Optical microscopy
is a valuable tool for in vivo monitoring of biological
structures and functions because of its
noninvasiveness. However, imaging deep into biological tissues is
challenging due to the scattering and absorption of light. Previous
research has shown that the two optimal wavelength windows for high-resolution
deep mouse brain imaging are around 1300 and 1700 nm. However, one-photon
fluorescence imaging in the wavelength region has been highly challenging
due to the poor detection efficiency of currently available detectors.
To fully utilize this wavelength advantage, we demonstrated here one-photon
confocal fluorescence imaging of deep mouse brains with an excitation
wavelength of 1310 nm and an emission wavelength within the 1700 nm
window. Fluorescence emission at 1700 nm was detected by a custom-built
superconducting nanowire single-photon detector (SNSPD) optimized
for detection between 1600 nm and 2000 nm with low detection noise
and high detection efficiency. With the PEGylated quantum dots and
SNSPD both positioned at the optimal imaging window for deep tissue
penetration, we demonstrated in vivo one-photon confocal
fluorescence imaging at approximately 1.7 mm below the surface of
the mouse brain, through the entire cortical column and into the hippocampus
region with a low-cost continuous-wave laser source and low excitation
power. We further discussed the significance of the staining inhomogeneity
in determining the depth limit of one-photon confocal fluorescence
imaging. Our work may motivate the further development of long wavelength
fluorescent probes, and inspire innovations in high-efficiency, high-gain,
and low-noise long wavelength detectors for biological imaging.