Microwave processing: A boon for oral pathologists

Kango, Prasad G; Deshmukh, Revati

doi:10.4103/0973-029x.80031

Cited by 10 publications

(23 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is consistent with other studies [12,23]. However, in our study the nuclear details were slightly less clear in microwave fixed tissues.…”

Section: Resultssupporting

confidence: 92%

“…This was similar to the findings of Hopwood D et al, [27] and Emerson LL et al, [32] who showed that malignant cells were equally well preserved by both conventional and microwave fixed tissues. Right from precancerous conditions and lesions to malignancy, reactive lesions to benign tumours, microwave technique has shown a remarkable difference without losing the architecture and morphology of the cells [23].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation

Tripathi

Bansal

Gupta

et al. 2013

JCDR

View full text Add to dashboard Cite

Introduction: Conventional formalin-fixed, paraffin-embedded tissue provides superior cellular morphology and long-term storage. Problems with formalin fixation comprise delay of fixation and variations in the duration of fixation. Microwave assisted tissue fixation removes the use of noxious and potentially toxic formalin that decreases the turnaround time and creates a personnel friendly workflow.

show abstract

“…This is consistent with other studies [12,23]. However, in our study the nuclear details were slightly less clear in microwave fixed tissues.…”

Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation

Tripathi

Bansal

Gupta

et al. 2013

JCDR

View full text Add to dashboard Cite

show abstract

“…Though few authors had utilized chloroform for clearing [5], in the present study, the clearing was done with xylene, with satisfactory results. It was observed that a longer time was needed for the wax impregnation (30 minutes) as compared to that in the previous studies, where it ranged from five minutes to fifteen minutes [14].…”

Section: Discussionmentioning

confidence: 62%

Domestic Microwave Versus Conventional Tissue Processing: A Quantitative and Qualitative Analysis

Pj²,

Bo³

2013

JCDR

View full text Add to dashboard Cite

Background: Microwave irradiation has been tried as a replacement for the conventional tissue processing technique in histopathology laboratories for quite some time. Studies have shown that Domestic Microwave Tissue Processing (DMWTP) provides a faster delivery of the tissue sections with a morphology which is similar to that which is seen Conventional Tissue Processing (CTP). But many laboratories still confine the domestic microwave tissue processing method only to the handle selected specimens, for which urgent reports are needed. One of the probable reasons is that, understanding about the number of tissue sections which can be processed using a microwave oven at a time, with the appropriate quality, still remains unclear. Aim:The aim of this study was to quantitatively analyze the optimum number of samples that a domestic microwave could process at a time, as well as to qualitatively analyze the morphological outcome of those tissue sections with that of conventional processing. Materials and Methods:This study was approved by the research and ethical committee of Sree Balaji Medical College and Hospital. A total of 135 paired tissue sections were included in the study. Ten tissue sections (which are mentioned hereafter as A10) were processed in a domestic microwave and their paired 10 tissues were processed by a conventional method. Subsequently, the number of tissues which was to be processed was increased to B15, C20, D25, E30 and F35, after ascertaining that the morphological qualities of the previously processed tissue sections were satisfactory. Sections of 4 µm thickness were taken and they were stained by the Haematoxylin and Eosin method. The slides of the tissues which were processed by the microwave method and the conventional method were randomly numbered, for a blind study, which were independently evaluated by two observers. The qualities of slides were assessed, based on 4 parameters: the cytoplasmic details, the nuclear details, the tissue architecture and the staining characteristics. The statistical analysis was done by using SPSS 15.0. Results:The morphological outcomes (quality) of the DMWTPs were comparable to that of the CTPs, when the sample load (quantity) in the microwave oven was up to 25 samples.

show abstract

“…The two methods did not show signiicant variation in terms of overall quality, consistent with the observations of numerous authors such as Rohr, Morales, Mathai, Devi, Babu. [1][2][3][4][5][6] Complete concordance of opinion with regard to quality of the two methods was seen in an average of 90.42% cases. Majority of tissue sections were of excellent quality (Score-4).…”

Section: Resultsmentioning

confidence: 81%

“…Total of 200 slides (100 pairs) were evaluated by each observer: O 1 and O 2 . Seven different parameters (1.Cellular clarity, 2.Cytoplasmic details, 3.Nuclear details, 4.Colour intensity, 5 .Interface of epithelium and connective tissue, 6.Fibrous tissue component and 7.Staining quality) were evaluated by two observers and independent scores were given as per obtained quality.…”

Section: Scoringmentioning

confidence: 99%