Migration of Iron Oxide–labeled Human Hematopoietic Progenitor Cells in a Mouse Model: In Vivo Monitoring with 1.5-T MR Imaging Equipment

Daldrup-Link, Heike E.; Rudelius, Martina; Piontek, Guido; Metz, Stephan; Bräuer, Rosalinde; Debus, Gerlinde; Corot, Claire; Schlegel, Jürgen; Link, Thomas M.; Peschel, Christian; Rummeny, Ernst J.; Oostendorp, Robert A.J.

doi:10.1148/radiol.2341031236

Cited by 169 publications

(151 citation statements)

References 31 publications

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“…The internalized iron oxide particles are subsequently in part metabolized, randomely distributed between dividing cells or released by dying cells (21). Thus, our cell tracking method provides only accurate information about homing of the 14 labeled cells in the target tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Currently available cell labeling techniques are based on radioactive, fluorescent or (super-)paramagnetic markers (14)(15)(16)(17)(18)(19)(20)(21). Labeling techniques with radioactive markers provide a high sensitivity but limited spatial resolution and the risk of radiotoxic cell damage (14)(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

“…However, MR cell tracking techniques are restricted by a limited sensitivity, which requires highly efficient labeling techniques in order to load up the investigated cells with high amounts of contrast agent particles. Such optimized labeling methods have been developed before for various hematopoietic cell populations, including lymphocytes, monocytes and hematopoietc progenitor cells (17,(20)(21)(22)(23)(24)(25)(26). For non-phagocytic cell types, such labeling techniques required transfection, i.e.…”

Section: Introductionmentioning

confidence: 99%

“…For non-phagocytic cell types, such labeling techniques required transfection, i.e. internalization of contrast agents into the cells with the help of liposomes, proteins or viral vectors (17,(20)(21)(22)(23)(24)(25). Genetically engineered lymphocytes already underwent a transfection process in order to shuttle new genetic information into the cells.…”

Section: Introductionmentioning

confidence: 99%

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In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

Daldrup-Link

Meier

Rudelius³

et al. 2004

Eur Radiol

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147

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

Daldrup-Link

Meier

Rudelius³

et al. 2004

Eur Radiol

Self Cite

159

147

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“…In the study by Boss et al [3], the intraportal injection of MSCs is attractive because cells distribute throughout the organ following a single injection. Alternatively, hepatic MR imaging following intravenously injection of iron oxide-labeled human hematopoietic progenitor cells has recently be reported in mice [5]. Moreover, following intravenous injection in rats, an external magnet placed near the liver can attract ironoxide-labeled MSCs into the liver [6].…”

mentioning

confidence: 99%

Tracking mesenchymal stem cells in the liver by magnetic resonance imaging

Pastor

2005

Journal of Hepatology

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In vivo MR imaging of intravascularly injected magnetically labeled mesenchymal stem cells in rat kidney and liver. Bos C, Delmas Y, Desmouliere A, Solanilla A, Hauger O, Grosset C, Dubus I, Ivanovic Z, Rosenbaum J, Charbord P, Combe C, Bulte JW, Moonen CT, Ripoche J, Grenier N.Purpose: To evaluate in vivo magnetic resonance (MR) imaging with a conventional 1.5-T system for depiction and tracking of intravascularly injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs).Materials and Methods: This study was conducted in accordance with French law governing animal research and met guidelines for animal care and use. Rat MSCs were labeled with SPIO and transfection agent. Relaxation rates at 1.5 T, cell viability, proliferation, differentiation capacity, and labeling stability were assessed in vitro as a function of SPIO concentration. MSCs were injected into renal arteries of healthy rats (labeled cells in four, unlabeled cells in two) and portal veins of rats treated with carbon tetrachloride to induce centrolobular liver necrosis (labeled cells and unlabeled cells in two each). Follow-up serial T2*-weighted gradient-echo MR imaging and R2* mapping were performed. MR imaging findings were compared histologically.Results: SPIO labeling caused a strong R2* effect that increased linearly with iron dose; R2* increase for cells labeled for 48 h with 50 mg of iron per milliliter was 50 sec (K1) per million cells per milliliter. R2* was proportional to iron load of cells. SPIO labeling did not affect cell viability (PO27). Labeled cells were able to differentiate into adipocytes and osteocytes. Proliferation was substantially limited for MSCs labeled with 100 mg Fe/mL or greater. Label half-life was longer than 11 days. In normal kidneys, labeled MSCs caused signal intensity loss in renal cortex. After labeled MSC injection, diseased liver had diffuse granular appearance. Cells were detected for up to 7 days in kidney and 12 days in liver. Signal intensity loss and fading over time were confirmed with serial R2* mapping. At histologic analysis, signal intensity loss correlated with iron-loaded cells, primarily in renal glomeruli and hepatic sinusoids; immunohistochemical analysis results confirmed these cells were MSCs.Conclusions: MR imaging can aid in monitoring of intravascularly administered SPIO-labeled MSCs in vivo in kidney and liver.[ . The need for high concentrations of contrast agents and high ionizing radiation preclude the use of computed tomography for such tracking. Bioluminescence utilizes light generated by the enzyme luciferase that has high absorption and scatters in living tissues. Moreover, this technique requires the stable expression of nonhuman genes. Fluorescence (another optical imaging) uses exogenous fluorophores that have high photon absorption and scatter that limit imaging to near-surface organs. Single photon emission computed tomography and positron emission tomography have also been used with different methods: direct loading of a radiometal, enzymatic conver...

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Cellular Magnetic Labeling with Iron Oxide Nanoparticles

Boutry

Laurent

Elst

et al. 2011

Nanoplatform‐Based Molecular Imaging

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Migration of Iron Oxide–labeled Human Hematopoietic Progenitor Cells in a Mouse Model: In Vivo Monitoring with 1.5-T MR Imaging Equipment

Abstract: The in vivo distribution of intravenously administered iron oxide-labeled hematopoietic progenitor cells can be monitored with 1.5-T MR imaging equipment.

Cited by 169 publications

References 31 publications

In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

In vivo tracking of genetically engineered, anti-HER2/neu directed natural killer cells to HER2/neu positive mammary tumors with magnetic resonance imaging

Tracking mesenchymal stem cells in the liver by magnetic resonance imaging

Cellular Magnetic Labeling with Iron Oxide Nanoparticles

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