Microvessel Density and Status of p53 Protein as Potential Prognostic Factors for Adjuvant Anthracycline Chemotherapy in Retrospective Analysis of Early Breast Cancer Patients Group

Biesaga, Beata; Niemiec, Joanna; Ziobro, Marek

doi:10.1007/s12253-012-9525-9

Cited by 20 publications

(29 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mean value of MVD found by us was within the range of values reported by other authors (discussed in [23,24]). We confirmed no correlation between LVD (or DPV) and MVD [25].…”

Section: Discussionsupporting

confidence: 92%

Lymphangiogenesis assessed using three methods is related to tumour grade, breast cancer subtype and expression of basal marker

Niemiec¹,

Adamczyk²,

Ambicka³

et al. 2012

pjp

Self Cite

View full text Add to dashboard Cite

Lymphangiogenesis is a potential indicator of cancer patients' survival. However, there is no standardisation of methodologies applied to the assessment of lymphatic vessel density. In 156 invasive ductal breast cancers (T ≥ 1/N+/M0), lymphatic and blood vessels were visualised using podoplanin and CD34, respectively. Based on these markers expression, four parameters were assessed: (i) distribution of podoplanin-stained vessels (DPV) -the percentage of fields with at least one lymphatic vessel (a simple method proposed by us), (ii) lymphatic vessel density (LVD), (iii) LVD to microvessel density ratio (LVD/MVD) and (iv) the expression of podoplanin in cancer-associated fibroblasts. Next, we estimated relations between the above-mentioned parameters and: (i) breast cancer subtype, (ii) tumour grade, and (iii) basal markers expression. We found that intensive lymphangiogenesis, assessed using all studied methods, is positively related to high tumour grade, triple negative or HER2 subtype and expression of basal markers. Whereas, the absence of podoplanin expression in fibroblasts of cancer stroma is related to luminal A subtype, low tumour grade or lack of basal markers expression. Distribution of podoplanin-stained vessels, assessed by a simple method proposed by us (indicating the percentage of fields with at least one lymphatic vessel), might be used instead of the "hot-spot" method.

show abstract

“…The mean value of MVD found by us was within the range of values reported by other authors (discussed in [23,24]). We confirmed no correlation between LVD (or DPV) and MVD [25].…”

Section: Discussionsupporting

confidence: 92%

Lymphangiogenesis assessed using three methods is related to tumour grade, breast cancer subtype and expression of basal marker

Niemiec¹,

Adamczyk²,

Ambicka³

et al. 2012

pjp

Self Cite

View full text Add to dashboard Cite

show abstract

“…We have found a statistically significant relationship between increasing tumor size and mVD (p=0.001). There are consistent studies (19-21) with our results in the literature as well as some studies (16,22) found no significant relationship between mVD and tumor size and some studies found an inverse correlation between them (23).…”

Section: Discussionsupporting

confidence: 91%

“…In the literature, some studies have reported that there is no relationship between mVD and prognostic parameters such as patient's age (25,27), histological grade (28), lymphovascular invasion (9), estrogen and progesterone receptor status (27), HEr2/neu overexpression (27). On the other hand, there are also some other studies that found a significant relationship between high mVD and patient's age (29), high histologic grade (16,25,27), presence of lymphovascular invasion (30), estrogen (16,22) and progesterone receptor negativity (22) and HEr2/neu overexpression (31).…”

Section: Discussionmentioning

confidence: 96%

Comparison of microvessel density with prognostic factors in invasive ductal carcinomas of the breast

Şener¹,

Şipal²,

Gündoğdu³

2016

TJPATH

View full text Add to dashboard Cite

Objective: Angiogenesis plays a key role in tumor growth and metastasis. Determination of microvessel density is the most common technique used to evaluate the amount of the intratumoral angiogenesis in breast cancer. We have aimed to investigate the relationship with tumor angiogenesis and prognostic parameters in breast invasive ductal carcinomas. Material and Method:In this study, a total of 100 invasive ductal carcinoma patients, who were diagnosed at the Department of Pathology, Ataturk university Faculty of medicine between the years 2003-2008, were re-evaluated. Patient characteristics and clinicopathological findings were obtained from archival records. In the present study, microvessel density was determined by immunohistochemical staining by using anti-CD34 monoclonal antibody in the paraffin blocks. First, the most vascular area was selected in the tumor under a low magnification (40x) by a light microscope and then microvessels were counted under a higher magnification (200x). Patients were classified as low and high microvessel density depending on their microvessel counts. Chi-square test and multivariate linear regression analysis were used for statistical analysis (p≤0.05). Results:We have determined that microvessel density increases as tumor size increases (p=0.001). microvessel density was higher in patients with at least 10 lymph node metastases compared to those with no metastasis (p=0.05). However, there was no statistically significant difference between microvessel density and other prognostic factors such as histological grade, nuclear grade, patient age, vascular invasion, estrogen, progesterone receptor status, HEr2/neu expression. Conclusion:In our study, we have found that microvessel density is associated with tumor size and lymph node metastasis in patients with invasive ductal carcinoma.

