2020
DOI: 10.3390/ani10050758
|View full text |Cite
|
Sign up to set email alerts
|

Milk Metabolomics Reveals Potential Biomarkers for Early Prediction of Pregnancy in Buffaloes Having Undergone Artificial Insemination

Abstract: This study aimed to identify potential biomarkers for early pregnancy diagnosis in buffaloes subjected to artificial insemination (AI). The study was carried out on 10 pregnant and 10 non-pregnant buffaloes that were synchronized by Ovsynch-Timed Artificial Insemination Program and have undergone the first AI. Furthermore, milk samples were individually collected ten days before AI (the start of the synchronization treatment), on the day of AI, day 7 and 18 after AI, and were analyzed by LC–MS. Statistical ana… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

0
8
0

Year Published

2021
2021
2023
2023

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 6 publications
(8 citation statements)
references
References 64 publications
0
8
0
Order By: Relevance
“…At present, metabolomics has been applied to the screening of biomarkers and metabolic changes in various studies, such as heat stress in dairy cows [ 31 ] and pregnancy prediction of buffaloes [ 32 ], while few reports have focused on the metabolic differences between different buffalo species. In this study, we identified 19 and 18 significant different metabolites between the Mediterranean buffalo milk and Murrah or crossbred buffalo milk, respectively.…”
Section: Discussionmentioning
confidence: 99%
“…At present, metabolomics has been applied to the screening of biomarkers and metabolic changes in various studies, such as heat stress in dairy cows [ 31 ] and pregnancy prediction of buffaloes [ 32 ], while few reports have focused on the metabolic differences between different buffalo species. In this study, we identified 19 and 18 significant different metabolites between the Mediterranean buffalo milk and Murrah or crossbred buffalo milk, respectively.…”
Section: Discussionmentioning
confidence: 99%
“…This is also the first study to investigate the untargeted lipidome content of healthy water buffalo milk, to the authors' knowledge. Previous studies focused on the metabolome of milk as an early predictor of pregnancy using the liquid chromatography-mass spectrometry analysis (LC–MS) approach 26 . Other studies examined the effects of nutrition on milk fatty acid profiles using high-resolution gas chromatography (GC) and identified 32 fatty acids 29 , 30 .…”
Section: Discussionmentioning
confidence: 99%
“…After comparing with the closest comparable data set, the bovine milk lipidome 36 , we found that the most abundant bovine milk TAG are also present in water buffalo milk. The two primary fatty acids isolated from water buffalo milk are C16:0 (palmitic acid) and C18:0 (stearic acid), as described 26 , 32 , 33 . Remarkably, the presence of very long-chain fatty acids, namely FA 42:5 and FA 40:5, in milk, is reported here for the first time.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Recent literature indicates promising results when applying metabolomics to detect pregnancy in other livestock species (Fontanesi, 2016;de Nicola et al, 2020;Gómez et al, 2020). However, there are no publications using high throughput metabolomics platforms to characterize non-hormonal metabolite biomarkers that can be used for sheep PLS detection in readily accessible bio uids at early stages of gestation.…”
Section: Introductionmentioning
confidence: 99%