Microwave-Facilitated SPOT-Synthesis of Antibacterial Dipeptoids

Schneider, Anne C.; Fritz, Daniel; Vasquez, Joseph K.; Vollrath, Sidonie B. L.; Blackwell, Helen E.; Bräse, Stefan

doi:10.1021/acscombsci.7b00096

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…Although some publications describe the application of elevated temperature for the preparation of the membrane and for amino acid couplings by heating using an infrared light or a microwave [31,32], we will describe here only the synthesis at room temperature (at approx. 22-24 C).…”

Section: Methodsmentioning

confidence: 99%

SPOT Synthesis: The Solid-Phase Peptide Synthesis on Planar Surfaces

Winkler

2019

Methods in Molecular Biology

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Peptide libraries are a highly useful tool for drug development. While most preparations of peptide libraries are laborious during either the synthesis or its screening, the SPOT synthesis offers the possibility of directly synthesizing large numbers of peptides on a planar surface. As a positionally addressable, multiple solidphase synthesis technique, the synthesis allows a very convenient handling during the screening of that peptide library in a form of an array. This publication will provide protocols for the basic procedures of the SPOT synthesis and references to some important literature regarding that technique and its application.

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Section: Methodsmentioning

confidence: 99%

SPOT Synthesis: The Solid-Phase Peptide Synthesis on Planar Surfaces

Winkler

2019

Methods in Molecular Biology

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“…Schneider et al . presented MW‐assisted SPOT‐synthesis of dipeptoids on the functionalized cellulose supports [17c] . SPOT‐synthesis allows for rapid synthesis and screening of large libraries [19] .…”

Section: Peptide Backbone Modifications and Solid‐phase Synthesis Techniquesmentioning

confidence: 99%

Solid‐Phase Synthesis of Peptidomimetics with Peptide Backbone Modifications

2021

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Peptidomimetics are a class of compounds with promising pharmacological properties. Peptidomimetics reduce limitations of peptides including low bioavailability, poor stability, and poor cell‐permeability. Peptide backbone modifications are a frequently used manipulation to reach desirable properties of the peptide molecules. The development of accessible synthetic methodologies plays an important role in peptidomimetic progress. Synthesis of peptidomimetics proceeds via solution and solid‐phase synthesis strategies. Solid‐phase organic synthesis serves as a powerful tool for the preparation of peptidomimetic molecules, thus, numerous strategies have been developed over the years. In this review, we discuss solid‐phase synthetic approaches for peptide backbone modifications that were presented in the last two decades.

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“…85 These hydrophilic membranes allowed for synthesizing peptoid libraries by pipetting single droplets of non-volatile reagent solutions to defined spots on the solid support. 46,85 Initially, this so-called SPOT-synthesis was discovered by Ronald Frank. 86 .…”

Section: Spot Arraysmentioning

confidence: 99%

“…Microwave-assisted techniques can even accelerate the entire process. [45][46][47][48][49] Moreover, the submonomer method is suitable for automatization. Zuckermann developed the machine synthesis of peptoid oligomers on a common peptide synthesizer.…”

Section: Introductionmentioning

confidence: 99%