show abstract

“…The prognostic value of MVD has remained controversial since its introduction twenty years ago: some initial studies have suggested that the MVD is a major independent prognostic factor for overall survival in invasive breast cancer (de Jong et al, 2000), but other studies have concluded that the MVD does not predict poor outcomes of invasive breast cancer (Kanjanapanjapol et al, 2007). Some recent research results have suggested that higher MVD is a favorable prognostic factor for early advanced breast cancer patients after adjuvant anthracycline-based chemotherapy (Biesaga et al, 2012). The authors of that study have speculated that differential distribution of MVD in tumors might be one of the reasons for different outcomes.…”

Section: Discussionmentioning

confidence: 99%

Effects of Differential Distribution of Microvessel Density, Possibly Regulated by miR-374a, on Breast Cancer Prognosis

Zhang²,

Zhang³

et al. 2013

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: The discovery that microRNAs (miRNAs) regulate proliferation, invasion and metastasis provides a principal molecular basis of tumor heterogeneity. Microvessel distribution is an important characteristic of solid tumors, with significant hypoxia occurring in the center of tumors with low blood flow. The distribution of miR-374a in breast tumors was examined as a factor likely to be important in breast cancer progression. Methods: Breast tissue samples from 40 patients with breast cancer were classified into two groups: a highly invasive and metastatic group (HIMG) and a low-invasive and metastatic Group (LIMG). Samples were collected from the center and edge of each tumor. In each group, six specimens were examined by microRNA array, and the remaining 14 specimens were used for real-time RT-qPCR, Western blot and immunohistochemical analyses. Correlation analysis was performed for the miRNAs and target proteins. Follow-up was carried out during 28 months to 68 months after surgery, and survival data were analyzed. Results: In the LIMG, the relative content of miR-374a was lower in the center of the tumor than at its edge; in the HIMG, it was lower at the edge of the tumor, and miR-374a levels were lower in breast cancer tissues than in normal tissues. There was no difference between VEGF-A and VCAM-1 mRNA levels at the edge and center of the tumor; however, we observed a significant difference between VEGF-A and VCAM-1 protein expression levels in these two regions. There was a negative correlation between miR-374a and target protein levels. The microvessel density (MVD) was lower in the center of the tumor than at its edge in HIMG, but the LIMG vessels were uniformly distributed. There was a significant positive correlation between MVD and the number of lymph node metastases (Pearson correlation, r=0.912, P<0.01). The median follow-up time was 48.5 months. LIMG had higher rate of disease-free survival (100%, P=0.013) and longer median survival time (66 months) than HIMG, which had a lower rate of 75% and shorter median survival time (54 months). Conclusions: Our data demonstrated miR-374a to be differentially distributed in breast cancer; VEGF-A and VCAM-1 mRNA had coincident distribution, and the distribution of teh respective proteins was uneven and opposite to that for the miR-374a. These data might explain the differences in the distribution of MVD in breast cancer and variation in breast cancer prognosis.

show abstract

Microvessel Density and Status of p53 Protein as Potential Prognostic Factors for Adjuvant Anthracycline Chemotherapy in Retrospective Analysis of Early Breast Cancer Patients Group

Cited by 20 publications

References 34 publications

Lymphangiogenesis assessed using three methods is related to tumour grade, breast cancer subtype and expression of basal marker

Lymphangiogenesis assessed using three methods is related to tumour grade, breast cancer subtype and expression of basal marker

Comparison of microvessel density with prognostic factors in invasive ductal carcinomas of the breast

Effects of Differential Distribution of Microvessel Density, Possibly Regulated by miR-374a, on Breast Cancer Prognosis

Contact Info

Product

Resources

About