MIG1-dependent and MIG1-independent glucose regulation of MAL gene expression in Saccharomyces cerevisiae

Hu, Zhen; Nehlin, Jan O.; Ronne, Hans; Michels, Corinne A.

doi:10.1007/bf00309785

Cited by 91 publications

(84 citation statements)

References 33 publications

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“…This glucose signaling results in a rapid but transient spike in the level of intracellular cAMP, which increases 5-to 50-fold within 1 to 2 min of glucose addition and returns to near-basal levels within 20 min. When cells express the nonpalmitoylated cytoplasmic Ras2 C318S form as their only Ras protein, they fail to exhibit the glucose-induced cAMP spike and are defective in transitioning to rapid growth upon addition of glucose (160). Thus, glucose-regulated cAMP production appears to require attachment of Ras to the yeast plasma membrane.…”

Section: Early Steps In Signal Transductionmentioning

confidence: 99%

Glucose Signaling in Saccharomyces cerevisiae

Santangelo

2006

Microbiol Mol Biol Rev

481

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SUMMARY Eukaryotic cells possess an exquisitely interwoven and fine-tuned series of signal transduction mechanisms with which to sense and respond to the ubiquitous fermentable carbon source glucose. The budding yeast Saccharomyces cerevisiae has proven to be a fertile model system with which to identify glucose signaling factors, determine the relevant functional and physical interrelationships, and characterize the corresponding metabolic, transcriptomic, and proteomic readouts. The early events in glucose signaling appear to require both extracellular sensing by transmembrane proteins and intracellular sensing by G proteins. Intermediate steps involve cAMP-dependent stimulation of protein kinase A (PKA) as well as one or more redundant PKA-independent pathways. The final steps are mediated by a relatively small collection of transcriptional regulators that collaborate closely to maximize the cellular rates of energy generation and growth. Understanding the nuclear events in this process may necessitate the further elaboration of a new model for eukaryotic gene regulation, called “reverse recruitment.” An essential feature of this idea is that fine-structure mapping of nuclear architecture will be required to understand the reception of regulatory signals that emanate from the plasma membrane and cytoplasm. Completion of this task should result in a much improved understanding of eukaryotic growth, differentiation, and carcinogenesis.

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Section: Early Steps In Signal Transductionmentioning

confidence: 99%

Glucose Signaling in Saccharomyces cerevisiae

Santangelo

2006

Microbiol Mol Biol Rev

481

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“…It remains to be determined whether repressors, such as that encoded by MIG1 26,33,63 , are ineffective because the AGT1 promoter sequences through which they act are altered or absent. Certainly, expression patterns of MALx3 and AGT1 are not closely matched (Fig.…”

Section: (-7'977-32mentioning

confidence: 99%

Expression Patterns of Genes and Enzymes Involved in Sugar Catabolism in IndustrialSaccharomyces cerevisiaeStrains Displaying Novel Fermentation Characteristics

Meneses

Jiranek

2002

Journal of the Institute of Brewing

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The utilisation of maltose or sucrose by a selection of nine brewing, baking or laboratory strains of Saccharomyces cerevisiae was either repressible, constitutive or absent. Overall fermentation rate showed a good correlation with maximum specific maltose transport rate (R 2 = 0.79), but a poor correlation with maximum maltase activity (R 2 = 0.34), implying that transport rather than hydrolysis of maltose was a rate-limiting step determining fermentation performance. The genetic basis for differences seen between the strains in terms of fermentation kinetics was investigated. All strains were found to possess genomic sequences detectable with probes for the MAL11 (AGT1), MAL31 permease genes and the SUC2 invertase gene, however, the loci present and the pattern of expression of these and other MAL genes varied widely. From comparisons of sugar utilisation and gene expression patterns, constitutive maltose utilisation in strain NCYC 1681 was best explained by AGT1 expression. Such expression of AGT1 was not, however, mirrored by changes in the pattern of expression of the transcriptional activator(s) (MALx3). Patterns of sucrose utilisation were poorly predicted by the kinetics of SUC2 gene expression, indicating that other SUC loci may be of greater importance.

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“…Preference for glucose is in part due to repression of transcription of genes not required for growth on glucose, such as genes encoding enzymes for converting other carbon sources to glycolytic intermediates (e.g., GAL and SUC), gluconeogenesis (e.g., FBP1 and PCK1), and respiration (e.g., CYCI and COX6) (for reviews, see Johnston and Carlson, 1992;Trumbly, 1992;Ronne, 1995). Migl is the repressor responsible for glucose repression of many genes, including GAL, SUC, and MAL (Nehlin and Ronne, 1990;Griggs and Johnston, 1991;Nehlin et al, 1991;Schuller and Entian, 1991;Flick and Johnston, 1992;Johnston et al, 1994;Vallier and Carlson, 1994;Hu et al, 1995;Lutfiyya and Johnston, 1996;Wang and Needleman, 1996). Migl is a Cys2-His2 zinc finger-containing protein that binds to promoters of glucose-repressed genes and recruits the general repressors Ssn6 and Tupl Struhl, 1994, 1995;Treitel and Carlson, 1995).…”

Section: Introductionmentioning

confidence: 99%

Regulated nuclear translocation of the Mig1 glucose repressor.

Vít

Waddle

Johnston

1997

MBoC

321

182

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Glucose represses the transcription of many genes in bakers yeast (Saccharomyces cerevisiae). Mig1 is a Cys2-His2 zinc finger protein that mediates glucose repression of several genes by binding to their promoters and recruiting the general repression complex Ssn6-Tup1. We have found that the subcellular localization of Mig1 is regulated by glucose. Mig1 is imported into the nucleus within minutes after the addition of glucose and is just as rapidly transported back to the cytoplasm when glucose is removed. This regulated nuclear localization requires components of the glucose repression signal transduction pathway. An internal region of the protein separate from the DNA binding and repression domains is necessary and sufficient for glucose-regulated nuclear import and export. Changes in the phosphorylation status of Mig1 are coincident with the changes in its localization, suggesting a possible regulatory role for phosphorylation. Our results suggest that a glucose-regulated nuclear import and/or export mechanism controls the activity of Mig1.

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MIG1-dependent and MIG1-independent glucose regulation of MAL gene expression in Saccharomyces cerevisiae

Cited by 91 publications

References 33 publications

Glucose Signaling in Saccharomyces cerevisiae

Glucose Signaling in Saccharomyces cerevisiae

Expression Patterns of Genes and Enzymes Involved in Sugar Catabolism in IndustrialSaccharomyces cerevisiaeStrains Displaying Novel Fermentation Characteristics

Regulated nuclear translocation of the Mig1 glucose repressor.

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