Mikrobiyolojide Kullanılan Bazı Moleküler Teknikler

Cetinkaya, Esin; Ayhan, Kamuran

doi:10.7212/zkufbd.v2i1.79

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…A colony of the microorganism to be analyzed is spread on the target point and covered with the matrix. The formed mass spectra are analyzed by the appropriate software and compared with the registered profiles (12).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Karagöz¹,

Acar²,

Körkoca³

2015

Turk J Med Sci

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Background/aim: The purpose of the study was to evaluate the performance of the VITEK mass spectrometry (MS) (bioMérieux, France) system for the identification of Klebsiella spp. isolated from different sources. Moreover, while assessing the ability of the VITEK 2 automated expert system (AES) to recognize antimicrobial resistance patterns, the researchers have extended the study to compare VITEK 2 with the routine antimicrobial susceptibility testing method. Materials and methods:This study tested 51 Klebsiella spp. isolates that were isolated from environmental examples and clinical examples. Results of conventional methods and the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS were compared. Then, any differing results were compared against a reference 16S rRNA gene sequence, and when indicated, a recA sequencing analysis was done.Results: VITEK MS correctly identified 100% of the Klebsiella spp. isolates. There were two K. oxytoca isolates incorrectly identified to the species level with conventional methods according to the 16S rRNA gene sequencing analysis. In addition, a VITEK 2 AST-N261 card was used for the detection of extended spectrum beta-lactamases (ESBL). Using the VITEK 2 AES, ESBL positivity was found at the rate of 16.3% whereas this rate was 4.08% using the disk diffusion method. Conclusion:MALDI-TOF MS is a rapid and accurate method for the identification of Klebsiella spp. Moreover, the bioMérieux AES provides a useful laboratory tool for the interpretation of susceptibility results.

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Section: Introductionmentioning

confidence: 99%

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Karagöz¹,

Acar²,

Körkoca³

2015

Turk J Med Sci

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“…Biyokimyasal analizler üzerine zamandan kazanç sağlamak amacıyla günümüzde yeni yöntemler geliştirilmiştir. Bunların başında çalışılan numunenin protein spektrumunun cihazın veritabanında bulunan referans spektrumlar ile karşılaştırılması esasına dayanan VITEK Kütle spektroskopisi gelmektedir (20). Bu yöntem standart immünolojik ve biyokimyasal yöntemlerden daha güvenilir ve ucuzdur (20).…”

Section: Discussionunclassified

“…Bunların başında çalışılan numunenin protein spektrumunun cihazın veritabanında bulunan referans spektrumlar ile karşılaştırılması esasına dayanan VITEK Kütle spektroskopisi gelmektedir (20). Bu yöntem standart immünolojik ve biyokimyasal yöntemlerden daha güvenilir ve ucuzdur (20). Morfolojik ve biyokimyasal analizlere ek olarak tür düzeyinde tespit yapabilmek için çeşitli moleküler yöntemler (kromozomal DNA bileşimi, DNA-DNA sıvı hibridizasyon, 16S rRNA dizi analizi, gyrA ve/veya gyrB dizi analizleri vb.)…”

Section: Discussionunclassified

Morphological, biochemical and molecular characterization of Bacillus isolates as a producer of industrial enzymes

Karakuş¹,

Sertel²,

Duman³

et al. 2018

Turk Hij Den Biyol Derg

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“…Some phenotypic and genotypic tests are used to identify LAB. Phenotypic tests determine the characteristics of bacteria such as growth at different temperatures, resistance to high acidity and salt concentrations, gas formation from glucose, cell morphology, and thus identification from family to genus can be provided [14,15]. API 50 CHL can identify the species and subspecies by examining the carbohydrate fermentation characteristics of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Identification of Lactic Acid Bacteria in Fermented Cornichon Pickles Produced with Acid Whey and Vinegar

AVUNCA

ÇETİN

Mehmetoğlu

et al. 2022

Sakarya University Journal of Science

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The purpose of this study is to isolate, identify and compare lactic acid bacteria responsible for fermentation from pickles produced with vinegar and acid whey (AW). For this reason, fermented cornichon pickles were produced by using AW and vinegar as brine media. Colonies with different morphologies were selected and isolated at the 0, 7, 14, 21, 35, 49, 77 and 105th days of fermentation. A total of 139 isolates were obtained. Salt, acidity and pH values of brine and cornichon were followed on each analysis day. Growth and gas formation from glucose of isolates at different temperatures (10, 45, and 50 o C), salt concentrations (2, 4.5, and 10% (w/w)), different pH values (3, 4.5, and 9.6), were analyzed. Eight isolates were determined as heterofermentative according to their ability to produce CO2 from glucose. The isolates were observed to show the highest growth at 10 o C, and a very few isolates developed at 45 o C and 50 o C. No isolates were able to grow at pH 9.6, 25 of the isolates were able to grow at pH 4, and only 4 isolates were able to grow at pH 3. Only one isolate was able to show resistance to 10% salt concentration. Almost all isolates grew at 2% and 6.5% salt concentrations. 48 selected isolates were identified with API 50 CHL. 19 isolates were determined as Lactobacillus pentosus (8 vinegar, 11 AW) and 29 isolates as Lactobacillus plantarum (16 vinegar, 13 AW). As a result, L. plantarum and L. pentosus species were the dominant bacteria for cornichon pickles produced with AW and vinegar. Microbial flora in the pickles produced with two different brines were similar. The results show that AW can be successfully used for pickle production.

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Mikrobiyolojide Kullanılan Bazı Moleküler Teknikler

Cited by 5 publications

References 30 publications

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Morphological, biochemical and molecular characterization of Bacillus isolates as a producer of industrial enzymes

Identification of Lactic Acid Bacteria in Fermented Cornichon Pickles Produced with Acid Whey and Vinegar

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