In this research, the effect of microwave radiation simultaneous with acidizing on the carbonate rock surface has been investigated. Saturated rock sections were first immersed in the acid solution, and then, exposed to the microwave at 1300 W and 780 W for 1, 2, and 3 min. The surface wettability and hydrocarbon desorption from the rock surface were respectively investigated by the contact angle measurement and Attenuated Total Reflectance-Fourier Transform Infra-Red (ATR-FTIR) spectroscopy. It was observed that the angle under the microwave in the vicinity of the acidic solution improves toward waterwetness and becomes oil-wet. The reduction of the contact angle in the combined mode is tenser due to the simultaneous effect of acid and microwave radiation. According to the ATR-FTIR spectrums, the microwave radiation of the sections during the acid treatment led to desorption of aromatic compounds, long alkanes, polar bonds (O-H and N-H), and the compounds with C=O functional groups. The electrically polar hydrocarbons have more