial Infarction

Wang

Chen

Zhao

et al. 2018

JAHA

286

206

View full text Add to dashboard Cite

show abstract

“…However, the initial result is encouraging. Since exosomes have the ability to cross the BBB [43], it would be interesting to study the effect of WCFC on BDNF-mediated brain functionalities such as cognitive activity [47], appetite control [55], or modulation of neurodegenerative conditions [47,49,56,57]. …”

Section: Discussionmentioning

confidence: 99%

“…Likewise, exosomes released into blood may pass the BBB and deliver their exosomal content [48]. Such an arrangement would suggest that exosomes are of potential therapeutic value as a means to deliver substances to brain that do not normally cross the BBB [49].…”

Section: Introductionmentioning

confidence: 99%

Stimulatory Effect of Whole Coffee Fruit Concentrate Powder on Plasma Levels of Total and Exosomal Brain-Derived Neurotrophic Factor in Healthy Subjects: An Acute Within-Subject Clinical Study

Reyes-Izquierdo¹,

Argumedo²,

Shu³

et al. 2013

FNS

View full text Add to dashboard Cite

A pilot study by Reyes [1] previously showed that ingestion of single dose of whole coffee fruit concentrate (WCFC) powder increased blood levels of brain derived neurotrophic factor (BDNF) during the first 60 minutes after ingestion. In the present report, we performed a single dose, placebo-controlled, within-subject study to confirm and further investigate this effect. Twenty healthy subjects with ages ranging from 25 to 35 participated in this study. All fasted and resting subjects received placebo on Day 1, WCFC on Day 2, and a cup of freshly brewed coffee on Day 3. Treatment with WCFC resulted in a statistically significant increase in plasma BDNF compared to placebo (p = 0.0073) or coffee (p = 0.0219) during first 60 minutes. In addition, e isolated exosomes from serum and found that they contained BDNF. Furthermore, oral WCFC consumption acutely increased BDNF levels in serum exosomes. In summary, all presented results justify further clinical investigation of WCFC as a tool to manage BDNF-dependent health conditions.

show abstract

“…associated to beads, a polymer surface in a chip or a chromatography column) [77,78]. Of note, this method requires knowledge of specific EV markers, which despite many years of research [79] are still difficult to identify. To circumvent the lack of specific markers, a more general approach was recently presented in which antibodies are substituted by heparin as it appears to have a general affinity for EVs.…”

Section: Ev Purification Protocols and Stabilitymentioning

confidence: 99%

“…Additionally, the idea of creating EV mimicking vesicles, e.g. by means of sequential extrusion of cells through micro-and nanoporous filters [185,186] or by mixing synthetic components attempting to reproduce the most important EV characteristics [79,187], is gaining interest. However, the latter approach is difficult to implement as long as the knowledge on which components are essential for EV functionality is lacking or incomplete.…”

Section: (Nct02138331)mentioning

confidence: 99%

Therapeutic and diagnostic applications of extracellular vesicles

Stremersch

Smedt

Raemdonck

2016

Journal of Controlled Release

159

103

View full text Add to dashboard Cite

During the past two decades, extracellular vesicles (EVs) have been identified as important mediators of intercellular communication, enabling the functional transfer of bioactive molecules from one cell to another. Consequently, it is becoming increasingly clear that these vesicles are involved in many (patho)physiological processes, providing opportunities for therapeutic applications. Moreover, it is known that the molecular composition of EVs reflects the physiological status of the producing cell and tissue, rationalizing their exploitation as biomarkers in various diseases. In this review the composition, biogenesis and diversity of EVs is discussed in a therapeutic and diagnostic context. We describe emerging therapeutic applications, including the use of EVs as drug delivery vehicles and as cell-free vaccines, and reflect on future challenges for clinical translation. Finally, we discuss the use of EVs as a biomarker source and highlight recent studies and clinical successes.

show abstract

Microvesicles and exosomes: Opportunities for cell-derived membrane vesicles in drug delivery

Cited by 364 publications

References 128 publications

Engineered Exosomes With Ischemic Myocardium‐Targeting Peptide for Targeted Therapy in Myocardial Infarction

Engineered Exosomes With Ischemic Myocardium‐Targeting Peptide for Targeted Therapy in Myocardial Infarction

Stimulatory Effect of Whole Coffee Fruit Concentrate Powder on Plasma Levels of Total and Exosomal Brain-Derived Neurotrophic Factor in Healthy Subjects: An Acute Within-Subject Clinical Study

Therapeutic and diagnostic applications of extracellular vesicles

Contact Info

Product

Resources

About