Mid-Infrared Spectroscopic Measurement of Ionic Dissociative Materials in the Metabolic Pathway

Nakanishi, Kenichi; Hashimoto, Atsushi; Pan, Tao; Kanou, Mikihito; Kameoka, Takaharu

doi:10.1366/000370203322640152

Cited by 44 publications

(39 citation statements)

References 7 publications

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“…The wave number separation in bipolar peaks of difference spectra greatly exaggerate the actual band shifts and are thus sensitive indicators of small band shifts. These features directly indicate that La 3ϩ ions interacted with negatively charged carboxylate and phosphate groups (22) and exchanged with existing cations in the available binding sites on the bacterial surface. The band at 1,203 cm Ϫ1 is likely due to the PAO stretch in (OOPO 3 La) ϩ , as lanthanum binding resulted in the negative charge no longer being delocalized on multiple oxygen atoms (25).…”

Section: Resultsmentioning

confidence: 98%

Mechanisms of Cation Exchange by Pseudomonas aeruginosa PAO1 and PAO1 wbpL , a Strain with a Truncated Lipopolysaccharide

Shephard

McQuillan

Bremer

2008

Appl Environ Microbiol

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The ability of bacterial cells to sequester cations is well recognized, despite the fact that the specific binding sites and mechanistic details of the process are not well understood. To address these questions, the cationexchange behavior of Pseudomonas aeruginosa PAO1 cells with a truncated lipopolysaccharide (LPS) (PAO1 wbpL) and cells further modified by growth in a magnesium-deficient medium (PAO1 wbpL ؊ Mg 2؉ ) were compared with that of wild-type P. aeruginosa PAO1 cells. P. aeruginosa PAO1 cells had a negative surface charge (zeta potential) between pH 11 and 2.2, due to carboxylate groups present in the B-band LPS. The net charge on PAO1 wbpL cells was increasingly positive below pH 3.5, due to the influence of NH 3 ؉ groups in the core LPS. The zeta potentials of these cells were also measured in Na ؉ , Ca 2؉ , and La 3؉ electrolytes. Cells in the La 3؉ electrolyte had a positive zeta potential at all pH values tested. Growing P. aeruginosa PAO1 wbpL in magnesium-deficient medium (PAO1 wbpL ؊ Mg 2؉ ) resulted in an increase in its zeta potential in the pH range from 3.0 to 6.5. In cation-exchange experiments carried out at neutral pH with either P. aeruginosa PAO1 or PAO1 wbpL, the concentration of bound Ca 2؉ was found to decrease as the pH was reduced from 7.0 to 3.5. At pH 3.5, the bound Mg 2؉ concentration decreased sharply, revealing the activity of surface sites for cation exchange and their pH dependence. Infrared spectroscopy of attached biofilms suggested that carboxylate and phosphomonoester functional groups within the core LPS are involved in cation exchange.The ability of microbial cells to sequester metal ions from aqueous environments is important for microbial growth and biogeochemical processes, such as mineral formation/dissolution, metal transport, and the bioremediation of metal-contaminated sites (1). The external surfaces of gram-negative bacteria are comprised of phospholipids, lipoproteins, lipopolysaccharides (LPS), and proteins. These polymers contain carboxylic acids and phosphate esters, which are primarily responsible for the cells having a net negative charge (at neutral pH), and associated cations, which are retained even after thorough washing with water due to the electroneutrality condition (6, 12, 31). These cations may undergo cation exchange with the introduction of electrolyte solutions containing different cations. While many studies have focused on the amount of metal bound/adsorbed by cells and the development of bulk partitioning models (5), questions such as the mechanism of adsorption (20), the specific binding sites (9), and the impacts of different environmental conditions on adsorption (31) have received less attention. Such information is crucial for the development of models to predict the behavior of metals under a range of conditions and to determine the potential uses of these organisms for environmental remediation (11,15).Pseudomonas aeruginosa is a ubiquitous, well-characterized microorganism used in many metal-binding investigations (10). Suggest...

