Mild hypothermia inhibits the Notch 3 and Notch 4 activation and seizure after stroke in the rat model

Yang, Guoshuai; Zhou, Xiaoyan; An, Xue-Fang; Liu, Xuan-Jun; Zhang, Yanjun; Yu, Dan

doi:10.1016/j.prp.2018.05.003

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…It was previously reported that MH reduces brain hemorrhage and blood-brain barrier disruption after stroke (7), and that it may alleviate cerebral ischemic injury in diabetic patients through promoting autophagy and inhibiting pyroptosis (8). Researchers also demonstrated that inhibition of Notch3 and Notch4 signaling is involved in the protective effect of MH against cerebral ischemic injury (9). Moreover, MH promotes long-term white matter integrity and inhibits neuroinflammation in mice with ischemic brain injury (10).…”

Section: Introductionmentioning

confidence: 97%

Protective effects of combined treatment with mild hypothermia and edaravone against cerebral ischemia/reperfusion injury via oxidative stress and Nrf2 pathway regulation

Wang

et al. 2020

Int J Oncol

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Mild hypothermia (MH) and edaravone (EDA) exert neuroprotective effects against cerebral ischemia/reperfusion (I/R) injury through activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. However, whether MH and EDA exert synergistic effects against cerebral I/R injury remains unknown. The aim of the present study was to investigate the effects and mechanism of action of MH in combination with EDA in cerebral I/R injury. A rat cerebral I/R injury model was constructed by middle cerebral artery occlusion (MCAO) followed by reperfusion, and the mice were treated by MH, EDA or the inhibitor of the Nrf2 signaling pathway brusatol (Bru). It was observed that mice treated by MCAO had higher neurological deficit scores and oxidative stress levels, and low spatial learning and memory capacity; moreover, the CA1 region of the hippocampi of the mice exhibited reduced neuronal density and viability, and reduced mitochondrial dysfunction. However, MH in combination with EDA reversed the effects of MCAO, which were blocked by Bru injection. The levels of glutathione (GSH), GSH peroxidase, catalase and superoxide dismutase in rat ischemic hemisphere tissues were reduced by Bru. Western blotting demonstrated that the combined treatment with MH and EDA promoted the nuclear localization of Nrf2, and increased the levels of NAD(P)H quinone oxidoreductase and heme oxygenase (HO)-1. In conclusion, MH combined with EDA exerted synergistic neuroprotective effects against cerebral I/R injury involving changes in the Nrf2/HO-1 pathway.

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Section: Introductionmentioning

confidence: 97%

Protective effects of combined treatment with mild hypothermia and edaravone against cerebral ischemia/reperfusion injury via oxidative stress and Nrf2 pathway regulation

Wang

et al. 2020

Int J Oncol

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“… 12 Previous studies have shown that the Notch1 and Notch3 proteins stimulate tumors and cell proliferation and neural differentiation in the central nervous system. 13 , 14 , 15 , 16 However, whether Notch3 and its downstream target genes are involved in the effects of propofol on the proliferation and differentiation of hippocampal NSCs remains unclear. This study was designed to explore the concentration-dependent effects of propofol on learning and memory in adult male rats, as well as its effects on the proliferation and differentiation of hippocampal NSCs.…”

Section: Introductionmentioning

confidence: 99%

Effects of Subchronic Propofol Administration on the Proliferation and Differentiation of Neural Stem Cells in Rat Hippocampus

Chang

Bai

et al. 2023

Current Therapeutic Research

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“…The effectiveness of achieving mild hypothermia has been evaluated in several experimental models of brain damage in ischaemic stroke 21,22 . Clinical reports indicate that mild hypothermia can reduce brain oedema due to stroke 23 .…”

Section: Introductionmentioning

confidence: 99%

“…19 The effectiveness of achieving mild hypothermia has been evaluated in several experimental models of brain damage in ischaemic stroke. 21,22 Clinical reports indicate that mild hypothermia can reduce brain oedema due to stroke. 23 Mild hypothermia was induced via surface cooling methods and acetaminophen therapy in clinical studies and has proven safety and feasibility in stroke patients.…”

mentioning

confidence: 99%

Role of PERK‐mediated pathway in the effect of mild hypothermia after cerebral ischaemia/reperfusion

Zhao

Xia

Tang

et al. 2023

Eur J Clin Investigation

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BackgroundHypothermia is an effective method of reducing brain injury caused by a variety of neurological insults. It is aimed to elucidate whether a change in the expression of PERK‐mediated pathway proteins is an indicator of the neuroprotective effect of mild hypothermia after cerebral ischaemia/reperfusion.MethodsOne hundred and ninety‐two male C57BL/6 mice were randomly divided into three groups: a sham group, a cerebral normothermic ischaemia/reperfusion (I/R) group and a cerebral hypothermic I/R group. A cerebral ischaemia model was established by ligating the bilateral common carotid artery for 15 min. Mice in the hypothermia group stayed in a cage that was set at 33°C, sprayed with a spray of 70% ethanol, and blown with two high‐speed fans. The state of neurons was assessed on micropreparations stained with haematoxylin–eosin and TUNEL. The expressions of GRP78, p‐perk, p‐eif2α, ATF4 and CHOP were measured by western blot analysis 6, 12, 24 and 72 h after reperfusion.ResultsThe number of surviving cells was significantly higher in the hypothermia group than in the group without hypothermia (p < .05). The GRP78 expression in the hypothermia group was statistically higher (p < .05) than in the ischaemia/reperfusion group. Optical densities of p‐perk, p‐eif2α and ATF4 in hippocampus CA1 neurons ischaemia were statistically significantly lower in the hypothermia group than in the ischaemia/reperfusion group (p < .05). The CHOP expression in the hypothermia group was statistically lower (p < .05) than in the ischaemia/reperfusion group.ConclusionMild hypothermia for 6 h promoted moderate neuroprotection by mediating the expression of GRP78, p‐PERK, p‐eIF2α, ATF4 and CHOP.

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Mild hypothermia inhibits the Notch 3 and Notch 4 activation and seizure after stroke in the rat model

Cited by 7 publications

References 33 publications

Protective effects of combined treatment with mild hypothermia and edaravone against cerebral ischemia/reperfusion injury via oxidative stress and Nrf2 pathway regulation

Protective effects of combined treatment with mild hypothermia and edaravone against cerebral ischemia/reperfusion injury via oxidative stress and Nrf2 pathway regulation

Effects of Subchronic Propofol Administration on the Proliferation and Differentiation of Neural Stem Cells in Rat Hippocampus

Role of PERK‐mediated pathway in the effect of mild hypothermia after cerebral ischaemia/reperfusion

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