Microwave radiation can alter protein conformation without bulk heating

Pomerai, David I. de; Smith, Brette; Dawe, Adam; North, Klaus; Smith, Tim; Archer, David B.; Duce, I.R.; Jones, D.; Candido, E. P. M.

doi:10.1016/s0014-5793(03)00413-7

Cited by 197 publications

(141 citation statements)

References 23 publications

Supporting

Mentioning

134

Contrasting

Unclassified

Order By: Relevance

“…There is currently no recognised biophysical basis for such non-thermal effects (Adair, 2002), although there have been suggestions that microwave fields might alter the rates of protein folding and unfolding (Bohr and Bohr, 2000;de Pomerai et al, 2003). This mechanism could plausibly explain the induction of a modest heat-shock response (de Pomerai et al 2000a, b;Leszczynski et al, 2002) via accumulation of partially unfolded proteins within cells.…”

Section: Discussionmentioning

confidence: 99%

A small temperature rise may contribute towards the apparent induction by microwaves of heat-shock gene expression in the nematodeCaenorhabditis Elegans

Dawe

Smith

Thomas

et al. 2006

Bioelectromagnetics

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

A small temperature rise may contribute towards the apparent induction by microwaves of heat-shock gene expression in the nematodeCaenorhabditis Elegans

Dawe

Smith

Thomas

et al. 2006

Bioelectromagnetics

Self Cite

View full text Add to dashboard Cite

“…Based on the experimental results they concluded that MW radiation has the ability to change protein conformation by enhancing the kinetics of protein folding and denaturation, which could eventually lead to detrimental biological effects. De Pomerai et al (25) also showed that aggregation of protein bovine serum albumin could be enhanced by microwave radiation. Other different approaches have been considered regarding the interactions between RF/MW radiation and biological systems.…”

Section: Biological Mechanisms Of Rf/mw Actionmentioning

confidence: 98%

Biological indicators in response to radiofrequency/microwave exposure

Marjanović

Pavičić

Trošić

2012

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

Over the years, due to rapid technological progress, radiation from man-made sources exceeded that of natural origin. There is a general concern regarding a growing number of appliances that use radiofrequency/ microwave (RF/MW) radiation with particular emphasis on mobile communication systems. Since nonthermal biological effects and mechanisms of RF/MW radiation are still uncertain, laboratory studies on animal models, tissues, cells, and cell free system are of extraordinary importance in bioelectromagnetic research. We believe that such investigations play a supporting role in public risk assessment. Cellular systems with the potential for a clear response to RF/MW exposures should be used in those studies. It is known that organism is a complex electrochemical system where processes of oxidation and reduction regularly occur. One of the plausible mechanisms is connected with generation of reactive oxygen species (ROS). Depending on concentration, ROS can have both benefi cial and deleterious effects. Positive effects are connected with cell signalling, defence against infectious agents, and proliferative cell ability. On the other hand, excessive production, which overloads antioxidant defence mechanism, leads to cellular damage with serious potential for disease development. ROS concentration increase within the cell caused by RF/MW radiation seems to be a biologically relevant hypothesis to give clear insight into the RF/MW action at non-thermal level of radiation. In order to better understand the exact mechanism of action and its consequences, further research is needed in the fi eld. We would like to present current knowledge on possible biological mechanisms of RF/MW actions.

show abstract

“…To probe the regulatory role of TF nodes in SRN pathways, we will ablate the function of selected TFs by feeding RNAi (e.g. for HSF-1) [45]. The efficacy of RNAi for each TF will be checked by monitoring reporter expression in TF-promoter::GFP fusion strains.…”

Section: Transcription Factor Nodesmentioning

confidence: 99%

“…The cyp-34A9 promoter contains a direct repeat of the nGAAn motif, but our cyp-34A9::GFP reporter strain shows poor heat inducibility at 35ºC, suggesting that this is not a functional HSF binding site. By contrast, the upstream promoter of cyp-35A2 contains a closer match (AAGCTCTT) to the HSF consensus binding site at around -100; the fact that a cyp-35A2::GFP reporter strain [37] shows strong heat inducibility at 35ºC suggests that this site may well be functional, though RNAi against HSF [45] will be needed to confirm this suggestion.…”

