Mild and Efficient Conjugation of Rabbit Fab' and Horseradish Peroxidase Using a Maleimide Compound and Its Use for Enzyme Immunoassay

Yoshitake, Shinji; Imagawa, Masayoshi; Ishikawa, Eiji; Niitsu, Yoshiro; Urushizaki, I; Nishiura, Masaru; Kanazawa, Reizo; H, Kurosaki; Tachibana, Sanro; Nakazawa, Nobuaki; Ogawa, Hiroshi

doi:10.1093/oxfordjournals.jbchem.a134065

Cited by 146 publications

(61 citation statements)

References 4 publications

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“…For the direct immunoperoxidase procedure of S-100, S-100 #-specific Fab' was prepared and was conjugated with horseradish peroxidase [Hydrogen peroxide oxidoreductase E.C. 1,11,1,7] as described by Yoshitake et al (21). As the control, the labeled anti-S-100 antibody was pretreated with 8-fold excess bovine brain S-100 and then was used for the incubation of specimens.…”

Section: Immunocytochemical Proceduresmentioning

confidence: 99%

Immunocytochemical localization of S-100 protein and calmodulin in C6 glioma cells.

Sano

Kato

Seto‐Ohshima

et al. 1984

Acta Histochem. Cytochem

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Section: Immunocytochemical Proceduresmentioning

confidence: 99%

Immunocytochemical localization of S-100 protein and calmodulin in C6 glioma cells.

Sano

Kato

Seto‐Ohshima

et al. 1984

Acta Histochem. Cytochem

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“…Conjugation consisted of three reaction steps: the introduction of maleimide groups into BSA, introduction of a sulfhydryl group into the malaria peptide and conjugation of the malaria peptide to the activated BSA. [34][35][36][37][38] To ensure that the peptide-BSA conjugate solution was not contaminated with smaller or larger complexes, it was subjected to gel filtration chromatography using a G-25 Sephadex column.…”

Section: Elisa Capture Antigenmentioning

confidence: 99%

Conservation implications of avian malaria exposure for African penguins during rehabilitation

Botes¹,

Thiart²,

Parsons³

et al. 2017

S. Afr. J. Sci.

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The African penguin (Spheniscus demersus) is the only penguin species that breeds on the African continent and it is currently classified as endangered. Its conservation is assisted by the Southern African Foundation for the Conservation of Coastal Birds (SANCCOB) which is a seabird rehabilitation facility based at the Rietvlei Wetland Reserve in Tableview, Cape Town. Despite the success of SANCCOB in rehabilitating diseased, injured or oiled penguins, significant mortalities have occurred at the facility as a result of avian malaria. Avian malaria can be contracted during rehabilitation during which penguins are inadvertently exposed to additional threats. An enzyme-linked immunosorbent assay (ELISA) was used to assess the anti-Plasmodium antibody levels of penguins to avian malaria on entry into the SANCCOB facility from 2001 to 2004 and during their rehabilitation process. Using blood smear data, avian malaria prevalence and malaria-related deaths were also monitored from 2002 to 2013. Significant increases in anti-Plasmodium antibody levels after admission were found during summer months. New infection and not parasite recrudescence was concluded to be the cause of this increase. This source was confirmed by a dramatic drop in penguin mortalities upon exclusion of mosquito vectors in 2008. Mortalities did not depend on the birds’ abilities to produce an anti-Plasmodium antibody response and oiling had no influence on immunity or prevalence of avian malaria infections. This study highlights the importance of mosquito vector control to control pathogen exposure in wild bird rehabilitation centres.

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“…Maleimido groups were introduced onto PHEG conjugates with amine-containing spacers by acylation with GMBS [30,31 ] or the water-soluble sulfo derivative of SMCC [32]. The polymer-bound maleimido groups were reacted with the thiol-containing ADR derivatives to allow for the formation of conjugates through a thioether bond (Fig.…”

Section: Preparation Of Pheg-adr Conjugatesmentioning

confidence: 99%

Synthesis and biological evaluation of immunoconjugates of adriamycin and a human IgM linked by poly[N5-2-hydroxyethyl)-l-glutamine]

