Microwave irradiation and in vitro release of enzymes from hepatic lysosomes

Self Cite

Liver mitochondria were exposed in vitro at 30 degrees C to microwave radiation (2.45 GHz) during the following states of respiration: resting, state 1; substrate dependent, state 2; ADP stimulated, state 3; and ADP depleted, state 4. At 10 or 100 mW/g, with succinate as substrate, no effect of exposure was observed on states 1-4 or the respiratory control index (state 3/state 4) of either tightly or loosely coupled mitochondria. When glutamate was used as substrate, no effects were observed at 10 mW/g. However, in the loosely coupled mitochondria the 100 mW/g exposure produced an increase in states 2 and 4 and a decrease in the respiratory control index. The results suggest that the function of loosely coupled mitochondria can be affected at high power levels of microwave radiation.

“…1) described in detail elsewhere [Galvin et al, 1980;Galvin et al, 1981a,b]. Throughout exposure, >95% of the the sample was in the microwave field.…”

Section: Microwave Exposurementioning

confidence: 99%

“…The mitochondrial suspension was stirred continuously and irradiated in glass sample tubes inserted through a waveguide chamber ( Fig. 1) described in detail elsewhere [Galvin et al, 1980;Galvin et al, 1981a,b]. Throughout exposure, >95% of the the sample was in the microwave field.…”

Section: Microwave Exposurementioning

confidence: 99%

In vitro effects of microwave radiation on rat liver mitochondria

Dutton

Galvin

McRee

1984

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Stress proteins are not induced in mammalian cells exposed to radiofrequency or microwave radiation

Cleary

Cao

Liu

et al. 1997

The induction of stress proteins in HeLa and CHO cells was investigated following a 2 h exposure to radiofrequency (RF) or microwave radiation. Cells were exposed or sham exposed in vitro under isothermal (37 ± 0.2 °C) conditions. HeLa cells were exposed to 27‐ or 2450 MHz continuous wave (CW) radiation at a specific absorption rate (SAR) of 25 W/kg. CHO cells were exposed to CW 27 MHz radiation at a SAR of 100 W/kg. Parallel positive control studies included 2 h exposure of HeLa or CHO cells to 40 °C or to 45 μM cadmium sulfate. Stress protein induction was assayed 24 h after treatment by electrophoresis of whole‐cell extracted protein labeled with [35S]‐methionine. Both cell types exhibited well‐characterized responses to the positive control stresses. Under these exposure conditions, neither microwave nor RF radiation had a detectable effect on stress protein induction as determined by either comparison of RF‐exposed cells with sham‐exposed cells or comparison with heat‐stressed or Cd++ positive control cells. Bioelectromagnetics 18:499–505, 1997. © 1997 Wiley‐Liss, Inc.

Influence of 2.45‐GHz CW microwave radiation on spontaneously beating rat atria

Galvin

Dutton

McRee

1982

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The chronotropic and inotropic effects of 2.45-GHz continuous wave (CW) microwave radiation were investigated in the isolated spontaneously beating rat atria. Isolated atria were placed in specially designed tubes inserted into a waveguide exposure system. The atria were then irradiated for a period of 30 min, followed by a 30-min recovery period. The control atria were prepared simultaneously and sham exposed. Experiments were conducted at two temperatures, 22 and 37 degrees C, and two specific absorption rates, 2 mW/g and 10 mW/g. At both temperatures the rate of atrial contraction was not altered by a 30-min exposure at either 2 or 10 mW/g. The average rate (beats per min) was approximately 100 for both the control and exposed atria at 22 degrees C and 215 beats per min for both the control and exposed atria at 37 degrees C. In addition, no inotropic effects on the spontaneously beating atria were noted at any exposure level. These data suggest that 2.45-GHz CW microwave radiation at these intensities has no overt effect on these variables in isolated rat atria.