Milk microbiota: Characterization methods and role in cheese production

Tilocca, Bruno; Costanzo, Nicola; Morittu, Valeria Maria; Spina, Anna Antonella; Soggiu, Alessio; Britti, Domenico; Roncada, Paola; Piras, Cristian

doi:10.1016/j.jprot.2019.103534

Cited by 114 publications

(77 citation statements)

References 94 publications

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“…Moreover, a thorough understanding of interspecies communication is of paramount importance for elucidating how environmental factors trigger the resistance mechanisms and their molecular details. In this light, a wide variety of ecological niches harboring complex microbial community are the object of resistome-aimed studies, including the resistome investigation in manure, soil, wastewater, and animal-by food products from a variety of productive processes [3,142,143].…”

Section: Latest Frontiers On Veterinary Amr Researchmentioning

confidence: 99%

“…Cumulative evidence proved the efficacy of bacteriocins also in the food industry. Indigenous microflora of raw milk control pathogenic bacteria through the production of diverse bacteriocins, including Nisin A [143]. Studies evaluating bacteriocins stability in vivo, as well as the most suitable delivery strategy, are now under investigation.…”

Section: Future Perspective For Preventing Insurgence And/or Developmmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial Resistance in Veterinary Medicine: An Overview

Palma

Tilocca

Roncada

2020

IJMS

Self Cite

183

100

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Antimicrobial resistance (AMR) represents one of the most important human- and animal health-threatening issues worldwide. Bacterial capability to face antimicrobial compounds is an ancient feature, enabling bacterial survival over time and the dynamic surrounding. Moreover, bacteria make use of their evolutionary machinery to adapt to the selective pressure exerted by antibiotic treatments, resulting in reduced efficacy of the therapeutic intervention against human and animal infections. The mechanisms responsible for both innate and acquired AMR are thoroughly investigated. Commonly, AMR traits are included in mobilizable genetic elements enabling the homogeneous diffusion of the AMR traits pool between the ecosystems of diverse sectors, such as human medicine, veterinary medicine, and the environment. Thus, a coordinated multisectoral approach, such as One-Health, provides a detailed comprehensive picture of the AMR onset and diffusion. Following a general revision of the molecular mechanisms responsible for both innate and acquired AMR, the present manuscript focuses on reviewing the contribution of veterinary medicine to the overall issue of AMR. The main sources of AMR amenable to veterinary medicine are described, driving the attention towards the indissoluble cross-talk existing between the diverse ecosystems and sectors and their cumulative cooperation to this warning phenomenon.

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Section: Latest Frontiers On Veterinary Amr Researchmentioning

confidence: 99%

Section: Future Perspective For Preventing Insurgence And/or Developmmentioning

confidence: 99%

Antimicrobial Resistance in Veterinary Medicine: An Overview

Palma

Tilocca

Roncada

2020

IJMS

Self Cite

183

100

View full text Add to dashboard Cite

show abstract

“…Besides high throughput marker gene or shotgun sequencing, other omics approaches, as reviewed by Tilocca et al [1], have been used to characterize the microbiota of dairy milk and products. Whether they involve gene expression analyses of microbial communities (metatranscriptomics), identification and quantification (meta-metabolomics) of their metabolites or proteome analysis (metaproteomics), they all depend on microbiome stability for consistent and reliable study results.…”

Section: Discussionmentioning

confidence: 99%

“…The interest in culture-independent approaches to study the microbiological quality of dairy milk has continued to rise, especially with the advent of high-throughput omics technologies in the field of dairy science and technology, including metatranscriptomics, metaproteomics, and metagenomics [1]. Closely related to the logistics of implementing farming practices across the dairy production chain, microorganisms found in bulk tank milk have been shown to accurately typify dairy farms and the various inherent sources of contamination [2,3].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Preservation Methods Allows Deeper Insights into Changes of Raw Milk Microbiota

et al. 2020

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The temporal instability of raw milk microbiota drastically affects the reliability of microbiome studies. However, little is known about the microbial integrity in preserved samples. Raw cow milk samples were preserved with azidiol or bronopol and stored at 4 °C, or with dimethyl sulfoxide (DMSO) or a mixture of azidiol and DMSO and stored at −20 °C for up to 30 days. Aliquots of 5-, 10-, and 30-day post-storage were treated with propidium monoazide (PMA), then analyzed by sequencing the 16S rRNA gene V3-V4 and V6-V8 regions. The V6-V8 gave a higher richness and lower diversity than the V3-V4 region. After 5-day storage at 4 °C, the microbiota of unpreserved samples was characterized by a drastic decrease in diversity, and a significant shift in community structure. The treatment with azidiol and DMSO conferred the best community stabilization in preserved raw milk. Interestingly, the azidiol treatment performed as well for up to 10 days, thus appearing as a suitable alternative. However, neither azidiol nor bronopol could minimize fungal proliferation as revealed by PMA-qPCR assays. This study demonstrates the preservative ability of a mixture of azidiol and DMSO and provides deeper insights into the microbial changes occurring during the cold storage of preserved raw milk.

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“…The typical metaproteomics workflow is shown in Figure 3, including (a) extraction and purification of proteins from TFFB; (b) protein digestion; (c) mass spectrometry (MS) analysis of peptide fragments; (d) protein qualitative and quantitative analysis (Schneider & Riedel, 2010; Tilocca et al., 2020). The qualitative properties of proteins can be retrieved through a non‐assembled metagenome–derived database, an assembled metagenome–derived database, or a taxonomy–built database (Geron, Werner, Wattiez, Lebaron, & Matallana‐Surget, 2019).…”

Section: Metaproteomics Analysis Methodsmentioning

confidence: 99%

Metaproteomics insights into traditional fermented foods and beverages

Yang

Fan

2020

Comp Rev Food Sci Food Safe

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Traditional fermented foods and beverages (TFFB) are important dietary components. Multi‐omics techniques have been applied to all aspects of TFFB research to clarify the composition and nutritional value of TFFB, and to reveal the microbial community, microbial interactions, fermentative kinetics, and metabolic profiles during the fermentation process of TFFB. Because of the advantages of metaproteomics in providing functional information, this technology has increasingly been used in research to assess the functional diversity of microbial communities. Metaproteomics is gradually gaining attention in the field of TFFB research because it can reveal the nature of microorganism function at the protein level. This paper reviews the common methods of metaproteomics applied in TFFB research; systematically summarizes the results of metaproteomics research on TFFB, such as sauces, wines, fermented tea, cheese, and fermented fish; and compares the differences in conclusions reached through metaproteomics versus other omics methods. Metaproteomics has great advantages in revealing the microbial functions in TFFB and the interaction between the materials and microbial community. In the future, metaproteomics should be further applied to the study of functional protein markers and protein interaction in TFFB; multi‐omics technology requires further integration to reveal the molecular nature of TFFB fermentation.

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Milk microbiota: Characterization methods and role in cheese production

Cited by 114 publications

References 94 publications

Antimicrobial Resistance in Veterinary Medicine: An Overview

Antimicrobial Resistance in Veterinary Medicine: An Overview

Optimization of Preservation Methods Allows Deeper Insights into Changes of Raw Milk Microbiota

Metaproteomics insights into traditional fermented foods and beverages

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