“…47 These results are similar to a study that showed an increase in Firmicutes after kefir consumption by healthy mice. 44 However, those results are opposite to another study with a CRC mouse model that kefir consumption reduced the Firmicutes/Bacteroidetes ratio, Ascomycota/Basidiomycota ratio, Clostridium sensu stricto, Aspergillus , and increased probiotic bacterias. 48 This dysbiosis observed in our study can influence several features of intestinal health including its physiology, cellular features, development, metabolism, and immune homeostasis.…”
Section: Discussionmentioning
confidence: 78%
“…30,41–43 This discrepancy can be associated with the type of milk used, concentration of grains/milk, and either the traditional or industrial fermentation procedure. 44 Also, the number and species of animals per group, time of the experiment, different methodologies applied, and the previously established gut microbiota can influence the research results. 45,46 Then, in this experiment the observed results may be associated with the profile found in the intestinal microbiota of the animals.…”
The yacon flour improved the composition of the intestinal microbiota and the production of acetate and reduce the pH. Kefir was not able to improve the intestinal microbiota and increasing the development of tumors.
“…47 These results are similar to a study that showed an increase in Firmicutes after kefir consumption by healthy mice. 44 However, those results are opposite to another study with a CRC mouse model that kefir consumption reduced the Firmicutes/Bacteroidetes ratio, Ascomycota/Basidiomycota ratio, Clostridium sensu stricto, Aspergillus , and increased probiotic bacterias. 48 This dysbiosis observed in our study can influence several features of intestinal health including its physiology, cellular features, development, metabolism, and immune homeostasis.…”
Section: Discussionmentioning
confidence: 78%
“…30,41–43 This discrepancy can be associated with the type of milk used, concentration of grains/milk, and either the traditional or industrial fermentation procedure. 44 Also, the number and species of animals per group, time of the experiment, different methodologies applied, and the previously established gut microbiota can influence the research results. 45,46 Then, in this experiment the observed results may be associated with the profile found in the intestinal microbiota of the animals.…”
The yacon flour improved the composition of the intestinal microbiota and the production of acetate and reduce the pH. Kefir was not able to improve the intestinal microbiota and increasing the development of tumors.
“…Notably, Lachnoclostridium and Parasutterella exhibited a substantial upregulation after PSHC therapy. It was worth mentioning that these two upregulated genera were considered core genera involved in gut SCFA synthesis and regulation of signaling pathways (Albuquerque Pereira et al 2023 ). Fig.…”
The high recurrence rate of renal uric acid stone (UAS) poses a significant challenge for urologists, and potassium sodium hydrogen citrate (PSHC) has been proven to be an effective oral dissolution drug. However, no studies have investigated the impact of PSHC on gut microbiota and its metabolites during stone dissolution therapy. We prospectively recruited 37 UAS patients and 40 healthy subjects, of which 12 patients completed a 3-month pharmacological intervention. Fasting vein blood was extracted and mid-stream urine was retained for biochemical testing. Fecal samples were collected for 16S ribosomal RNA (rRNA) gene sequencing and short chain fatty acids (SCFAs) content determination. UAS patients exhibited comorbidities such as obesity, hypertension, gout, and dyslipidemia. The richness and diversity of the gut microbiota were significantly decreased in UAS patients, Bacteroides and Fusobacterium were dominant genera while Subdoligranulum and Bifidobacterium were poorly enriched. After PSHC intervention, there was a significant reduction in stone size accompanied by decreased serum uric acid and increased urinary pH levels. The abundance of pathogenic bacterium Fusobacterium was significantly downregulated following the intervention, whereas there was an upregulation observed in SCFA-producing bacteria Lachnoclostridium and Parasutterella, leading to a significant elevation in butyric acid content. Functions related to fatty acid synthesis and amino acid metabolism within the microbiota showed upregulation following PSHC intervention. The correlation analysis revealed a positive association between stone pathogenic bacteria abundance and clinical factors for stone formation, while a negative correlation with SCFAs contents. Our preliminary study revealed that alterations in gut microbiota and metabolites were the crucial physiological adaptation to PSHC intervention. Targeted regulation of microbiota and SCFA holds promise for enhancing drug therapy efficacy and preventing stone recurrence.
Key points
• Bacteroides and Fusobacterium were identified as dominant genera for UAS patients
• After PSHC intervention, Fusobacterium decreased and butyric acid content increased
• The microbiota increased capacity for fatty acid synthesis after PSHC intervention
“…5 Furthermore, studies shed light on the hypocholesterolemic effects and antioxidant and anti-inflammatory effects of kefir. 6 Researchers have also found that dairy products fermented by kefir have anti-cancer, antibacterial, anti-allergic, anti-diabetic, anti-inflammatory, antioxidant, and anti-hypertensive effects. 7,8 Additionally, kefir could improve the intestinal tract and digestive system and reduce lactose intolerance.…”
This study utilized high-throughput sequencing and SEM observation to elucidate the microbial composition of TKG-Y, a Tibetan herder's homemade kefir grain. Staphylococcus warneri KYS-164, which could produce bacteriocin-like inhibitory substances...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
