Microwave Digestion of Environmental and Natural Waters for Selenium Speciation

Wang, Zijian; Gao, Yang; Belzile, Nelson

doi:10.1021/ac010330h

Cited by 16 publications

(10 citation statements)

References 14 publications

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“…The actual chemical environment in the digestion vial is greatly dependent on the ratio of reagents, their concentration and quantity, the digestion temperature, the nature and the amount of sample, the by-products generated during the sample decomposition, the configuration of MW apparatus and energy output, among others. For example, our observation was different from that of Wang et al [22], who have reported that 100% of Se was Se (IV) in a digestion system of H 2 O 2 :H 2 SO 4 (v:v = 5:1). This discrepancy might be due to the differences in the sample nature, the configuration of digestion apparatus, quantity of digestion reagents and digestion program employed.…”

Section: Selection Of Chemical Digestion Systemscontrasting

confidence: 99%

“…Furthermore, according to our knowledge on conventional MW digestion, a 10.0 mL of 15.4 M HNO 3 :30% (w/v) H 2 O 2 (4:1) digestion also results with Se (IV) as a final product of biological sample digestion even without the addition of NaCl. In another study using a conventional MW digestion system with 100 mL vial and different digestion reagents, the final decomposition product of Se in the tested Se containing substances (seleno-methionine, rice flour) was found to be Se (IV) when 0.5 mL of mixed H 2 O 2 :H 2 SO 4 (v:v = 5:1) was used [22]. Yet in another study, an on-line prereduction of Se (VI) to Se (IV) by thiourea was proposed.…”

Section: Introductionmentioning

confidence: 99%

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Low volume microwave digestion and direct determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry

Zhao

Chen

Belzile

et al. 2010

Analytica Chimica Acta

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Section: Selection Of Chemical Digestion Systemscontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low volume microwave digestion and direct determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry

Zhao

Chen

Belzile

et al. 2010

Analytica Chimica Acta

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“…During the heating period the digestion tubes were gently shaken every after one hour to make the contents homogeneous and to enhance fume release. The procedure followed in the digestion with slight modification of the procedure described by Wang et al (2001). After completion of the digestion process the samples were then ready for dilution.…”

Section: Digestion Of Samplesmentioning

confidence: 99%

Protective Role of Spirulina and Vitamin E against Arsenic Toxicity in Rats

Gofur¹,

Sayed²,

Khair³

et al. 2015

Asian J. of Animal Sciences

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Arsenic is one of the most sensitive environmental issues in Bangladesh; even it is a major health concern in Asia. Spirulina and vitamin E have been considered as a potential therapeutic supplement due to its ability to minimize several element induced toxicities in various species including man. The study was performed to evaluate the role of spirulina (Spirulina platensis) and vitamin E in prevention of arsenic toxicity in different groups (T 0-4 , n = 60) of Long-Evans rats. T 0 was control group, T 1 was treated with sodium arsenite, T 2 was treated with sodium arsenite plus spirulina, T 3 was treated with sodium arsenite plus vitamin E and T 4 was treated with sodium arsenite plus vitamin E plus spirulina daily for 63 days. Sodium arsenite was at 4 mg kgG 1 b.wt., spirulina was at 1 g kgG 1 feed and vitamin E was at 200 mg kgG 1 feed. Samples were collected on day 21, 42 and 63. Arsenic was detected from tissue samples by Hydride Generation Atomic Absorption Spectrophotometer (HGAAS). Sodium arsenite feeding in rats caused chronic arsenic toxicity and the arsenic content in tissues (blood, lung, liver and kidney) of the exposed rats were significantly higher than control rats. Spirulina and vitamin E treatments significantly lowered the arsenic content in tissues. Arsenic caused hepatic and renal dysfunction but spirulina and vitamin E improved the hepatic and renal functions. Spirulina feeding was more effective than vitamin E and their combined treatment was more effective compare to their single treatment. The study demonstrates the role of spirulina and vitamin E in the reduction of toxicity of arsenic.

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“…Recently, a microwave preparation procedure was developed for selenium speciation in natural and drinking waters. The procedure used HNO 3 as the digesting reagent and provided a detection limit of 0.0066 ng (17). It could provide a much more precise tool for the estimation of a selenium-containing protein after SDS-PAGE separation.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Selenium-Containing Proteins in Human Serum

Gao

Liu

Deng

et al. 2004

BTER

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Selenium-containing proteins in human serum of four volunteers in Beijing were separated and purified by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Selenium contents in the proteins were quantified by high-performance liquid chromatography (HPLC) coupled with a fluorescence detector (FLD) after pretreatment with a microwave digestion system and derivatization with 2,3-diaminonaphthalene (DAN). Five selenium-containing proteins with apparent molecular weights (MWs) of 68+/-3, 57.5+/-2.5, 47+/-2, 41+/-1, and 21+/-1 kDa, respectively, were detected. By comparison with known data on serum selenium-containing proteins, the 68+/-3 kDa protein should belong to albumin, which took 6.3-9.8% of the total serum Se. The 57.5+/-2.5 kDa protein should be selenoprotein P and the 47+/-2 kDa protein was believed to be an isoform of selenoprotein P. The sum of Se in selenoprotein P and its isomer took about 41.1-69.3% and was the major form of human serum selenium. The 21+/-1 kDa protein should be plasma glutathione peroxidase (p-GPx) and its Se content was about 21.1-24.3%. Also, protein of 41+/-1 kDa should be a selenium-containing protein that, to our best knowledge, was reported for the first time. The Se percentage in this protein corresponded to 12.6-20.4% of total human serum Se.

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Microwave Digestion of Environmental and Natural Waters for Selenium Speciation

Cited by 16 publications

References 14 publications

Low volume microwave digestion and direct determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry

Low volume microwave digestion and direct determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry

Protective Role of Spirulina and Vitamin E against Arsenic Toxicity in Rats

Distribution of Selenium-Containing Proteins in Human Serum

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