Middle ear fluid characteristics in pediatric otitis media with effusion

Dodson, Kelley M.; Cohen, Randall S.; Rubin, Bruce K.

doi:10.1016/j.ijporl.2012.09.005

Cited by 19 publications

(14 citation statements)

References 19 publications

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“…New increasing evidence suggests the major role of biofilms in the pathogenesis of OME [8][9][10]. Gastroesophageal reflux [11], poor clearance of middle ear fluids [12,13], allergies [14][15][16][17] and finally genetic predisposition [18,19] may also contribute. A bacterial organism is identified in approximately onethird of middle ear effusions of children with OME who undergo myringotomy [20][21][22][23][24] and the pathogens are primarily those that are found in the nasopharyngeal biofilms.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of nasopharyngeal microbial flora and antibiogram and its relation to otitis media with effusion

Nourizadeh

Ghazvini

Gharavi

et al. 2015

Eur Arch Otorhinolaryngol

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Acute otitis media and otitis media with effusion (OME) are the main causes of hearing impairment in children which require proper treatment, mainly antibiotic therapy. Patients whom were appropriate candidates for adenoidectomy were divided into two groups regarding the presence of middle ear effusion. Adenoid tissue specimens were cultured in both groups and the bacterial flora and anti-microbial resistance pattern were determined. 72 patients were studied, 42 % had OME while 58 % did not. The following bacteria were isolated and cultured from both groups with no meaningful difference in prevalence: Streptococcus viridans (p = 0.265), Staphylococcus aureus (p = 0.72), H. influenza (p = 0.806), Entrococcus. spp (0.391), Streptococcus pneumonia (p = 0.391), nonhemolytic Streptococcus (p = 0.230). Bacterial sensitivity was similar for Amoxicillin-clavulanate (p = 0.935), Amoxicillin (p = 0.935), Cephalexin (p = 0.806), Cefixime (p = 0.391) and Azithromycin in both groups. The two groups showed no meaningful difference considering the bacterial flora of nasopharynx and their sensitivity. Bacteria in both groups were sensitive to Amoxicillin and Amoxicillin-clavulanate and resistant to Azithromycin, Cefixime and Cephalexin.

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Section: Resultsmentioning

confidence: 99%

Evaluation of nasopharyngeal microbial flora and antibiogram and its relation to otitis media with effusion

Nourizadeh

Ghazvini

Gharavi

et al. 2015

Eur Arch Otorhinolaryngol

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“…These cells can then form invaginations giving rise to mucus glands that are not found in healthy middle ears 23 . These cells produce an increased amount of mucins, leading to MEEs that are more difficult to clear, 24 which may result in the persistence of MEEs in OME. These mucins appear to play a key role in the innate response to infection, with MUC5B and MUC5AC being the predominantly secreted mucins 25 .…”

Section: The Influence Of Host Response On Ommentioning

confidence: 99%

Review of otitis media microbiome studies: What do they tell us?

Nogues

Pérez‐Losada

Preciado

2020

Laryngoscope Investig Oto

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Objectives: To provide a state of the art review on accruing studies focused on defining the middle ear microbiome, highlighting the relationship of the microbiome to disease pathophysiology. Data sources: Pubmed indexed peer-reviewed articles and published textbooks. Review methods: Comprehensive review of the literature using the following search terms: "microbiome" "bacterial pathogens" with the term "otitis media," and "middle ear." Results: A multitude of microbiome studies have been published in the recent past. In general findings from these studies underscore distinct profiles based on disease category. The adenoidal reservoir theory may not explain all etiologies of middle ear effusion production. The host immune system appears to be associated to the bacterial population identified in the middle ear space. Atopic respiratory diseases correlate to the middle ear microbiome. Some novel middle ear bacterial genera may be protective in terms of disease. Conclusion: The understanding of otitis media disease progression pathophysiology is evolving, informed by accruing middle ear microbiomic data. The functional implications of middle ear microbiome findings need to be studied further. This may help counterbalance probiotic vs antibiotic approaches to disease mitigation.

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“…Less frequently, COME MEEs are not as viscous and are characterized as serous. Although, the biologic reasons for this are mostly unknown, studies have linked serous effusions to better medical outcomes in patients relative to those with mucoid effusions ( 16 , 17 ). Matkovic et al ( 18 ) linked the viscosity characteristics of MEEs to the duration of OM: among the 108 samples they collected, 6% of the effusions were mucoid for patients having OM less than 3 months, but 95% were found mucoid after 3 months of OM diagnosed.…”

Section: Introductionmentioning

confidence: 99%

Characterization of mucoid and serous middle ear effusions from patients with chronic otitis media: implication of different biological mechanisms?

et al. 2018

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BackgroundChronic Otitis Media with effusion (COME) is characterized by persistent middle ear effusions that are in most cases highly viscous, but some patients present with serous fluid. This study aimed at comprehensively characterizing the macromolecular composition of mucoid vs. serous MEEs.MethodsMEEs from patients with COME were analyzed for proteins by Mass Spectrometry (MS) and western blot techniques, total DNA quantity, bacterial DNA (16S sequencing) and cytokine content. Proteomics datasets were studied in Ingenuity pathway analysis (IPA).ResultsMucoid samples showed a global tendency of increased pro- inflammatory mediators. IL1-β and IL-10 were significantly more abundant in serous samples (p<0.01). Mucoid samples had higher DNA quantity (p=0.04), more likely to be positive in MUC5B protein (p=0.008) and higher peptide counts (12786 vs 2225), as well as an overall larger number of identified proteins (331 vs 177), compared to serous. IPA found the mucoid sample dataset to be related to immune cell function and epithelial remodeling whereas the serous sample dataset showed acute responses and blood related proteins. Interestingly, serous samples showed more bacterial DNA than mucoid ones, with less bacterial genera variability.ConclusionThis study demonstrates divergent immune responses in children with COME by effusion quality.

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Middle ear fluid characteristics in pediatric otitis media with effusion

Cited by 19 publications

References 19 publications

Evaluation of nasopharyngeal microbial flora and antibiogram and its relation to otitis media with effusion

Evaluation of nasopharyngeal microbial flora and antibiogram and its relation to otitis media with effusion

Review of otitis media microbiome studies: What do they tell us?

Characterization of mucoid and serous middle ear effusions from patients with chronic otitis media: implication of different biological mechanisms?

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