This paper examines the effects that electromagnetic fields from microwave radiation have in enzymatic reactions. Hydrolysis of proteins in beef (
in vivo
case) and casein (
in vitro
case) by the
papain
enzyme, a major industrial enzyme, is used herein as a model reaction to assess, under highly controlled conditions, the various parameters of microwave radiation (electric field, magnetic field, pulsed microwave irradiation, continuous microwave irradiation) as they might influence these
in vivo
and
in vitro
enzymatic reactions. The effect(s) of the microwaves’ electromagnetic fields was clearly evidenced in the
in vivo
case, contrary to the
in vitro
case where no such effect was observed, likely due to the nature of the hydrolysis reaction and to the autolysis (self-digestion) of the papain enzyme. Additionally, the effect of pulsed
versus
continuous microwave irradiation was further assessed by examining the
catalase
-assisted decomposition of hydrogen peroxide.