We present here a series of Ground Penetrating Radar (GPR) survey carried out in different areas of the Dardanelos 1 archaeological site in order to generate information about subsurface anomalies associated with archaeological material that could be use in decision making within the environmental licensing process of the Dardanelos Hydroelectric Power Plant, located near of the Aripuanã city, Mato Grosso State, northwest region of Brazil. GPR surveys with 200 MHz antenna were carried out in two blocks aiming to locate archaeological resources and features. The analysis of GPR 2D and 3D results allowed detecting anomalous regions characterized by hyperbolic reflections, shallow elongated continuous targets with high amplitudes, as well as sub-horizontal reflectors. Microwave tomography allowed estimating the geometry of the GPR anomalies sources. Excavations were done by archaeologists at the locations where hyperbolic anomalies were found, revealing interesting structures related to urns in the middle of a rich in organic matter consisting of black and ceramic materials up to about 1 m deep. The first sub-horizontal reflector at approximately 1 m depth is related to the base of the black soil layer rich in organic matter and the second sub-horizontal reflector between 2 and 3 m deep suggests a lithological change or may be related to presence of the water table. The continuous elongated shallow targets observed in the depth slices are related to tree roots in the middle of the archaeological strata. The GPR results guided archaeological excavations, reduced the time and costs involved in research, and contributed to the preservation of Brazilian historical heritage.