Water used for water supply undergoes numerous changes that affect its composition prior to entering the water supply system (WSS). Once it enters the WSS, it is subject to numerous influences altering its physical and chemical composition, redox potential, and microbial quality. Observations of water quality parameters at different locations within the WSS indicate that it is justified to assume that these processes take place from the source to the end user. In this study, we used the results of routine everyday analyses (EC, T, pH, ORP, chloride, nitrate, nitrite, ammonium, and bacteria) supplemented by experimental data from a one-year sampling campaign assessing the main cations and anions and stable isotopes δ2H and δ18O. Through these data, the statistical significance of the differences between the concentrations of the basic water quality parameters among different WSS locations was determined, together with the water retention time in the system. The results indicate minor changes in water chemical composition within the observed WSS, remaining below the prescribed Maximum Contaminant Level (MCL) for human consumption. However, factors such as water retention time, CaCO3 deposition, pH fluctuations, and bacterial growth may influence its suitability, which necessitates further investigation into potential risks affecting water quality.