Mild traumatic brain injury in Drosophila melanogaster alters reactive oxygen and nitrogen species in a sex-dependent manner

Jones, T. Bucky; Mackey, Tracy; Juba, Amber N.; Amin, Kush; Atyam, Amruth; McDole, Madison; Yancy, Jarod; Thomas, Theresa Currier; Buhlman, Lori M.

doi:10.1016/j.expneurol.2023.114621

Cited by 6 publications

(2 citation statements)

References 79 publications

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“…Interestingly, injury-induced suppression of Metchnikowin and Diptericin and increased expression of PPO2 observed at the chronic timepoint is correlated with female-specific late-life behavioral deficits and brain pathology. This finding is supported by a recent report that mated female flies exhibited decreased markers of oxidative stress following trauma, which could be due to immune suppression following a TBIinduced immune challenge 92 . Additionally, work comparing bacterial infection in virgin, SP 0mated, and wildtype-mated females suggest that mating and particularly egg-laying reduces the female's overall ability to defend against bacterial infections, leading to decreased expression of AMP genes after infections 68 .…”

Section: Discussionsupporting

confidence: 83%

Sexual Dimorphism in Age-Dependent Neurodegeneration After Mild Head Trauma inDrosophila: Unveiling the Adverse Impact of Female Reproductive Signaling

Ye,

Ho,

Moberg

et al. 2024

Preprint

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Environmental insults, including mild head trauma, significantly increase the risk of neurodegeneration. However, it remains challenging to establish a causative connection between early-life exposure to mild head trauma and late-life emergence of neurodegenerative deficits, nor do we know how sex and age compound the outcome. Using a Drosophila model, we demonstrate that exposure to mild head trauma causes neurodegenerative conditions that emerge late in life and disproportionately affect females. Age-at-injury further exacerbates this effect in a sexually dimorphic manner. We further identify Sex Peptide (SP) signaling as a key factor in female susceptibility to post-injury brain deficits. RNA sequencing highlights changes in innate immune defense transcripts specifically in mated females during late life. Our findings establish a causal relationship between early head trauma and late-life neurodegeneration, emphasizing sex differences in injury response and the impact of age-at-injury. Finally, our findings reveal that reproductive signaling adversely impacts female response to mild head insults and elevates vulnerability to late-life neurodegeneration.

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Section: Discussionsupporting

confidence: 83%

Sexual Dimorphism in Age-Dependent Neurodegeneration After Mild Head Trauma inDrosophila: Unveiling the Adverse Impact of Female Reproductive Signaling

Ye,

Ho,

Moberg

et al. 2024

Preprint

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“…The development of robust chronic neurotransmitter sensing systems that can investigate the brain in the days and weeks following implantation is of great interest. For investigations correlating neurotransmitters to animal behavior, a postsurgical recovery period is required to ensure there are no confounding effects of the surgery or anesthesia on behavior or neurotransmission. , Investigating the progression of disease, treatment, and recovery over time is also of interest. ,,− Generally, separate populations of animals are required for each time point, but advances in sensor stability may give researchers access to high-quality longitudinal neurotransmitter data within a single population of animals. While there are certainly many reports of enzyme biosensors with nanomaterial coatings that have improved in vitro stability, in vivo stability is only rarely investigated. , …”

Section: Resultsmentioning

confidence: 99%

Improving Sensitivity and Longevity of In Vivo Glutamate Sensors with Electrodeposited NanoPt

Robbins,

Wong,

Pwint

et al. 2024

ACS Appl. Mater. Interfaces

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In vivo glutamate sensing has provided valuable insight into the physiology and pathology of the brain. Electrochemical glutamate biosensors, constructed by cross-linking glutamate oxidase onto an electrode and oxidizing H 2 O 2 as a proxy for glutamate, are the gold standard for in vivo glutamate measurements for many applications. While glutamate sensors have been employed ubiquitously for acute measurements, there are almost no reports of long-term, chronic glutamate sensing in vivo, despite demonstrations of glutamate sensors lasting for weeks in vitro. To address this, we utilized a platinum electrode with nanometer-scale roughness (nanoPt) to improve the glutamate sensors' sensitivity and longevity. NanoPt improved the GLU sensitivity by 67.4% and the sensors were stable in vitro for 3 weeks. In vivo, nanoPt glutamate sensors had a measurable signal above a control electrode on the same array for 7 days. We demonstrate the utility of the nanoPt sensors by studying the effect of traumatic brain injury on glutamate in the rat striatum with a flexible electrode array and report measurements of glutamate taken during the injury itself. We also show the flexibility of the nanoPt platform to be applied to other oxidase enzyme-based biosensors by measuring γ-aminobutyric acid in the porcine spinal cord. NanoPt is a simple, effective way to build high sensitivity, robust biosensors harnessing enzymes to detect neurotransmitters in vivo.

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Research progress of metalloporphyrin against neurodegen-erative diseases

CHEN,

LU,

WANG

et al. 2024

J Zhejiang Univ (Med Sci)

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Mild traumatic brain injury in Drosophila melanogaster alters reactive oxygen and nitrogen species in a sex-dependent manner

Cited by 6 publications

References 79 publications

Sexual Dimorphism in Age-Dependent Neurodegeneration After Mild Head Trauma inDrosophila: Unveiling the Adverse Impact of Female Reproductive Signaling

Sexual Dimorphism in Age-Dependent Neurodegeneration After Mild Head Trauma inDrosophila: Unveiling the Adverse Impact of Female Reproductive Signaling

Improving Sensitivity and Longevity of In Vivo Glutamate Sensors with Electrodeposited NanoPt

Research progress of metalloporphyrin against neurodegen-erative diseases

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