Microwave assisted extraction of polysaccharides from Yupingfeng powder and their antioxidant activity

Abstract

Background:Microwave-assisted reflux extraction of polysaccharides YPF-P from the famous Chinese traditional drug, Yupingfeng powder, optimization of extracting conditions and evaluation of their antioxidant activity were conducted in this study.Results:Single factor effect trends were achieved through yields and contends of YPF-P obtained from different extracting conditions. Then through a three-level, four-variable Box-Behnken design of response surface methodology adopting yield as response, the optimal conditions were determined as follows: Material/solvent ratio 1:23.37, microwave power 560 W, Extraction temperature 64°C, and extraction time 9.62 min. Under the optimal conditions, the YPF-P extraction yield was 3.23%, and its content was detected as 38.52%. In antioxidant assays, the YPF-P was tested to possess 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities with an IC50 value of 0.262 mg/ml. In addition, YPF-P was also proved to have relatively low ferric reducing antioxidant power (FRAP), compared to Vc, through FRAP assay.Conclusion:In the microwave assisted reflux extraction research, good YPF-P yield was achieved from materials with relatively low YPF-P content. And for the first time, both DPPH and FRAP assays were conducted on YPF-P, which proved that the antioxidant activity of YPF-P contributed to the functions of this medicine.

Date pomace polysaccharide: ultrasonic-assisted deep eutectic solvent extraction, physicochemical properties, biological activities, gut microbiota modulation, and rheological properties

“…SD and SAS activities are comparable to previous study [16]. The presence of functional groups in polysaccharides makes them effective scavengers of hydroxyl radicals [8]. UPS exhibits higher HP and HRS activities at 1 mg/mL than the 25.5% and 26.6% inhibition at 5 mg/mL [36].…”

“…Polysaccharide chelation of metallic ions has garnered attention, influenced by hydroxyl group accessibility and cross-bridges with divalent ions [8]. The metal chelation (MC) assay shows UPS's effective binding capacity with ferrozine, surpassing previous reports [48].…”

“…Despite their importance, date pomace (DP) polysaccharides remain an underutilized resource within the food industry. Conventional extraction methods for polysaccharides, which typically rely on solvent use and are energy-intensive, are fraught with inefficiencies, thus bolstering the transition towards more eco-friendly extraction techniques [8].…”

Clinical Features and Rules of Chinese Herbal Medicine in Diabetic Peripheral Neuropathy Patients

“…Astragalus membranaceus has the effect of two-way regulation of excessive sweat and reduced sweat. These two herbals may also have good clinical effect on antioxidation through polysaccharides in Yupingfeng power [ 36 ]. This may regulate abnormal skin sweating in DPN.…”

Yupingfeng polysaccharide promote the growth of chickens via regulating gut microbiota

“…It has the functions of immune regulation (18), anti-virus (19), anti-tumor (20), and anti-aging (21). Yupingfeng polysaccharide is the main substance in the alcohol-precipitation part of Yupingfeng powder, which has many biological functions such as enhancing the body's immunity (22), repairing the intestinal barrier (23), and enhancing the body's antioxidant capacity (24). For example, YPF-P significantly increased the thymus index and promoted the secretion of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), immunoglobulin A (IgA), immunoglobulin A (IgG) and immunoglobulin M (IgM) and other immune factors and antioxidant factors in the blood (25).…”