Hoes

Ankone

Grootoonk

et al. 1996

Journal of Controlled Release

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The synthesis and purification of radiolabelled immunoconjugates, composed of a human IgM monoclonal antibody (IgM 16.88) directed against an intracellular tumour-associated antigen, the drug carrier poly [NS-(2-hydroxyethyl)-L-glutamine] (PHEG) and the cytostatic drug adriamycin (ADR) are described. The immunoconjugates were constructed to allow selective release of ADR in the putatively acidic environment of the tumour through a novel acid-labile maleamic acid linker. The conjugate of PHEG and the acid-labile ADR derivative effectively released ADR in cytotoxic amounts at a pH of 6.0 as judged from incubation in buffer and from inhibition of the growth of HT-29 colon tumour cells in vitro. Immunoconjugates were prepared by coupling of PHEG-ADR having a hydrolytically stable amide bond with J3q-labelled antibody through tbioether bond formation involving a single thiol group at the C-terminus of the polymer chain and maleimido groups introduced onto the * Corresponding author. Abbreviations: AcOSu,N-succinimidyl acetate; ADR, adriamycin; AEC, anion-exchange chromatography; ASI, active specific immunotherapy; BOC, tert-butox ycarbonyl ; (BOC) 20, di-tert-butyldicarbonate; BOC-AB, N-tert-butox ycarbonyl-1,4-diaminobutane; BOC-OEA, N-tert-buto xycarbonyl-2,2'-oxybis(ethylamine); CDI, N,N'-carbonyldiimidazole; CHC13, chloroform; CH2CIz, dichloromethane; CTA-I, colon tumourassociated antigen; Cys, cysteine; d. doublet; dd, double doublet; dt, double triplet; DAB, 3,3'-aminobenzidine; DMF. N,N'-dimethylformamide; DMSO, dimethylsulfoxide; DPn, number-average degree of polymerization; DPw, weight-average degree of polymerization; DSS, 4,4-dimethyl-4-silapentane sodium sulphonate; DTNB, 5,5'-dithiobis ( 2-nitrobenzoic acid) ; DTP, 4,4'-dithiodipyridine; DTT, dithiothreitol; e, molar absorbance coefficient; EEDQ, N-ethoxycarbonyl-2-ethoxy-l,2-dihydroquinoline; EIA, enzyme-linked immunoassay; Et20, diethyl ether; FABMS, fast atom bombardment mass spectrometry; FCS, fetal calf serum; GABA, y-aminobutyric acid; GMB, y-maleimidobutyryl; GMBS, N-(ymaleimidobutyryloxy)succinimide; HOAc, acetic acid; HONSu, N-hydroxysuccinimide; HPMA, N-(2-hydroxypropyl)methacrylamide; HP-SEC, high-performance size exclusion chromatography; HRP, horseradish peroxidase; LALLS, low-angle laser light scattering; m, multiplet; M,. number-average molecular weight; Mw, weight-average molecular weight; MRW, mean residue weight; MoAb, monoclonal antibody: MSA, methanesulphonic acid; MTT, 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; NAN,, sodium azide; NCA, N-carboxyanhydride; PBLG, poly( T-benzyl-L-glutamate); PBS, phosphate-buffered saline; PDT, 2-pyridyldithio; PGA, poly Hoes et al. / Journal of Controlled Release 38 (1996) antibody. The immunoreactivity of IgM-PHEG-ADR conjugate was almost fully preserved. Tumour uptake and biodistribution of ~251-1abelled PHEG-ADR and of ~3q-labelled IgM-PHEG-ADR, which was co-administered with ~H-labelled IgM 16.88, in nude mice carrying MRI-W207 human ovarian tumour xenografts were s...

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Mild and Efficient Conjugation of Rabbit Fab' and Horseradish Peroxidase Using a Maleimide Compound and Its Use for Enzyme Immunoassay

Cited by 146 publications

References 4 publications

Immunocytochemical localization of S-100 protein and calmodulin in C6 glioma cells.

Immunocytochemical localization of S-100 protein and calmodulin in C6 glioma cells.

Conservation implications of avian malaria exposure for African penguins during rehabilitation

Synthesis and biological evaluation of immunoconjugates of adriamycin and a human IgM linked by poly[N5-2-hydroxyethyl)-l-glutamine]

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