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Section: Resultsmentioning

confidence: 98%

Mechanisms of Cation Exchange by Pseudomonas aeruginosa PAO1 and PAO1 wbpL , a Strain with a Truncated Lipopolysaccharide

Shephard

McQuillan

Bremer

2008

Appl Environ Microbiol

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“…Furthermore, it is worth noting that the asymmetric phosphate stretching mode originating from the phosphodiester groups of nucleic acids is usually observed at approximately 1240 cm 21 [69]. Therefore, the bands found at this wavenumber in figures 3, 4 and 7 may have a contribution from the asymmetric stretching vibration of phosphodiester groups in nucleic acids.…”

Section: Atr-ir Spectroscopy During Spore Settlement On Tiomentioning

confidence: 88%

“…Lastly, the absorption at 1237 cm 21 must have a contribution from the phosphorylated moieties in chromatin. In fact, the asymmetric phosphate stretching mode originating from the phosphodiester groups of nucleic acids is usually observed at approximately 1240 cm 21 [69]. In addition to the dry spore IR analysis, ATR-IR spectra were collected over 3 h at 148C for a sea water suspension of U. pinnatifida spores covering the Ge prism to a depth of 3 mm, as shown in figure 4.…”

Section: Atr-ir Spectroscopy Of Dry Spores and Spores Settled On Gementioning

confidence: 99%

In situ ATR-IR spectroscopic and electron microscopic analyses of settlement secretions of Undaria pinnatifida kelp spores

Petrone

Easingwood

Barker

et al. 2010

J. R. Soc. Interface.

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Knowledge about the settlement of marine organisms on substrates is important for the development of environmentally benign new methods for control of marine biofouling. The adhesion to substrates by spores of Undaria pinnatifida, a kelp species that is invasive to several countries, was studied by scanning electron and transmission electron microscopies (SEM/TEM) as well as by in situ attenuated total reflection infrared (ATR-IR) spectroscopy. The IR spectra showed that adhesive secretion began approximately 15 min after initial settlement and that the adhesive bulk material contained protein and anionic polysaccharides. Energy dispersive X-ray microanalysis of the adhesive identified sulphur and phosphorus as well as calcium and magnesium ions, which facilitate the gelation of the anionic polysaccharides in the sea water. The adhesive may be secreted from Golgi bodies in the spore, which were imaged by TEM of spore thin sections. Additionally, an in situ settlement study on TiO 2 particle film by ATR-IR spectroscopy revealed the presence of phosphorylated moieties directly binding the substrate. The presence of anionic groups dominating the adhesive suggests that inhibition of spore adhesion will be favoured by negatively charged surfaces.

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“…The pH-dependency of the MIR spectra in aqueous solution of the organic dissociative materials in metabolic pathway, such as saccharide phosphates (G6P, F6P), adenosine and its phosphates (ATP, ADP AMP), was studied [32]. The series of molar absorbance spectra for these reagents were obtained in pH range of about 2 to 11 with the FT-IR/ATR method.…”

Section: Ionic Dissociative Metabolitesmentioning

confidence: 99%

Effective Application of ICT in Food and Agricultural Sector — Optical Sensing is Mainly Described —

Kameoka

Hashimoto

2015

IEICE Trans. Commun.

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SUMMARY This paper gives an outline of key technologies necessary for science-based agriculture. In order to design future agriculture, present agriculture should be redesigned based on the context of smart agriculture that indicates the overall form of agriculture including a social system while the present precision agriculture shows a technical form of agriculture only. Wireless Sensor Network (WSN) and the various type of optical sensors are assumed to be a basic technology of smart agriculture which intends the harmony with the economic development and sustainable agro-ecosystem. In this paper, the current state and development for the optical sensing for environment and plant are introduced.

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Mid-Infrared Spectroscopic Measurement of Ionic Dissociative Materials in the Metabolic Pathway

Cited by 44 publications

References 7 publications

Mechanisms of Cation Exchange by Pseudomonas aeruginosa PAO1 and PAO1 wbpL , a Strain with a Truncated Lipopolysaccharide

Mechanisms of Cation Exchange by Pseudomonas aeruginosa PAO1 and PAO1 wbpL , a Strain with a Truncated Lipopolysaccharide

In situ ATR-IR spectroscopic and electron microscopic analyses of settlement secretions of Undaria pinnatifida kelp spores

Effective Application of ICT in Food and Agricultural Sector — Optical Sensing is Mainly Described —

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