Mild iron overload induces TRIP12-mediated degradation of YY1 to trigger hepatic inflammation

Tang, Yuxiao; Wang, Dongyao; Niu, Xiaowen; Wu, Hui‐Wen; Yang, Jianmin; Zhang, Yinyin; Song, Shangjin; Lv, Diya; Chai, Yifeng; Lu, Hongtao; Shen, Hui; Chen, Ling; Li, Min

doi:10.1016/j.freeradbiomed.2020.10.013

Cited by 21 publications

(15 citation statements)

References 48 publications

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“…Western Blot assays were performed and analyzed as before ( Tang et al, 2020b ). Briefly, a whole extract was prepared by a Total Protein Extraction Kit (Keygene Biotech, Nanjing, China).…”

Section: Methodsmentioning

confidence: 99%

An iron-deficient diet prevents alcohol- or diethylnitrosamine-induced acute hepatotoxicity in mice by inhibiting ferroptosis

Gao

Wang

Zhang

et al. 2022

Current Research in Food Science

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“…Western Blot assays were performed and analyzed as before ( Tang et al, 2020b ). Briefly, a whole extract was prepared by a Total Protein Extraction Kit (Keygene Biotech, Nanjing, China).…”

Section: Methodsmentioning

confidence: 99%

An iron-deficient diet prevents alcohol- or diethylnitrosamine-induced acute hepatotoxicity in mice by inhibiting ferroptosis

Gao

Wang

Zhang

et al. 2022

Current Research in Food Science

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“…Previous studies have demonstrated that E3 ubiquitin ligase Smurf2 regulates proteasomal degradation of YY1 in multiple model systems [40][41][42][43]. In addition, E3 ubiquitin ligase TRIP12 interacts with and ubiquitinates YY1, leading to its degradation [50]. In contrast, ubiquitin specific protease 21 (USP21) stabilizes YY1 in non-small-cell lung cancer cells via mediating its deubiquitination [51].…”

Section: Discussionmentioning

confidence: 99%

RNF144A exerts tumor suppressor function in breast cancer through targeting YY1 for proteasomal degradation to downregulate GMFG expression

et al. 2022

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Ring finger protein 144A (RNF144A), a poorly characterized member of the RING-in-between-RING family of E3 ubiquitin ligases, is an emerging tumor suppressor, but its underlying mechanism remains largely elusive. To address this issue, we used Affymetrix GeneChip Human Transcriptome Array 2.0 to profile gene expression in MDA-MB-231 cells stably expressing empty vector pCDH and Flag-RNF144A, and found that 128 genes were differentially expressed between pCDH- and RNF144A-expressing cells with fold change over 1.5. We further demonstrated that RNF144A negatively regulated the protein and mRNA levels of glial maturation factor γ (GMFG). Mechanistical investigations revealed that transcription factor YY1 transcriptionally activated GMFG expression, and RNF144A interacted with YY1 and promoted its ubiquitination-dependent degradation, thus blocking YY1-induced GMFG expression. Functional rescue assays showed that ectopic expression of RNF144A suppressed the proliferative, migratory, and invasive potential of breast cancer cells, and the noted effects were partially restored by re-expression of GMFG in RNF144A-overexpressing breast cancer cells. Collectively, these findings reveal that RNF144A negatively regulates GMFG expression by targeting YY1 for proteasomal degradation, thus inhibiting the proliferation, migration, and invasion of breast cancer cells.

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“…All sections were later counterstained with DAPI to label nuclei at room temperature for 8 min. Finally, an anti-fluorescence quenching agent ( 24 ) was used to seal the slides. Digital images were captured with a Leica DMI4000B microscope (Leica, Germany).…”

Section: Methodsmentioning

confidence: 99%

Combined effects of EMP and RF field on emotional behavior in mice

Qin

Liu

Wang

et al. 2023

Front. Public Health

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BackgroundRecently, concerns about the combined effects of electromagnetic field (EMF) in daily living and occupational environment are rapidly growing.MethodsIn this study, we investigated the combined effects of 1-week exposure to electromagnetic pulse (EMP) at 650 kV/m for 1,000 pulses and 4.9 GHz radiofrequency (RF) at 50 W/m2 for 1 h/d in male mice. Open field test, tail suspension test and Y-maze were applied to evaluate anxiety, depression-like behaviors and spatial memory ability, respectively.ResultsIt was found that compared with Sham group, combined exposure to EMP and RF induced anxiety-like behavior, increased the level of serum S100B and decreased the level of serum 5-HT. The results of quantitative proteomic and KEGG analysis showed that the differentially expressed proteins in hippocampus were enriched in Glutamatergic and GABAergic synapse after combined exposure group, which were verified by western blot. In addition, an obvious histological alteration and autophagy-associated cell death were observed in amygdala instead of hippocampus after combined exposure to EMP and 4.9 GHz RF.ConclusionCombined exposure to EMP and 4.9 GHz RF could induce emotional behavior alteration, which might be associated with Glutamatergic and GABAergic synapse system of hippocampus and autophagy in amygdala.

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Mild iron overload induces TRIP12-mediated degradation of YY1 to trigger hepatic inflammation

Cited by 21 publications

References 48 publications

An iron-deficient diet prevents alcohol- or diethylnitrosamine-induced acute hepatotoxicity in mice by inhibiting ferroptosis

An iron-deficient diet prevents alcohol- or diethylnitrosamine-induced acute hepatotoxicity in mice by inhibiting ferroptosis

RNF144A exerts tumor suppressor function in breast cancer through targeting YY1 for proteasomal degradation to downregulate GMFG expression

Combined effects of EMP and RF field on emotional behavior in mice

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