Migratory Neural Crest Cells Phagocytose Cellular Debris in the Developing Nervous System

Zhu, Yunlu; Crowley, Samantha C.; Latimer, Andrew J.; Lewis, Gwendolyn M.; Nash, Rebecca; Kucenas, Sarah

doi:10.2139/ssrn.3380256

Cited by 3 publications

(3 citation statements)

References 68 publications

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“…Thus, some other cells in the spinal cord may help to engulf the dead cells. For example, a recent zebrafish study reported that neural crest cells can help to remove apoptotic cells in the nervous system during development (Zhu et al, 2019). In the future, we will investigate whether red fluorescent protein-labeled neural crest cells could engulf dead motor neurons in our sensor zebrafish.…”

Section: Discussionmentioning

confidence: 99%

Zebrafish live imaging reveals only around 2% rather than 50% of motor neurons die through apoptosis during early development

Jia,

Yang,

Luo

2024

Preprint

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It is widely accepted that neurons will die through apoptosis if they cannot receive enough growth factors during development of vertebrates; however, there is still no real-time observation showing this dying process in live animals. Here, we generated sensor zebrafish achieving live imaging of motor neuron apoptosis at single-cell resolution. Using these sensor zebrafish, we observed for the first time that in an apoptotic motor neuron, caspase-3 activation occurred quickly within 5-6 min and at the same time between the cell body and axon. Interestingly, we found that only around 2% of motor neurons died during zebrafish development, which is much lower than the generally believed 50% cell death occurred in embryonic stage of vertebrates. Our data also showed that most of the apoptotic bodies of these dead motor neurons were not cleared by macrophages. These sensor zebrafish can serve as powerful tools to study motor neuron apoptosisin vivo.

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Section: Discussionmentioning

confidence: 99%

Zebrafish live imaging reveals only around 2% rather than 50% of motor neurons die through apoptosis during early development

Jia,

Yang,

Luo

2024

Preprint

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“…In addition, we combined the vglut2a:EGFP reporter, which labels the same V3 cell population as the vglut2a:dsRed reporter (Fig. S2), with a nkx2.2a:NLS-mCherry transgenic line (Zhu et al, 2019) to visualise the V3 cells alongside LFP progenitors. As mCherry is a stable protein, it perdures within the cell after the promotor has been switched off.…”

Section: Differentiation Dynamics Of V3 Interneurons In the Lfp Domainmentioning

confidence: 99%

“…The hsp:dnMAML-GFP (Zhao et al, 2014) and nkx2.2a:NLS-mCherry (Zhu et al, 2019) transgenic lines were generated by standard Tol2-mediated transgenesis. To generate the ptc2:Gal4 transgenic line, BAC clone zC226H23 from the CHORI-211 library that contains the ptc2 genomic region was selected for bacteria-mediated homologous recombination following standard protocols (Bussmann and Schulte-Merker, 2011).…”

Section: Generation Of Transgenic Linesmentioning

confidence: 99%

Temporal cell fate determination in the spinal cord is mediated by the duration of Notch signalling

Jacobs

Kejriwal

Kocha

et al. 2022

Developmental Biology

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Temporal cell fate determination in the spinal cord is mediated by the duration of Notch signalling

Jacobs

Kejriwal

Kocha

et al. 2021

Preprint

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During neural development, progenitor cells generate different types of neurons in specific time windows. Despite the characterisation of many of the transcription factor networks involved in these differentiation events, the mechanism behind their temporal regulation is poorly understood. To address this question, we studied the temporal differentiation of the simple lateral floor plate (LFP) domain in the zebrafish spinal cord. LFP progenitors sequentially generate early-born Kolmer-Agduhr″ (KA″) interneurons and late-born V3 interneurons. Analysis using a Notch signalling reporter demonstrates that these cell populations have distinct Notch signalling profiles. Not only do V3 cells receive higher total levels of Notch response, but they collect this response over a longer duration compared to V3 cells. To test whether the duration of Notch signalling determines the temporal cell fate specification, we combined a transgene that constitutively activates Notch signalling in the ventral spinal cord with a heat shock inducible Notch signalling terminator to switch off Notch response at any given time. Sustained Notch signalling results in expanded LFP progenitors while KA″ and V3 interneurons fail to specify. Early termination of Notch signalling leads to exclusively KA″ cell fate, despite the high level of Notch signalling, whereas late attenuation of Notch signalling drives only V3 cell fate. This suggests that the duration of Notch signalling is instructive in cell fate specification. Interestingly, knockdown experiments reveal a role for the Notch ligand Jag2b in maintaining LFP progenitors and limiting their differentiation into KA″ and V3 cells. Our results indicate that Notch signalling is required for neural progenitor maintenance while a specific attenuation timetable defines the fate of the postmitotic progeny.

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Migratory Neural Crest Cells Phagocytose Cellular Debris in the Developing Nervous System

Cited by 3 publications

References 68 publications

Zebrafish live imaging reveals only around 2% rather than 50% of motor neurons die through apoptosis during early development

Zebrafish live imaging reveals only around 2% rather than 50% of motor neurons die through apoptosis during early development

Temporal cell fate determination in the spinal cord is mediated by the duration of Notch signalling

Temporal cell fate determination in the spinal cord is mediated by the duration of Notch signalling

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