Microvessel density for melanoma prognosis

Depasquale, Ivan; Thompson, W. D.

doi:10.1111/j.1365-2559.2005.02193.x

Cited by 38 publications

(36 citation statements)

References 30 publications

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“…The Chalkley vessel counting technique is an objective, easy, and reproducible method and provides both a quantitative and qualitative assessment. 19 The study by Hansen et al 22 demonstrated prognostic significance of tumor vascularity in breast carcinomas, when assessed by the Chalkley counting technique in contrast to assessing microvessel density as originally presented by Weidner et al 12 The Chalkley method therefore was adopted in this study.…”

Section: Discussionmentioning

confidence: 97%

Vascular Density Has Prognostic Value in Merkel Cell Carcinoma

Ng¹,

Beer²,

Murray³

2008

The American Journal of Dermatopathology

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Merkel cell carcinomas are aggressive tumours for which histological prognostic factors need to be established. This study examines the prognostic role of vascular density, based on CD34 immunohistochemical staining in Merkel cell carcinoma. Thirty-six cases of Merkel cell carcinoma were immunohistochemically stained for the endothelial marker CD34. Vascular density was assessed in the tumor and stroma with a Chalkley eyepiece graticule. The scores of vascular density were correlated with other clinical and histological parameters to determine the prognostic significance of tumor vascularity. Increased vascular density was shown to be significantly associated with a worse prognosis (P = 0.005). A 1-unit increase in total vessel score was associated with a 3.9 times increase in the risk of death (95% hazard ratio confidence limits 1.50-10.32). Other factors associated with a worse outcome included tumor size (P = 0.05), the presence of lymphovascular invasion (P = 0.03), and tumor mast cell count (P < 0.002). Increased vascular density is associated with a worse prognosis in Merkel cell carcinomas. Assessment of vascular density may assist in predicting clinical behavior in these tumors and in evaluating the effects of adjuvant therapy.

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Section: Discussionmentioning

confidence: 97%

Vascular Density Has Prognostic Value in Merkel Cell Carcinoma

Ng¹,

Beer²,

Murray³

2008

The American Journal of Dermatopathology

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“…As in large-scale studies of breast carcinoma, exhibiting a significant and independent prognostic value for survival, Depasquale and Thompson [38] concluded that tumor microvessel counts, by the Chalkley technique, were related to a risk of recurrence in melanomas thicker than 2.0 mm.…”

Section: Discussionmentioning

confidence: 99%

“…Applying this counting technique to microvessels makes it possible to achieve greater correspondence between the points on the graticule and the vascular lumens, thus reflecting not just the total number of microvessels, but the relative area they occupy [38]. This method is considered more objective and has been indicated as the most powerful predictive factor of the prognosis of many neoplasms [39], as in studies of breast carcinomas showing a significant and independent prognosis for survival.…”

Section: Discussionmentioning

confidence: 99%

Immunoexpression of endoglin in brain metastasis secondary to malignant melanoma: evaluation of angiogenesis and comparison with brain metastasis secondary to breast and lung carcinomas

Salgado

Toscani

Silva

et al. 2007

Clin Exp Metastasis

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Brain metastases are linked to poor prognosis. After carcinomas of the lung and breast, malignant melanomas (MM) are the next type of neoplasm with the higher metastatic dissemination involving the central nervous system and that has the worst prognosis after metastasis has been diagnosed. Angiogenesis has been linked to tumor growth and metastasis. Among the immunomarkers of angiogenesis, endoglin (CD105) is the most specific antibody, since it is a marker for tumor endothelium, and expression of CD105 has been observed to be associated with prognosis in several types of tumor, which is not always observed in melanomas. This study investigated angiogenesis in brain metastasis secondary to malignant melanomas and compared these with brain metastasis secondary to carcinomas of the lung and breast, through expression of CD105 (endoglin). The study investigated 93 cases of brain metastasis secondary to MM (33) and carcinomas of the lung (31) and breast (29), assessing endoglin immunoexpression, number of microvessels and diameter of tumor vessels. Tumor microvessels were counted using a modified version of the Chalkley technique. The observed difference between MM and breast carcinoma was statistically significant (P = 0.026). The difference between MM and lung carcinoma was not significant (P = 0.218). Vascular diameter observation revealed no statistical difference between the vascular size of neoplastic vessels in MM and in breast and lung carcinomas. Of the tumors investigated here, malignant melanomas were shown to have the lowest number of microvessels and had intermediate tumor vessel diameter as compared to carcinomas of the lungs and breast. Such results were not expected to be found in neoplasms such as melanomas that, besides presenting high dissemination capacity, have a high index of hemorrhage secondary to brain metastasis.

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“…More specifically, to become clinically relevant, a tumor requires persistent neovascularization for its growth and survival (30). Neoplastic vessel density is correlated with an adverse prognosis in many types of adult and pediatric solid tumors (31,32).…”

Section: Discussionmentioning

confidence: 99%

Enhanced Antitumor Efficacy of Clinical-Grade Vasculature-Targeted Liposomal Doxorubicin

Pastorino

Paolo

Piccardi³

et al. 2008

Clinical Cancer Research

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Purpose: In vivo evaluation of good manufacturing practice-grade targeted liposomal doxorubicin (TVT-DOX), bound to a CD13 isoform expressed on the vasculature of solid tumors, in human tumor xenografts of neuroblastoma, ovarian cancer, and lung cancer. Experimental Design: Mice were implanted with lung, ovarian, or neuroblastoma tumor cells via the pulmonary, peritoneal, or orthotopic (adrenal gland) routes, respectively, and treated, at different days post inoculation, with multiple doses of doxorubicin, administered either free or encapsulated in untargeted liposomes (Caelyx) or inTVT-DOX. The effect of TVT-DOX treatment on tumor cell proliferation, viability, apoptosis, and angiogenesis was studied by immunohistochemical analyses of neoplastic tissues and using the chick embryo chorioallantoic membrane assay. Results: Compared with the three control groups (no doxorubicin, free doxorubicin, or Caelyx), statistically significant improvements in survival was seen in all three animal models following treatment with 5 mg/kg (maximum tolerated dose) of TVT-DOX, with long-term survivors occurring in the neuroblastoma group; increased survival was also seen at a dose of 1.7 mg/kg in mice bearing neuroblastoma or ovarian cancer. Minimal residual disease after surgical removal of neuroblastoma primary mass, and the enhanced response toTVT-DOX, was visualized and quantified by bioluminescence imaging and with magnetic resonance imaging.When treated withTVT-DOX, compared with Caelyx, all three tumor models, as assayed by immunohistochemistry and chorioallantoic membrane, showed statistically significant reductions in cell proliferation, blood vessel density, and microvessel area, showing increased cell apoptosis. Conclusion: TVT-DOX should be evaluated as a novel angiostatic strategy for adjuvant therapy of solid tumors.

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Microvessel density for melanoma prognosis

Cited by 38 publications

References 30 publications

Vascular Density Has Prognostic Value in Merkel Cell Carcinoma

Vascular Density Has Prognostic Value in Merkel Cell Carcinoma

Immunoexpression of endoglin in brain metastasis secondary to malignant melanoma: evaluation of angiogenesis and comparison with brain metastasis secondary to breast and lung carcinomas

Enhanced Antitumor Efficacy of Clinical-Grade Vasculature-Targeted Liposomal Doxorubicin

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