Mild diabetes: long‐term effects on gastric motility evaluated in rats

Hauschildt, Andrieli Taise; Corá, Luciana Aparecida; Volpato, Gustavo Tadeu; Sinzato, Yuri Karen; Damasceno, Débora Cristina; Américo, Madileine Francely

doi:10.1111/iep.12262

Cited by 11 publications

(8 citation statements)

References 66 publications

(141 reference statements)

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“…In our study, both the F1 and F2 generations had changes in gastric motility and, in parallel, their glycemic levels were higher compared to the CONT and F3 groups. Our group has already demonstrated the relationship between these variables in mildly diabetic rats ( 25 ). The severity of gastric dysmotility and gastric repercussions seems to be inversely proportional to glycemic control ( 26 ).…”

Section: Discussionmentioning

confidence: 85%

Paternal obesity and its transgenerational effects on gastrointestinal function in male rat offspring

Machado

Gama

Beckmann

et al. 2021

Braz J Med Biol Res

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The interplay between obesity and gastrointestinal (GI) motility is contradictory, and the transgenerational influence on this parameter is unknown. We aimed to evaluate the GI function in a model of paternal obesity and two subsequent generations of their male offspring. Newborn male rats were treated with monosodium glutamate (MSG) and composed the F1 generation, while control rats (CONT) received saline. At 90 days, male F1 were mated with non-obese females to obtain male offspring (F2), which later mated with non-obese females for obtaining male offspring of F3 generation. Lee Index analysis was adopted to set up the obesity groups. Alternating current biosusceptometry (ACB) technique was employed to calculate GI transit parameters: mean gastric emptying time (MGET), mean cecum arrival time (MCAT), mean small intestinal transit time (MSITT), and gastric frequency and amplitude of contractions. Glucose, insulin, and leptin levels and duodenal morphometry were measured. F1 obese rats showed a decrease in the frequency and amplitude of gastric contractions, while obese rats from the F2 generation showed accelerated MGET and delayed MCAT and MSITT. Glucose and leptin levels were increased in F1 and F2 generations. Insulin levels decreased in F1, F2, and F3 generations. Duodenal morphometry was altered in all three generations. Obesity may have paternal transgenerational transmission, and it provoked disturbances in the gastrointestinal function of three generations.

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Section: Discussionmentioning

confidence: 85%

Paternal obesity and its transgenerational effects on gastrointestinal function in male rat offspring

Machado

Gama

Beckmann

et al. 2021

Braz J Med Biol Res

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“…Additionally, rapid gastric emptying of liquids has been associated with type 2 DM, particularly in the early stages of the disease, which may persist even in the late stage. In rats with mild diabetes, gastric emptying was faster after treatment with streptozotocin when compared with control animals (30). Acute hyperglycemia might be thought to cause rapid gastric emptying by stimulating the gastric excitatory vagal motor circuit (26).…”

Section: Discussionmentioning

confidence: 98%

Disturbed gastric motility in patients with long-standing diabetes mellitus

Kamiya

Fukuta

Hagiwara

et al. 2022

J Smooth Muscle Res

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Purpose: Gastric dysmotility has been reported in patients with long-standing diabetes mellitus (DM). Some patients with DM are diagnosed as diabetes gastroparesis and have several upper gastrointestinal (GI) symptoms such as appetite loss and abdominal pain. This study aimed to identify the relationship between gastric motility and upper GI symptoms in patients with long-standing DM. Method: This study was conducted among 23 patients with DM and 15 healthy controls. All the patients with DM were receiving insulin treatment and had at least one history of incidence of diabetic nephropathy, retinopathy or neuropathy.Gastric motility was evaluated using electrogastrography (EGG) and gastric emptying using the 13 C-acetic acid breath test. The most severe upper gastrointestinal symptoms were assessed in all patients. Results: Compared to healthy controls, patients with long-standing DM showed a significantly lower percentage of normogastria at the postprandial state with a lower power ratio in EGG. Gastric emptying was significantly delayed in patients with DM in the overall analysis. Sixteen patients with DM (69.6%) demonstrated abnormalities in either gastric myoelectrical activity or gastric emptying. Among patients with abnormal EGG or delayed gastric emptying, 12 had some GI symptoms, compared with 3 patients with normal gastric motility.No significant correlation was observed between the gastric emptying parameters and HbA1c values. Conclusion: Patients with long-standing DM showed gastric dysmotility, including impaired gastric myoelectrical activity and delayed gastric emptying. Gastric dysmotility appears to be closely correlated with upper GI symptoms in patients with long-standing DM.

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“…The ACB technique was applied for the first time in mice. It was previously used for gastrointestinal motor studies in humans, dogs, and rats …”

Section: Discussionmentioning

confidence: 99%

Gastrointestinal motility and morphology in mice: Strain‐dependent differences

Gama

Machado

Beckmann

et al. 2020

Neurogastroenterology Motil

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Background: BALB/c and C57BL/6 mice are widely used in biomedical research; however, the differences between strains are still underestimated. Our aims were to develop an experimental protocol to evaluate the duodenal contractility and gastrointestinal transit in mice using the Alternating Current Biosusceptometry (ACB) technique and to compare gastrointestinal motor function and morphology between BALB/c and C57BL/6 strains. Methods: Male mice were used in experiments (a) duodenal contractility: animals which had a magnetic marker surgically fixed in the duodenum to determine the frequency and amplitude of contractions and (b) gastrointestinal transit: animals which ingested a magnetically marked chow to calculate the Oro-Anal Transit Time (OATT) and the Fecal Pellet Elimination Rate (FPER). The animals were killed after the experiments for organ collection and morphometric analysis.Key Results: BALB/c and C57BL/6 had two different duodenal frequencies (high and low) with similar amplitudes. After 10 hours of monitoring, BALB/c eliminated around 89% of the ingested marker and C57BL/6 eliminated 33%; OATT and FPER were slower for C57BL/6 compared with BALB/c. The OATT and amplitude of low frequency had a strong positive correlation in C57BL/6. For BALB/c, the gastric muscular layer was thicker compared to that measured for C57BL/6. Conclusions and Inferences:The experimental protocol to evaluate duodenal contractility and fecal magnetic pellets output using the ACB technique in mice was successfully established. BALB/c strains had higher duodenal frequencies and a shorter time to eliminate the ingested marker. Our results showed differences in both motor function and gastrointestinal morphology between BALB/c and C57BL/6 strains. K E Y W O R D S BALB/c, C57BL/6, duodenal contractility, gastrointestinal transit, inbred S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section.

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Mild diabetes: long‐term effects on gastric motility evaluated in rats

Cited by 11 publications

References 66 publications

Paternal obesity and its transgenerational effects on gastrointestinal function in male rat offspring

Paternal obesity and its transgenerational effects on gastrointestinal function in male rat offspring

Disturbed gastric motility in patients with long-standing diabetes mellitus

Gastrointestinal motility and morphology in mice: Strain‐dependent differences

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