Microwave digestion method for phosphorus determination of fish tissue

Abstract

Abstract-A simple time-saving method is described for the digestion of fish tissue for spectrophotometric determination of phosphorus. Microwave digestion of fish tissue was compared to the commonly used method of ashing tissue in a muffle furnace. Microwave digestion produced significantly higher phosphorus recoveries and took only half the time to run the same number of samples compared to ashing.

Using high-pressure teflon bomb digestion in phosphorus determination of aquatic animals

“…Mean recovery rate for standard material is similar to that of obtained following microwave oven digestion (90-95%) and is by 14% higher than the recovery rate (80%) found using ashing method combined with nitric acid hydrolysis (Tanner et al, 1999). Mean recovery from standard material was close to the certified value following correction with the blank value, which supports the theory of P-independent MoO 4 2x ion reduction (Going and Eisenreich, 1974;Pai et al, 1990).…”

“…The variety in different P digestion methods clearly reveals that researchers are not consistent in this issue (Tanner et al, 1999;Sterner and George, 2000;Sereda et al, 2008), thus we support international standardization to achieve results comparable among laboratories. We have broadened the application of high-pressure bomb digestion to give an alternative tool and reference for limnologists doing measurements on P stoichiometry of any kind of aquatic animals.…”

“…Frost et al (2003) digested aquatic invertebrate samples by autoclaving with a potassium-persulfate digestion reagent. Tanner et al (1999Tanner et al ( , 2000 and Reis et al (2008) used concentrated acids and microwave oven treatment to digest dried biological samples. These procedures are based on the joint capacity of heat, pressure and oxidizing media to disassemble sample materials.…”

“…Major nutrients, such as carbon and nitrogen can be measured in dried-homogenized samples with good accuracy and precision in a relatively short time using carbon-hydrogennitrogen analyzers (Tanner et al, 1999;Sterner and George, 2000;Pilati and Vanni, 2007;Le Loc'h et al, 2008). In contrast, phosphorus (P) analysis is more timeconsuming and requires that animal tissues are digested before colorimetric measurement.…”

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“…Lipid was determined with ether extraction using an automatic extraction system (ANKOMXT15 Extractor, ANKOM Technology, Macedon, USA). Phosphorus analysis of the experimental diets was conducted according to Tanner et al (1999). Amino acid compositions of ingredients, experimental diets were determined using an automatic amino acid analyzer (JLC-500/v; Jeol, Tokyo, Japan) following digestion of samples with 4N methanesulfonic acid for 24 hours at 110 ᴼC.…”