[1] We use the oxygen isotopic composition of planktonic foraminifera Globigerinoides ruber (white) from Ocean Drilling Program Site 1058 in the subtropical northwestern Atlantic to construct a high-resolution ($ 800 year) climate record spanning the mid-Pleistocene climate transition ($410 ka to 1350 ka). We investigate whether or not millennial-scale instabilities in the proxy record are associated with the extent of continental glaciation. G. ruber d 18 O values display high-frequency fluctuations throughout the record, but the amplitude about mean glacial and interglacial d 18 O values increases at marine isotope stage (MIS) 22 (880 ka) and is highest during MIS 12. These observations support that millennial-scale climate instabilities are associated with ice sheet size. Time series analysis illustrates that these variations have significant concentration of spectral power centered on periods of $10-12 ka and $5 ka. The timing of these fluctuations agrees well, or coincides with, the periodicities of the second and fourth harmonics, respectively, of precessional forcing at the equator. An insolation-based origin of the millennial-scale instabilities would be independent of ice volume and explains the presence of these fluctuations before the mid-Pleistocene climate transition as well as during interglacial intervals (e.g., MIS 37 and 17). Because the amplitude of the millennial-scale variations increases during the mid-Pleistocene transition, feedback mechanisms associated with the growth of large, 100-ka-paced, polar ice sheets may be important amplifiers of regional surface water hydrographic changes.