Milk and sugar: Regulation of cell wall synthesis in the milk yeast Kluyveromyces lactis

Backhaus, Katja; Buchwald, Ulf; Heppeler, Nele; Schmitz, Hans‐Peter; Rodicio, Rosaura; Heinisch, Jürgen J.

doi:10.1016/j.ejcb.2011.04.005

Cited by 13 publications

(22 citation statements)

References 46 publications

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“…However, both yeasts differ in certain physiological properties that considerably affect their usability. Thus, K. lactis and S. cerevisiae display significant differences in protein glycosylation, cell wall biosynthesis and protein secretion (reviewed by Backhaus et al ., 2011 [57]), which may have important implications for the intracellular localization, folding, stability and immunogenicity of heterologously expressed proteins [58].…”

Section: Discussionmentioning

confidence: 99%

Protective Vaccination against Infectious Bursal Disease Virus with Whole Recombinant Kluyveromyces lactis Yeast Expressing the Viral VP2 Subunit

et al. 2012

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Here we report on vaccination approaches against infectious bursal disease (IBD) of poultry that were performed with complete yeast of the species Kluyveromyces lactis (K. lactis). Employing a genetic system that enables the rapid production of stably transfected recombinant K. lactis, we generated yeast strains that expressed defined quantities of the virus capsid forming protein VP2 of infectious bursal disease virus (IBDV). Both, subcutaneous as well as oral vaccination regiments with the heat-inactivated but otherwise untreated yeast induced IBDV-neutralizing antibodies in mice and chickens. A full protection against a subsequent IBDV infection was achieved by subcutaneous inoculation of only milligram amounts of yeast per chicken. Oral vaccination also generated protection: while mortality was observed in control animals after virus challenge, none of the vaccinees died and ca. one-tenth were protected as indicated by the absence of lesions in the bursa of Fabricius. Recombinant K. lactis was thus indicated as a potent tool for the induction of a protective immune response by different applications. Subcutaneously applied K. lactis that expresses the IBDV VP2 was shown to function as an efficacious anti-IBD subunit vaccine.

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Section: Discussionmentioning

confidence: 99%

Protective Vaccination against Infectious Bursal Disease Virus with Whole Recombinant Kluyveromyces lactis Yeast Expressing the Viral VP2 Subunit

et al. 2012

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“…For K. lactis , basically similar components to those described for S. cerevisiae seem to be involved in cell integrity signalling (Backhaus et al , ). However, phenotypes of mutants in specific components can differ drastically between S. cerevisiae and K. lactis , suggesting that the latter, with its more respiratory metabolism, may provide additional insights into how exactly the components of the signalling pathway interact with each other to trigger an appropiate cellular response (Backhaus et al , ; Rodicio and Heinisch, ). Former studies revealed alterations in K. lactis membrane‐lipid composition when grown in the presence of subinhibitory doses of amphotericin B and have also shown that polyenes can directly affect the enzyme S‐adenosylmethionine sterol C‐24 methyltransferase, involved in the biosynthesis of ergosterol (Coulon et al ., ; Hakkou et al ., ; Mukhtar et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…This largely explains why K. lactis displays a much lower redundancy of genes whose products are involved in central metabolism or signal transduction pathways, as compared with S. cerevisiae . For K. lactis , basically similar components to those described for S. cerevisiae seem to be involved in cell integrity signalling (Backhaus et al , ). However, phenotypes of mutants in specific components can differ drastically between S. cerevisiae and K. lactis , suggesting that the latter, with its more respiratory metabolism, may provide additional insights into how exactly the components of the signalling pathway interact with each other to trigger an appropiate cellular response (Backhaus et al , ; Rodicio and Heinisch, ).…”

Section: Introductionmentioning

confidence: 99%

ERG6gene deletion modifiesKluyveromyces lactissusceptibility to various growth inhibitors

Konecna

Hervay

Valachovič

et al. 2016

Yeast

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The ERG6 gene encodes an S-adenosylmethionine dependent sterol C-24 methyltransferase in the ergosterol biosynthetic pathway. In this work we report the results of functional analysis of the Kluyveromyces lactis ERG6 gene. We cloned the KlERG6 gene, which was able to complement the erg6Δ mutation in both K. lactis and Saccharomyces cerevisiae. The lack of ergosterol in the Klerg6 deletion mutant was accompanied by increased expression of genes encoding the last steps of the ergosterol biosynthesis pathway as well as the KlPDR5 gene encoding an ABC transporter. The Klerg6Δ mutation resulted in reduced cell susceptibility to amphotericin B, nystatin and pimaricin and increased susceptibility to azole antifungals, fluphenazine, terbinafine, brefeldin A and caffeine. The susceptibility phenotype was suppressed by the KlPDR16 gene encoding one of the phosphatidylinositol transfer proteins belonging to the Sec14 family. Decreased activity of KlPdr5p in Klerg6Δ mutant (measured as the ability to efflux rhodamine 6G) together with increased amount of KlPDR5 mRNA suggest that the zymosterol which accumulates in the Klerg6Δ mutant may not fully compensate for ergosterol in the membrane targeting of efflux pumps. These results point to the fact that defects in sterol transmethylation appear to cause a multitude of physiological effects in K. lactis cells. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Since CWI signaling functions seem to be similar in K. lactis and S. cerevisiae (8), it would be interesting to see which of the remaining sensors is involved in the oxidative-stress response of the milk yeast. In the more distantly related yeast Schizosaccharomyces pombe, Wsc1 and Mid2 homologs have also been characterized (with the latter designated SpMtl2), but they did not trigger the CWI pathway (40).…”

Section: Not All Sensors Are the Samementioning

confidence: 99%

Up against the Wall: Is Yeast Cell Wall Integrity Ensured by Mechanosensing in Plasma Membrane Microdomains?

Kock

Dufrêne

Heinisch

2015

Appl Environ Microbiol

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bYeast cell wall integrity (CWI) signaling serves as a model of the regulation of fungal cell wall synthesis and provides the basis for the development of antifungal drugs. A set of five membrane-spanning sensors (Wsc1 to Wsc3, Mid2, and Mtl1) detect cell surface stress and commence the signaling pathway upon perturbations of either the cell wall structure or the plasma membrane. We here summarize the latest advances in the structure/function relationship primarily of the Wsc1 sensor and critically review the evidence that it acts as a mechanosensor. The relevance and physiological significance of the information obtained for the function of the other CWI sensors, as well as expected future developments, are discussed.

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Milk and sugar: Regulation of cell wall synthesis in the milk yeast Kluyveromyces lactis

Cited by 13 publications

References 46 publications

Protective Vaccination against Infectious Bursal Disease Virus with Whole Recombinant Kluyveromyces lactis Yeast Expressing the Viral VP2 Subunit

Protective Vaccination against Infectious Bursal Disease Virus with Whole Recombinant Kluyveromyces lactis Yeast Expressing the Viral VP2 Subunit

ERG6gene deletion modifiesKluyveromyces lactissusceptibility to various growth inhibitors

Up against the Wall: Is Yeast Cell Wall Integrity Ensured by Mechanosensing in Plasma Membrane Microdomains?

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