Migration of human mesenchymal stem cells stimulated with pulsed
electric field and the dynamics of the cell surface glycosylation

Jezierska-Woźniak, Katarzyna; Lipiński, Seweryn; Huflejt, Margaret E.; Grabarczyk, Łukasz; Barczewska, Monika; Habich, Aleksandra; Wojtkiewicz, Joanna; Maksymowicz, Wojciech

doi:10.17219/acem/90872

Cited by 3 publications

(4 citation statements)

References 36 publications

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“…Previous in vitro experiments that exposed cells and/or scaffolds to EStim, demonstrated its ability to influence cell functions associated with enhanced bone healing [7][8][9][10][11][12][13][14]. These experiments were conducted on a variety of different cell types; bone marrow-derived mesenchymal stem cells (BM-MSC), from human and animal origin [10,11,[15][16][17][18][19][20][21][22][23][24][25]; adipose-derived mesenchymal stem cells (AT-MSC) [11,20,[26][27][28][29][30], mouse osteoblast-like cells [31][32][33] and more recently, human dental pulp stem cells (DPSC) [34]. The above cell types are commonly studied for use in BTE applications.…”

Section: Estim's Effects On Cell Functionmentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

162

148

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Electrical stimulation (EStim) has been shown to promote bone healing and regeneration both in animal experiments and clinical treatments. Therefore, incorporating EStim into promising new bone tissue engineering (BTE) therapies is a logical next step. The goal of current BTE research is to develop combinations of cells, scaffolds, and chemical and physical stimuli that optimize treatment outcomes. Recent studies demonstrating EStim's positive osteogenic effects at the cellular and molecular level provide intriguing clues to the underlying mechanisms by which it promotes bone healing. In this review, we discuss results of recent in vitro and in vivo research focused on using EStim to promote bone healing and regeneration and consider possible strategies for its application to improve outcomes in BTE treatments. Technical aspects of exposing cells and tissues to EStim in in vitro and in vivo model systems are also discussed.

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Section: Estim's Effects On Cell Functionmentioning

confidence: 99%

Electrical stimulation in bone tissue engineering treatments

Leppik

Oliveira

Bhavsar

et al. 2020

Eur J Trauma Emerg Surg

162

148

View full text Add to dashboard Cite

show abstract

“…mASCs exhibited a positive correlation between cell migration rate and ES intensity (from 1 V/cm to 10 V/cm) [89]. The same performance showed in human BMSCs, as the ES intensity is above the physiological level, the migration rate increases while physiological levels of ES do not affect cell motility, this behavior may be essential for maintaining transplanted cells at the lesion site [88, 105]. Different research groups have conflict results in the same cell type.…”

Section: Introductionmentioning

confidence: 97%

“…The ES intensity can stimulate cell migration from a minimum of 0.1 V/cm to a maximum of 12 V/cm and did not cause any significant damage to cells, did not affect cell phenotype or the differentiation potential [101–103]. At the same time, cells showed gradually increased migration rate and distance with higher ES strength [101, 104, 105].…”

Section: Introductionmentioning

confidence: 99%

Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering

et al. 2019

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Recently, electrical stimulation as a physical stimulus draws lots of attention. It shows great potential in disease treatment, wound healing, and mechanism study because of significant experimental performance. Electrical stimulation can activate many intracellular signaling pathways, and influence intracellular microenvironment, as a result, affect cell migration, cell proliferation, and cell differentiation. Electrical stimulation is using in tissue engineering as a novel type of tool in regeneration medicine. Besides, with the advantages of biocompatible conductive materials coming into view, the combination of electrical stimulation with suitable tissue engineered scaffolds can well combine the benefits of both and is ideal for the field of regenerative medicine. In this review, we summarize the various materials and latest technologies to deliver electrical stimulation. The influences of electrical stimulation on cell alignment, migration and its underlying mechanisms are discussed. Then the effect of electrical stimulation on cell proliferation and differentiation are also discussed.

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“…Electric fields have also been observed to affect the migration of cells, – differentiation, , and increase of growth factor and DNA syntheses . Cell migration is a complex process that involves the coordinated movement of cells in response to various signals, such as chemical gradients, mechanical cues, and electrical fields. , One of the main mechanisms by which μsPEFs affect cell migration is through the modulation of the cytoskeletal dynamics.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Cell Viability and Migration of Primary Bovine Annular Fibrosus Fibroblast-like Cells Induced by Microsecond Pulsed Electric Field Exposure

Atsu,

Mowen,

Thompson

2023

ACS Omega

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This study is the first to report the enhancement of cell migration and proliferation induced by in vitro microsecond pulsed electric field (μsPEF) exposure of primary bovine annulus fibrosus (AF) fibroblast-like cells. AF primary cells isolated from fresh bovine intervertebral disks (IVDs) are exposed to 10 and 100 μsPEFs with different numbers of pulses and applied electric field strengths. The results indicate that 10 μs-duration pulses induce reversible electroporation, while 100 μs pulses induce irreversible electroporation of the cells. Additionally, μsPEF exposure increased AF cell proliferation up to 150% while increasing the average migration speed by 0.08 μm/min over 24 h. The findings suggest that the effects of PEF exposure on cells are multifactorial� depending on the duration, intensity, and number of pulses used in the stimulation. This highlights the importance of optimizing the μsPEF parameters for specific cell types and applications. For instance, if the goal is to induce cell death for cancer treatment, then high numbers of pulses can be used to maximize the lethal effects. On the other hand, if the goal is to enhance cell proliferation, a combination of the number of pulses and the applied electric field strength can be tuned to achieve the desired outcome. The information gleaned from this study can be applied in the future to in vitro cell culture expansion and tissue regeneration.

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Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

Abstract: We found the described approach convenient for investigations and for the in vitro modeling of the cellular systems intended for the regenerative cell transplantations in vivo. Probing cell surface glycomes may provide valuable biomarkers to assess the competence of transplanted cells.

Cited by 3 publications

References 36 publications

Electrical stimulation in bone tissue engineering treatments

Electrical stimulation in bone tissue engineering treatments

Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering

Enhanced Cell Viability and Migration of Primary Bovine Annular Fibrosus Fibroblast-like Cells Induced by Microsecond Pulsed Electric Field Exposure

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