Section: A Case Study: Cyp-34a9 and Daf-16mentioning

confidence: 99%

“…Expression of a daf-16::GFP reporter was reduced by 50% using RNAi against DAF-16, as compared to the feeding vector only (data not shown). Details of the methodology used can be found in de [45]. molecular biologists working on stress-inducible genes in C. elegans or D. melanogaster, to make contact with the authors and to share new findings (e.g.…”

Section: Community Involvement In the Srn Pro-jectmentioning

confidence: 99%

See 1 more Smart Citation

The Stress-Response Network in Animals: Proposals to Develop a Predictive Mathematical Model

Pomerai¹,

Madhamshettiwar²,

Anbalagan³

et al. 2009

TOTOXIJ

View full text Add to dashboard Cite

Abstract:Increasing evidence indicates that numerous genetic pathways responding to environmental stress in animals are regulated co-ordinately as well as independently. These stress-response systems should therefore be viewed in holistic terms as a network. As such, their behaviour is susceptible to mathematical modelling using a systems biology approach. This review outlines relevant evidence and describes a newly launched project to develop just such a model using stressresponse data from multiple transgenic strains of C. elegans and D. melanogaster. We hope that our eventual model will be capable of predicting the effects of simple stressor mixtures with reasonable accuracy. To maximise the effectiveness and scope of this model, we appeal for help from colleagues to share reagents and data relevant to this project. We also present preliminary data where RNA interference has implicated the key transcription factor DAF-16 in an unexpected upregulation of cyp-34A9 reporter expression by high cadmium. STRESS RESPONSES AND MIXTURE TOXICITYIn multicellular organisms, the defensive cellular responses evoked by environmental stresses do not result from simple linear pathways, but rather from a network of interlinked pathways with multiple outputs. This makes it difficult to predict the biological effects of multiple stressors acting together, even though this is the normal situation for industrial pollution of soil or water, where several different contaminants are usually present together. There are few studies and no useful predictive models describing the molecular responses of multicellular organisms to several toxicants acting in concert. This is essentially a systems biology problem, requiring integration of complex molecular and toxicological information. Under the auspices of a Major Award from the UK-India Education and Research Initiative (UK-IERI), we intend to develop an in silico model describing the principal elements of a consensus stress response network (SRN) and its in vivo responses to single stressors, using data from two invertebrate model systems, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster. This model will be used to predict the likely SRN responses to stressor mixtures, and such predictions will then be tested experimentally in both species so that the model can be refined accordingly. Since the SRN core pathways are highly conserved among animal taxa, general features of this model should find wider application in ecotoxi-*Address correspondence to this author at the School of Biology, Nottingham University, Nottingham NG7 2RD, UK; Tel: (0044) 115 951 3250; Fax: (0044) 115 951 3251; E-mail: david.depomerai@nottingham.ac.uk cology. The dynamic aspects of this model, and in particular its ability to integrate multiple toxicant inputs for predicting likely SRN outputs, distinguish this from a simple map of the regulatory gene-circuits comprising the SRN. THE C. ELEGANS STRESS-RESPONSE NETWORKThe free-living soil nematode, C. elegans, is particularly suitable fo...

show abstract

Microwave radiation can alter protein conformation without bulk heating

Cited by 197 publications

References 23 publications

A small temperature rise may contribute towards the apparent induction by microwaves of heat-shock gene expression in the nematodeCaenorhabditis Elegans

A small temperature rise may contribute towards the apparent induction by microwaves of heat-shock gene expression in the nematodeCaenorhabditis Elegans

Biological indicators in response to radiofrequency/microwave exposure

The Stress-Response Network in Animals: Proposals to Develop a Predictive Mathematical Model

Contact Info

Product

Resources

About