Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses

López‐Gatius, F.; Garbayo, J. M.; Santolaria, P.; Yániz, J.L.; Ayad, Abdelhanine; Sousa, Noelita Melo de; Beckers, J. F.

doi:10.1016/j.domaniend.2005.12.007

Cited by 83 publications

(93 citation statements)

References 44 publications

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“…PAG-1 concentrations were determined in plasma using a double antibody radioimmunoassay procedure (RIA-706) [29]. Rabbit polyclonal antiserum AS#706 was raised against caprine PAG 55 kDaþ62 kDa (accession numbers P80935 and P80933) according to Vaitukaitis et al [30].…”

Section: Endocrine Determinationsmentioning

confidence: 99%

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Effects of crossbreeding on endocrine patterns determined in pregnant beef/dairy cows naturally infected with Neospora caninum

et al. 2015

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Section: Endocrine Determinationsmentioning

confidence: 99%

“…P4 concentrations were determined in plasma using a direct method (without extraction) as previously described in detail [29]. The minimum detection limit of the P4-RIA technique used is 0.15 ng/mL and intra-assay and interassay coefficients of variation are 5.1% and 10.5%, respectively.…”

Section: Endocrine Determinationsmentioning

confidence: 99%

Effects of crossbreeding on endocrine patterns determined in pregnant beef/dairy cows naturally infected with Neospora caninum

et al. 2015

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“…Since it is difficult to obtain and process blood samples in the field, attempts have been made to identify pregnancy specific proteins in other biological fluids, such as urine and milk [16]. These studies found, however, that measurements of PSPB and PAG concentrations in milk were inadequate for diagnosing pregnancy [17][18][19][20]. Proteomics approaches were employed for the study on mastitis milk or identification of small milk proteins and fatbound proteins [21][22][23][24] and in recent years milk proteomes were pursued for human and animal milk protein differences [25].…”

Section: Introductionmentioning

confidence: 98%

Detection of early pregnancy-specific proteins in Holstein milk

Han

Kim

Diao

et al. 2012

Journal of Proteomics

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“…Plasma was immediately separated by centrifugation (5 000 g) and stored frozen at -20 °C. Progesterone (P4) concentrations were determined by direct radioimmunoassay (RIA) method (without extraction), as described in detail by Lopez-Gatius et al (2007). The minimum detection limit of the P4-RIA technique used was 0.15 ng/ml.…”

Section: Animals Treatment and Ultrasound Schedulementioning

confidence: 99%

Determination of minimum effective doses of luteinizing hormone and human chorionic gonadotropin for intrafollicular treatment to induce ovulation in dairy heifers

et al. 2013

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The aim of this study was to determine the minimum effective intrafollicular doses of luteinizing hormone and human chorionic gonadotropin in order to induce ovulation in cycling dairy heifers that have not yet been adequately established. Application of 10, 5, 1, 0.5, 0.1, 0.01 and 0.001 µg luteinizing hormone as well as 10, 1, 0.1, 0.01 and 0.001 international units (IU) of human chorionic gonadotropin in dominant follicles was performed on day 7 of the oestrous cycle. Control animals were given luteinizing hormone (12.5 mg and 25 mg) or human chorionic gonadotropin (2000 IU) intravenously. Accessory corpus luteum on day 14 of the oestrous cycle was considered as an evidence of ovulation. Ovulation was observed in 2 out of 3 heifers in each treatment group (n = 3) after administration of 10-0.1 µg luteinizing hormone (except for 0.5 µg -ovulation in 3 of 3 heifers), in all heifers after administration of 10-0.01 IU human chorionic gonadotropin as well as in all control heifers. Administration of 0.01 µg and 0.001 µg luteinizing hormone as well as of 0.001 IU human chorionic gonadotropin did not result in ovulation. Higher progesterone concentration on day 14 vs. day 7 of the oestrous cycle was found after all treatments. Nevertheless, the differences were significant (P < 0.05) only after intrafollicular treatments with 5, 1 and 0.001 µg luteinizing hormone as well as 10, 1 and 0.01 IU human chorionic gonadotropin. In conclusion, minimum efficient doses for intrafollicular treatment of the dominant follicles in cycling heifers capable of inducing ovulation were 0.1 µg of luteinizing hormone and 0.01 IU of human chorionic gonadotropin. This is the first study describing the intrafollicular luteinizing hormone administration in cycling dairy heifers. Intraovarian injection, ovum pick-up, ultrasound-guided transvaginal ovarian puncture, accessory corpus luteumUltrasound-guided transvaginal intrafollicular injection of human chorionic gonadotropin (hCG) in cattle was first reported as a useful research tool by Kot et al. in 1995. Since then, intrafollicular injection of different substances such as phosphate-buffered saline or insulin-like growth factor-I (Bergfelt et al. 1998;Ginther et al. 2004;Shahiduzzaman et al. 2010) as well as intrafollicular insemination (Lopez-Gatius and Hunter 2011) have been described. In our former study, intrafollicular treatment (IFT) with luteinizing hormone (LH) injected in the dominant follicle in heifers previously treated by deslorelin implants was reported. Ovulation was proven after the intrafollicular treatment using various doses of LH . However, minimum effective doses of LH for IFT in comparison with IFT using hCG have not yet been adequately established.The aim of this study was to determine the minimum efficient doses of LH and hCG for intrafollicular treatment to induce ovulation in cycling dairy heifers. Materials and Methods Intrafollicular treatment equipmentIntrafollicular treatment was performed using a newly developed double channel instrument enabling asp...

show abstract

Milk production correlates negatively with plasma levels of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses

Cited by 83 publications

References 44 publications

Effects of crossbreeding on endocrine patterns determined in pregnant beef/dairy cows naturally infected with Neospora caninum

Effects of crossbreeding on endocrine patterns determined in pregnant beef/dairy cows naturally infected with Neospora caninum

Detection of early pregnancy-specific proteins in Holstein milk

Determination of minimum effective doses of luteinizing hormone and human chorionic gonadotropin for intrafollicular treatment to induce ovulation in dairy heifers

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