Migration of fish leucocytes in vitro: The effect of factors which may be involved in mediating inflammation

Nash, K.A.; Fletcher, Thelma C.; Thomson, A. W.

doi:10.1016/0165-2427(86)90113-3

Cited by 32 publications

(14 citation statements)

References 13 publications

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“…At the day 1 postinoculation of BCG, the process was characterized as a diffuse inflammatory response with the mononuclear infiltrates, interstitial edema and the sites of tissue necrosis, which agree with the study by Finn and Nielsen (1971). The intense peri-vascular inflammatory response observed in other studies (Finn and Nielsen, 1971;Nash et al, 1986;Matushima, 1994) was also observed in this study and was indicative of the macrophage migration towards the lesion site. During the experiment, the morphological alterations were observed in the phagocytic cells, such as differentiation into the multinuclear giant cells and this process was time-dependent.…”

Section: Discussionsupporting

confidence: 81%

Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG

Sado

Matushima

2008

Braz. arch. biol. technol.

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Section: Discussionsupporting

confidence: 81%

Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG

Sado

Matushima

2008

Braz. arch. biol. technol.

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“…Weeks et al (1986) and MacArthur et al (1985) have described similar migration responses in fish to bacterial antigens. Previous studies of cellular migration in fish have been chiefly concerned with responses to known mammalian chemo-attractants (Griffin, 1984; Obenauf & Hyder Smith, 1985; Hunt & Rowley, 1986;Nash et al, 1986;Suzuki, 1986;Howell, 1987). The peak ofcellular response in mullet infected with C. lingua is relatively short-lived and precedes the production of antibody.…”

Section: Discussionmentioning

confidence: 99%

The immune response of the thick‐lipped grey mullet, Chelon labrosus (Risso, 1826), to metacercarial infections of Cryptocotyle lingua (Creplin, 1825)

Wood

Matthews

1987

Journal of Fish Biology

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Grey mullet, Chelon labrosur, (60-IOOg) have been found to respond immunologically to Crypfocofyle lingua, following a single exposure to 20000 cercariae, by the production of humoral antibody, sensitized pronephric leucocytes and cytotoxic serum factors. Antibody titres measured by passive haemagglutination reached a peak at week 4 with a -log, titre of 16 f SE. 1 .O, and titres of 10.7 f S.E I .O were still recorded after 10 weeks at the termination of the experiment. Cercarial agglutination was found unreliable as a rapid test. Pronephric leucocytes, sensitive to cercarial antigen when measured by the under-agarose migration method, were detected between weeks 1 and 6, peaking at week 2. In v i m polarization was increased when cells were incubated with the antigen, but this increase was not significantly different between control and infected fish. Heat-labile cytotoxic factors of immune sera have been demonstrated to whole cercariae in vifro, these factors being associated with structural damage to the tegument. Pronephros cells isolated from immune fish during each week of infection showed no evidence of adherence to cercariae or metacercariae in vitro. The results are discussed in relation to both host response and course of infection of the parasite.

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“…1983/ 1984), b u t similar experimental procedures are being developed in fish (Nash et al, 1986), and development of such techniques will greatly increase our understanding of the inflammatory response in eels to acute bacterial infection.…”

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confidence: 98%

Characterization of inflammatory neutrophils induced by bacterial endotoxin in the blood of eels, Anguilla australis

Hine

Wain

1988

Journal of Fish Biology

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Peripheral blood neutrophils were studied in eels injected with phosphate bulkred saline, bacterial lipopolysaccharidc (LPS), carrageenan (CAR). LPS and CAR together (CAR/LPS(T)), CAR and LPS 4 days later (CAR/LPS(S)), zymosan and latex beads. All stimuli caused a left shift toward ncutrophil immaturity but LPS-elicited neutrophils. particularly in CAR/LPS stimulated eels, were often extremely bdsophik. enlarged, vacuolated and had a large nucleus with poorly differentiated nuclear chromatin. These intensely basophilic neutrophils (IBN) had few granules and lower acid phosphatase and non-specific esterase, but higher PAS levels, compared to normal neutrophils. Ultrastructurally. IBN showed loss of cytoplasmic content that gave a ragged appearance. the nucleus was often immature and many rough cndoplasmic reticulum profiles restricted the distribution of reduced numbers of cytoplasmic granules. These features are attributed to asynchronous development. and IBN are compared with mammalian toxic neutrophils.

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Migration of fish leucocytes in vitro: The effect of factors which may be involved in mediating inflammation

Cited by 32 publications

References 13 publications

Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG

Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG

The immune response of the thick‐lipped grey mullet, Chelon labrosus (Risso, 1826), to metacercarial infections of Cryptocotyle lingua (Creplin, 1825)

Characterization of inflammatory neutrophils induced by bacterial endotoxin in the blood of eels, Anguilla australis

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