Mid-Membrane Photolabeling of the Transmembrane Domain of Glycophorin A in Phospholipid Vesicles

Ogawa, Yoshikatsu; Hahn, Wolfgang; Garnier, Philippe; Higashi, Nobuaki; Massotte, Dominique; Metz‐Boutigue, Marie‐Hélène; Rousseau, Bernard; Sunamoto, Junzo; Ourisson, Guy; Nakatani, Yôichi

doi:10.1002/1521-3773(20010302)40:5<944::aid-anie944>3.0.co;2-o

Cited by 10 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They observed that most of the cross-linking involved the carboxyl group of Glu 70 , thus suggesting that this residue was located within the bilayer . However, 20 years later the Nakatani group used the same approach with a different lipid probe and obtained very different results. , They used a benzophenone-based bola-phospholipid probe ( 10 , Figure ), thus guaranteeing that the label was located at the center of the bilayer, with no possibility for looping back. DSC characterization of the probe 10 showed complete miscibility with DMPC in the fluid phase .…”

Section: Photolabeling Using Photoreactive Lipidsmentioning

confidence: 99%

Photolabeling Strategies to Study Membranotropic Peptides Interacting with Lipids and Proteins in Membranes

Walrant

Sachon

2021

Bioconjugate Chem.

View full text Add to dashboard Cite

Membranotropic peptides is a class of peptides that exert their biological action at the level of cell membranes. Understanding how they interact with their different membrane binding partners (lipids, proteins and/or glycoconjugates) is important to decipher their mechanism of action. Affinity photolabeling is a powerful method to study non-covalent interactions and provide a sub-molecular picture of the contacts between two interacting partners. In this review, we give a panorama of photolabeling-based studies of the interactions between membranotropic peptides and membranes using either photoreactive lipids or peptides.

show abstract

Section: Photolabeling Using Photoreactive Lipidsmentioning

confidence: 99%

Photolabeling Strategies to Study Membranotropic Peptides Interacting with Lipids and Proteins in Membranes

Walrant

Sachon

2021

Bioconjugate Chem.

View full text Add to dashboard Cite

show abstract

Synthesis and Tritium Labeling of New Aromatic Diazirine Building Blocks for Photoaffinity Labeling and Cross-Linking

et al. 2001

Self Cite

View full text Add to dashboard Cite

show abstract

Synthesis of Carboxyl‐Tethered Symmetric Conjugated Polyenes as Fluorescent Transmembrane Probes of Lipid Bilayers

2003

View full text Add to dashboard Cite

The synthesis of a new series of fluorescent transmembrane probes in which two hydrophilic methyl ester or carboxyl groups are connected by a polymethylene chain, with four, five or six conjugated double bonds in a central position, is reported. The length of the linear structures was designed to match the width of typical lipid bilayers. These bolaamphiphilic compounds result, with overall yields higher than 80%, from an easy PdII‐catalyzed double cross‐coupling between terminal acetylene esters and conjugated 1,ψ‐dihalopolyenes, followed by selective triple bond partial reduction with activated zinc, and iodine isomerization to the all‐(E) isomer. An alternative approach, based on a Stille double cross‐coupling between the appropriate all‐(E)‐ψ‐halopolyenes and (E)‐bis(tributylstannyl)ethene, yielded mixtures that could not be resolved by standard chromatographic methods due to the presence of other simultaneous coupling reactions, which are also discussed in detail. Nevertheless, the Stille method can be of utility for the obtention of carbonyl‐polyene conjugated analogs. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003)

show abstract

Mid-Membrane Photolabeling of the Transmembrane Domain of Glycophorin A in Phospholipid Vesicles

Abstract: The tandem use of the photosensitive bola‐amphiphile 1 (X=3H) and cholesterol enabled the determination of the center of the transmembrane domain of glycophorin A (131 amino acid residues) in a membrane by selective functionalization of the protein within a phospholipid bilayer.

Cited by 10 publications

References 17 publications

Photolabeling Strategies to Study Membranotropic Peptides Interacting with Lipids and Proteins in Membranes

Photolabeling Strategies to Study Membranotropic Peptides Interacting with Lipids and Proteins in Membranes

Synthesis and Tritium Labeling of New Aromatic Diazirine Building Blocks for Photoaffinity Labeling and Cross-Linking

Synthesis of Carboxyl‐Tethered Symmetric Conjugated Polyenes as Fluorescent Transmembrane Probes of Lipid Bilayers

Contact Info

Product

Resources

About