MIIP functions as a novel ligand for ITGB3 to inhibit angiogenesis and tumorigenesis of triple-negative breast cancer

Gao, Yujing; Fang, Yu-Jie; Huang, Yongli; Ma, Rui; Chen, Xixi; Wang, Fang; Pei, Xiuying; Gao, Yuanqi; Chen, Xuehua; Liu, Xinrui; Shan, Jingxuan; Li, Pu

doi:10.1038/s41419-022-05255-0

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…Additionally, it could indirectly attack tumor cells by activating anti-tumor immune responses through its derived factors such as flagellar proteins, lipoproteins, and lipopolysaccharides (J. Chen et al 2021a , b ). As a regulator of migration and invasion, MIIP, functioning as a protein, actively engages in the remodeling of the tumor cell cytoskeleton, cellular adhesion, and modulation of signaling pathways (Gao et al 2022 ). EGFR, belonging to the receptor tyrosine kinase family, exhibits a close association between its aberrant expression and activation, and the genesis, progression, invasion, and metastasis of tumors (Voldborg et al 1997 ).…”

Section: Discussionmentioning

confidence: 99%

VNP20009-Abvec-Igκ-MIIP suppresses ovarian cancer progression by modulating Ras/MEK/ERK signaling pathway

Wang,

Tang,

Dai

et al. 2024

Appl Microbiol Biotechnol

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Ovarian cancer poses a significant threat to women’s health, with conventional treatment methods encountering numerous limitations, and the emerging engineered bacterial anti-tumor strategies offer newfound hope for ovarian cancer treatment. In this study, we constructed the VNP20009-Abvec-Igκ-MIIP (VM) engineered strain and conducted initial assessments of its in vitro growth performance and the expression capability of migration/invasion inhibitory protein (MIIP). Subsequently, ID8 ovarian cancer cells and mouse cancer models were conducted to investigate the impact of VM on ovarian cancer. Our results revealed that the VM strain demonstrated superior growth performance, successfully invaded ID8 ovarian cancer cells, and expressed MIIP, consequently suppressing cell proliferation and migration. Moreover, VM specifically targeted tumor sites and expressed MIIP which further reduced the tumor volume of ovarian cancer mice (p < 0.01), via the downregulation of epidermal growth factor receptor (EGFR), Ras, p-MEK, and p-ERK. The downregulation of the PI3K/AKT signaling pathway and the decrease in Bcl-2/Bax levels also indicated VM’s apoptotic potency on ovarian cancer cells. In summary, our research demonstrated that VM exhibits promising anti-tumor effects both in vitro and in vivo, underscoring its potential for clinical treatment of ovarian cancer. Key points • This study has constructed an engineered strain of Salmonella typhimurium capable of expressing anticancer proteins • The engineered bacteria can target and colonize tumor sites in vivo • VM can inhibit the proliferation, migration, and invasion of ovarian cancer cells Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

VNP20009-Abvec-Igκ-MIIP suppresses ovarian cancer progression by modulating Ras/MEK/ERK signaling pathway

Wang,

Tang,

Dai

et al. 2024

Appl Microbiol Biotechnol

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“…With the improvement of modern whole genome sequencing, the gut microbiome in the body can be efficiently detected [ 15 ]. There also has research shown that the occurrence of tumors is directly related to the change in the intestinal microbiome, which causes the metabolites disordered in the body, and even stimulates tumor-related signaling pathways, directly inducing the formation of tumor cells [ 16 , 17 ]. So, biomarkers that alter gut microbiomes can be used as a standard to predict tumorigenesis or as an auxiliary means to diagnose tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Construction of model animals to explore intestinal microbiome for detection of breast cancer

et al. 2023

PLoS ONE

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Breast cancer ranks first among female cancers and has become a major public health problem in the current society. More studies indicated that these cancers are related to the change in the gut microbiome that can cause metabolic and immune system disorders in the body. However, there are few studies on the changes in gut microbiome caused by the onset of breast cancer, and the relationship between breast cancer and gut microbiome needs to be further clarified. In this study, we inoculated 4T1 breast cancer cells to induce breast cancer tumorigenesis in mice and collected their feces samples at different stages during this process. These intestinal florae were analyzed using 16S rRNA gene amplicon sequencing, and the results showed that at the phylum level, the ratio of Firmicutes/Bacteroidetes decreased with the development of the tumor; at the family level, the intestinal microbiome had obvious variations of Lachnospiraceae, Bacteroidaceae, Erysipelotrichaceae, etc. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and COG annotation demonstrated that decreased abundance of cancer-related signaling pathways. This study elucidated the relationship between breast cancer and intestinal microbiome, and the research results can be used as an important biomarker for the diagnosis of breast cancer.

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“…With the improvement of modern whole genome sequencing, the gut microbiome in the body can be efficiently detected [15]. There also has research shown that the occurrence of tumors is directly related to the change in intestinal microbiome, which causes the metabolites disordered in the body, and even stimulates tumor-related signaling pathways, directly inducing the formation of tumor cells [16,17]. So, biomarkers that alter gut microbiome can be used as a standard to predict tumorigenesis or as an auxiliary means to diagnose tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Construction of Model Animals to Explore Intestinal Microbiome for Detection of Breast Cancer

Xia

et al. 2023

Preprint

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Breast cancer ranks first among female cancers and has become a major public health problem in the current society. More studies indicated that these cancers are related to the change in the gut microbiome that can cause metabolic and immune system disorders in the body. However, there are few studies on the changes in gut microbiome caused by the onset of breast cancer, and the relationship between breast cancer and gut microbiome needs to be further clarified. In this study, we inoculated 4T1 breast cancer cells to induce breast cancer tumorigenesis in mice and collected their feces samples at different stages during this process. These intestinal florae were analyzed using 16S rRNA gene amplicon sequencing, and the results showed that at the phylum level, the ratio of Firmicutes/Bacteroidetes decreased with the development of the tumor; at the family level, the intestinal microbiome had obvious variations of Lachnospiraceae, Bacteroidaceae, Erysipelotrichaceae, etc. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and COG annotation demonstrated that decreased abundance of cancer related signaling pathways. This study elucidated the relationship between breast cancer and intestinal microbiome, and the research results can be used as an important biomarker for the diagnosis of breast cancer.

show abstract

MIIP functions as a novel ligand for ITGB3 to inhibit angiogenesis and tumorigenesis of triple-negative breast cancer

Cited by 7 publications

References 43 publications

VNP20009-Abvec-Igκ-MIIP suppresses ovarian cancer progression by modulating Ras/MEK/ERK signaling pathway

VNP20009-Abvec-Igκ-MIIP suppresses ovarian cancer progression by modulating Ras/MEK/ERK signaling pathway

Construction of model animals to explore intestinal microbiome for detection of breast cancer

Construction of Model Animals to Explore Intestinal Microbiome for Detection of Breast Cancer

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