Midterm Outcomes of Autologous Cultivated Limbal Stem Cell Transplantation With or Without Penetrating Keratoplasty

Baradaran‐Rafii, Alireza; Ebrahimi, Marzieh; Kanavi, Mozhgan Rezaei; Taghiabadi, Ehsan; Aghdami, Nasser; Eslani, Medi; Bakhtiari, Pejman; Einollahi, Bahram; Baharvand, Hossein; Javadi, Mohammad-Ali

doi:10.1097/ico.0b013e3181bd9f60

Cited by 71 publications

(42 citation statements)

References 34 publications

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“…This review includes 28 case reports and series published over the past 13 years [Pellegrini et al, 1997;Schwab, 1999;Schwab et al, 2000;Tsai et al, 2000;Rama et al, 2001Rama et al, , 2010Koizumi et al, 2001a,b;Grueterich et al, 2002;Nakamura et al, 2003Nakamura et al, , 2004Nakamura et al, , 2006Sangwan et al, 2003Sangwan et al, , 2005Daya et al, 2005;Ang et al, 2007;Fatima et al, 2007;Kawashima et al, 2007;Shimazaki et al, 2007;Shortt et al, 2008;Di Girolamo et al, 2009;Meller et al, 2009;Baradaran-Rafii et al, 2010;Colabelli Gisoldi et al, 2010;Di Iorio et al, 2010;Kolli et al, 2010;Pauklin et al, 2010]. There were 10 studies with 5 patients or less, 13 studies with 6-20 patients, and 5 with more than 20 patients.…”

Section: General Analysis Of the Studiesmentioning

confidence: 95%

“…Daya et al [2005] performed punctal occlusion in all cases prior to limbal stem cell transplantation to aid ocular lubrication. Three studies described complications of surgery related to exposure or lid deformities that had not been corrected preoperatively [Baradaran-Rafii et al, 2010;Koizumi et al, 2001a;Pauklin et al, 2010]. Meller et al [2009] did not perform cultured limbal epithelial transplantation initially due to dry eye in one patient but it was performed later as this parameter improved through systemic therapy.…”

Section: Assessment Of Adnexa and Dry Eyesmentioning

confidence: 97%

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13 years of cultured limbal epithelial cell therapy: A review of the outcomes

Baylis

Figueiredo

Henein

et al. 2011

J of Cellular Biochemistry

227

219

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The cornea is the clear tissue at the front of the eye which enables the transmission of light to the retina for normal vision. The surface of the cornea is composed of an epithelium which is renewed by stem cells located at the periphery of the cornea, a region known as the limbus. These limbal stem cells can become deficient as a result of various diseases of the eye's surface, resulting in the blinding disease of limbal stem cell deficiency. The treatment of this disease is often difficult and complex. In 1997, it was proposed that a small amount of limbal tissue containing limbal stem cells could be culture expanded and then transplanted. Since then various case reports and case series have been reported showing promising results. Here, we review the outcomes of this procedure over the past 13 years with the aim of highlighting the best culture and surgical techniques to date.

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Section: General Analysis Of the Studiesmentioning

confidence: 95%

Section: Assessment Of Adnexa and Dry Eyesmentioning

confidence: 97%

13 years of cultured limbal epithelial cell therapy: A review of the outcomes

Baylis

Figueiredo

Henein

et al. 2011

J of Cellular Biochemistry

227

219

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“…Over the past decade, cultured limbal epithelial stem cell transplantation (CLET) has been widely accepted as a standard approach for the ocular surface reconstruction of patients with LSCD. (Pellegrini et al 1997 ;Schwab et al 2000 ;Tsai et al 2000a , b ;Sangwan et al 2005 ;Baradaran-Rafi i et al 2010 ;Kolli et al 2010 ;Mariappan et al 2010 ). Cultured limbal epithelial transplantation (CLET) followed by penetrating keratoplasty (PKP) has resulted in long term graft survival and improved visual outcome (Sangwan et al 2005 ) (Fig.…”

Section: Clinical Principles Diagnostics Indications Of Ocular Surfmentioning

confidence: 97%

Regenerative Therapies for the Ocular Surface

Vemuganti¹,

Sangwan²,

Mariappan³

et al. 2016

Regenerative Medicine - From Protocol to Patient

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Integrity of ocular surface depends on adequate tear fi lm and stability of the surface epithelium consisting of two specialized phenotypically different epithelial cells, the central transparent corneal epithelium and the peripheral conjunctival cells, separated by a more specialized transition zone, called the limbus. Similar to the epithelial regeneration in other parts of the body, the corneal epithelium is regenerated from the stem cells located in limbus. Severe chemical burns and other diseases can cause damage to the limbus, resulting in a condition called Limbal Stem Cell Defi ciency (LSCD). Effective therapeutic modalities for this visionthreatening condition include use of human amniotic membrane, replenishing the stores of limbal stem cells by limbal transplantation. However last decade has witnessed the use of ex-vivo expanded sheet of limbal epithelial cells for ocular surface reconstruction in such cases. Our group has established a simple, feeder-cell free, cost-effective way of culturing the corneal epithelium from limbal tissues within 2 weeks, using human amniotic membrane as a vehicle. The interim results of a clinical trial involving 700 patients with severe unilateral and bilateral LSCD revealed

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“…[1][2][3]4 In unilateral total LSCD, conjunctival limbal autograft (CLAU) taken from the healthy fellow eye with or without amniotic membrane transplantation (AMT) is one of the alternatives. [1][2][3][4][5] There are several case series in the literature about CLAU demonstrating successful anatomical results and visual outcomes of the surgery. [6][7][8][9][10][11][12][13][14] To the best of our knowledge, reported literature regarding complications of this surgery is limited and sparse.…”

mentioning

confidence: 99%

Postoperative Complications of Conjunctival Limbal Autograft Surgery

Baradaran‐Rafii

Eslani

Jamali

et al. 2012

Cornea

Self Cite

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Conjunctival limbal autograft is an effective procedure for anatomical and visual rehabilitation of eyes with unilateral total LSCD. However, it may be complicated by several adverse events. The most common complications are related to the transplanted grafts (size, thickness, position, and alignment) and chronic ocular surface exposure, which may later lead to epithelial breakdown and corneal conjunctivalization.

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Midterm Outcomes of Autologous Cultivated Limbal Stem Cell Transplantation With or Without Penetrating Keratoplasty

Cited by 71 publications

References 34 publications

13 years of cultured limbal epithelial cell therapy: A review of the outcomes

13 years of cultured limbal epithelial cell therapy: A review of the outcomes

Regenerative Therapies for the Ocular Surface

Postoperative Complications of Conjunctival Limbal Autograft Surgery

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