Microviscosity of human erythrocytes studied using hypophosphite two-spin order relaxation

Price, William S.; Perng, Baw Ching; Tsai, C. L.; Hwang, Lian-Pin

doi:10.1016/s0006-3495(92)81867-2

Cited by 12 publications

(1 citation statement)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In many cases, such differences do not exist or they are very small (e.g., water). However, there are generally differences in the diffusion coefficient and in the available diffusion space for the probe molecule between the intra-and extracellular compartments (e.g., Price et al, 1989a,b;Price and Kuchel, 1990b;Price et al, 1992). Such differences may be exploited with pulsed field gradient nuclear magnetic resonance (PFG NMR, also referred to as pulsed gradient spin-echo or PGSE NMR; Kärger et al, 1988;Stilbs, 1987;Callaghan, 1991;Price, 1996Price, , 1997 to separate the domains (Andrasko, 1976;Price and Kuchel, 1990b;Van Zijl et al, 1991;Potter et al, 1996), even in the absence of chemical shift and/or relaxation rate differences.…”

Section: Introductionmentioning

confidence: 99%

A Model for Diffusive Transport through a Spherical Interface Probed by Pulsed-Field Gradient NMR

Price¹,

Barzykin

Hayamizu

et al. 1998

Biophysical Journal

105

104

View full text Add to dashboard Cite

In biological systems, because of higher intracellular viscosity and/or the restriction of the diffusion space inside cells, the (apparent) diffusion coefficient of an intracellular species (e.g., water) is generally smaller than when it is in the extracellular medium. This difference affects the spin-echo signal attenuation in the pulsed field gradient NMR experiment and thus affords a means of separating the intracellular from the extracellular species, thereby providing a basis for studying transmembrane transport. Such experiments have commonly been analyzed using the macroscopic model of Kä rger (see Adv. Magn. Reson. 21:1-89 (1988)). In our previous study, we considered a microscopic model of diffusive transport through a spherical interface using the short gradient pulse approximation (J. Magn. Reson. A114:39-46 (1995)). The spins in the external medium were modeled with the "partially absorbing wall" condition or as having a small but finite lifetime. In the present paper, we extend our treatment to the case in which there is no limitation upon the lifetime in either medium. We also consider a simple modification of Kä rger's model that more properly accounts for the restricted intracellular diffusion. Importantly, it was found that the exact solution within the short gradient pulse approximation developed here and the modified Kä rger model are in close agreement in the (experimentally relevant) long-time limit. The results of this study show that when there is no limitation upon the lifetime of the transported species in either phase, the spin-echo attenuation curve is very sensitive to transport.

show abstract

Section: Introductionmentioning

confidence: 99%

A Model for Diffusive Transport through a Spherical Interface Probed by Pulsed-Field Gradient NMR

Price¹,

Barzykin

Hayamizu

et al. 1998

Biophysical Journal

105

104

View full text Add to dashboard Cite

show abstract

Pulsed‐field gradient nuclear magnetic resonance as a tool for studying translational diffusion: Part 1. Basic theory

Price¹

1997

Concepts Magn. Reson.

336

470

View full text Add to dashboard Cite

Some Recent Developments in NMR Approaches for Studying Liquid Molecular Dynamics and Their Biological Applications

Price

Hwang

1992

J Chinese Chemical Soc

View full text Add to dashboard Cite

Two aspects of NMR relaxation are reviewed and their use in studying molecular dynamics in the liquid state and their application to biological systems are discussed. The first aspect concerns the use of ‘null‐point spectra’ for observing the effects of higher rank multipoles (tensors) in the relaxation of quadrupolar nuclei. The second aspect concerns the effects of cross‐correlation in homo‐ and heteronuclear spin systems. The application of null point spectral analysis to study cross‐correlation in homonuclear spin systems is also discussed. Analysis of the effects of higher rank multipoles and of cross‐correlation effects are shown to provide substantially more structural and motional information about spin systems than that available from traditional relaxation measurements. While similar in information content to multiple quantum techniques, the null point technique is experimentally less demanding. Over the past several years, work in this laboratory has been heavily involved in the theoretical formulation and the application of these techniques to biological systems. Illustrations of the use of these techniques to biological systems are drawn from our recent work.

show abstract

Microviscosity of human erythrocytes studied using hypophosphite two-spin order relaxation

Cited by 12 publications

References 47 publications

A Model for Diffusive Transport through a Spherical Interface Probed by Pulsed-Field Gradient NMR

A Model for Diffusive Transport through a Spherical Interface Probed by Pulsed-Field Gradient NMR

Pulsed‐field gradient nuclear magnetic resonance as a tool for studying translational diffusion: Part 1. Basic theory

Some Recent Developments in NMR Approaches for Studying Liquid Molecular Dynamics and Their Biological Applications

Contact Info

Product

Resources

About