Migration of Salmonella enteritidis from the albumen into the egg yolk

Braun, Peggy G.; Fehlhaber, K.

doi:10.1016/0168-1605(94)00081-g

Cited by 54 publications

(29 citation statements)

References 5 publications

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“…Strains 13076 and 92-008S4 were subjected to more replicate platings than 15159, due to the variations in the results. These observations support the notion that egg white is a poor culture medium for bacterial growth (Stephenson et al 1991;Baron et al 1997;Hammack et al 1993;Braun and Fehlhaber 1995).…”

Section: Resultssupporting

confidence: 81%

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence

Reed

Gehring

et al. 2008

Food Anal. Methods

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The types of chemical linkage used to bind antibodies to magnetic beads to form immunomagnetic beads (IMB) were compared in the capture and detection of Salmonella Enteriditis from egg white, egg yolk, and whole egg. Egg components were inoculated with outbreak strains of S. Enteriditis. After incubation under different conditions, IMBs derived from linking antibodies to core magnetic beads via biotin-streptavidin interactions, Schiffbase bonds and unspecified proprietary chemistry were used to capture S. Enteriditis. Europium-labeled antiSalmonella antibodies completed the sandwich, and timeresolved fluorescence served as the means of detection. For the Salmonella isolated in stationary phase and cultured from universal pre-enrichment broth (UPB), the detection signal intensity was affected by the chemistry utilized to link the antibodies to IMB, with results varying among the three test strains. When S. Enteriditis was cultured in egg yolk alone, plating data were similar to those of the growth of S. Enteriditis in UPB. Egg white by itself did not support the growth of S. Enteriditis. The addition of UPB to egg white restored the growth of Salmonella and yielded stronger detection signals than from cultures obtained from UPB with egg yolk. The detection signals obtained from the immunoassay were less intense for cultures grown in egg yolk + UPB than from cultures grown in UPB alone. The lower detection signals elicited by all IMBs suggest the availability of the antigenic groups recognized by the antibodies on IMBs was reduced in the presence of egg yolk.

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Section: Resultssupporting

confidence: 81%

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence

Reed

Gehring

et al. 2008

Food Anal. Methods

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“…the survival within and the motility from the albumen to the yolk, as well as adherence to and penetration through the vitelline membrane), we have used two different models. Penetration of Salmonella Enteritidis through the vitelline membrane has been reported in several previous studies (Braun & Fehlhaber, 1995;Gast et al, 2005). Cogan et al (2004) demonstrated that curli fimbriae are needed to attach to the vitelline membrane, in order to gain access to the yolk.…”

Section: Discussionmentioning

confidence: 94%

A comparative study on the pathogenesis of egg contamination by different serotypes ofSalmonella

et al. 2008

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Salmonella enterica serotype Enteritidis is the predominant serotype associated with egg-borne salmonellosis in humans. Apparently this serotype possesses particular characteristics that increase its chance to contaminate eggs. To identify these characteristics, two Salmonella serotype Enteritidis strains as well as one strain of each of the serotypes Salmonella Typhimurium, Salmonella Heidelberg, Salmonella Virchow and Salmonella Hadar strain were used to examine different aspects related to egg contamination. After an intravenous infection of laying hens, it was observed that the ability of the serotype Enteritidis strains to colonize the reproductive organs was significantly higher compared with the Salmonella Heidelberg, Salmonella Virchow and Salmonella Hadar strains but not with the Salmonella Typhimurium strain. Inoculating low numbers of the different Salmonella serotypes in egg albumen at 42 degrees C demonstrated that the growth of the strains belonging to the Salmonella serotypes Virchow and Hadar was seriously repressed. The other serotypes, however, survived in albumen for 24 h. Furthermore, using two different specifically designed egg infection models, it was shown that all strains used in this study were able to penetrate into and multiply inside the yolk at 25 degrees C. These findings indicate that the ability to grow in eggs post lay is not specific for the serotype Enteritidis. In conclusion, comparing strains belonging to different Salmonella serotypes has revealed that most probably a preferential colonization of the reproductive organs and an enhanced survival at 42 degrees C allows the serotype Enteritidis to contaminate eggs.

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“…This has been a serious food safety issue [47,48], although quite different from the topic addressed in this paper. Less than 10% of the shell eggs are washed and packed, and less than 0.3% of the shell eggs undergo processing for the purpose of use as food supply supplements and ingredients (i.e., dry food products, liquid products) [49]. On the other hand, China's duck egg industry is well developed and about 45% of duck eggs are processed using their traditional methods (i.e., salty eggs, century eggs) [49].…”

Section: Preventive Methods For Minimizing Contaminationmentioning

confidence: 99%

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

Seockmo¹,

Eduardo²,

Thomas³

et al. 2016

J Nutr Food Sci

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Contaminated shell egg is one of the key Salmonella infection routes for human, causing food-borne illnesses. Multiple salmonellosis infections due to egg contamination still occur in developed countries even though preventive methods, such as vaccination or washing followed by rinsing process, are carried out following certified national standards. Recent outbreaks indicated that current strategies for Salmonella control need to be optimized to further minimize contamination of commercial eggs. Therefore, there is a critical need to develop more sensitive and rapid Salmonella detection methods. In this review, we address (i) egg production; (ii) preventive methods that minimize contamination; (iii) mechanisms of Salmonella contamination; (iv) Salmonella detection methods.

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Migration of Salmonella enteritidis from the albumen into the egg yolk

Cited by 54 publications

References 5 publications

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence

A comparative study on the pathogenesis of egg contamination by different serotypes ofSalmonella

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

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