Milk proteins: precursors of bioactive peptides

Meisel, Hans; Schlimme, E.

doi:10.1016/0924-2244(90)90029-x

Cited by 118 publications

(64 citation statements)

References 25 publications

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“…Casein phosphopeptides (CPPs) were well known for their metal binding abilities because the CPPs contained electronegative phosphoserines (West et al, 1986;Meisel et al, 1990;Yuan et al, 1991;Gerber et al, 1986;Sato et al, 1986). The phosphopeptides obtained from the enzymolysis of fish bone (Jung et al, 2007(Jung et al, , 2006(Jung et al, , 2005 can also chelate Ca, whose performance is similar to CPPs.…”

Section: Influenced By Lactose Intolerance Dietary Habits and Othermentioning

confidence: 99%

Purification of a Novel Oligophosphopeptide with High Calcium Binding Activity from Carp Egg Hydrolysate

Huang

Liu

et al. 2014

FSTR

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Keywords: purification, carp eggs, hydrolysate, dephosphorization, calcium binding activity, oligophosphopeptide IntroductionThe global fishery production reached 178 million tons in 2011(i), more than 50% of which was discarded as inedible byproducts such as bone, skin, internal organs, head and eggs (Jung et al., 2005). Plentiful research have been implemented on utilizing fishery byproducts (Chakraborty et al., 2011;Duan et al., 2009;Kongsri et al., 2013;Mori et al., 2013). However, the utilization studies on eggs, especially on freshwater fish eggs, are very scarce.Eggs are rich in lipids (especially phospholipids and n-3 polyunsaturated fatty acids), the yolk protein, mineral elements, vitamins, carotenoids, and other nutrients. But in the conventional process, eggs are often treated as waste to be abandoned. AbbreviationsCPP; casein phosphopeptide, Hy; carp egg tryptic hydrolysate, DP0; carp eggs without dephosphorization, DP1, DP2, DP3, and DP4; dephosphorized carp eggs with degree of dephosphorization of 13.66%, 30.39%, 48.71%, and 83.49% respectively, U1 and U2; the fractions after ultrafiltration with molecular weight <3 kDa and > 3 kDa respectively, H1, H2 and H3; the fractions eluted with 100 mM, 200 mM and 400 mM phosphate buffer respectively on hydroxyapatite chromatography column, HA; hydroxyapatite chromatography H. Huang et al. 800 Calcium is an important mineral element to maintain human health, which plays an important role in the formation of bones and teeth, neurotransmitter release, muscle composition, and heart beat regulation. Calcium is mainly ingested through drinks and food, especially milk or cheese products with high calcium content. Influenced by lactose intolerance, dietary habits, and other factors,Asians intake less milk and cheese products and their food mainly focuses on vegetable diet. However, substances such as the phytic acid, oxalic acid, saturated fatty acids, and dietary fiber are easily combined with calcium to form insoluble matter, which is not beneficial to calcium absorption.Many peptides isolated from animals possessed the ability of binding Ca Lee et al., 2009;Jiang et al., 2000;Jung et al., 2007). Casein phosphopeptides (CPPs) were well known for their metal binding abilities because the CPPs contained electronegative phosphoserines (West et al., 1986;Meisel et al., 1990;Yuan et al., 1991;Gerber et al., 1986;Sato et al., 1986). The phosphopeptides obtained from the enzymolysis of fish bone (Jung et al., 2007(Jung et al., , 2006(Jung et al., , 2005 can also chelate Ca, whose performance is similar to CPPs. Many studies indicated that fish eggs also contain high phosphoprotein and can obtain phosphopeptides by hydrolysis (Catherine et al., 2002;Inoue et al., 1971).Carp was one of the most productive (over 3.8 million tons ) freshwater fish species in the world (ii). In breeding season, the ovarian can weigh 10% to 25.81% of the overall weight. In the present study, we will discuss the appropriate degree of dephosphorization of carp eggs before trypsin hydrolysi...

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Section: Influenced By Lactose Intolerance Dietary Habits and Othermentioning

confidence: 99%

Purification of a Novel Oligophosphopeptide with High Calcium Binding Activity from Carp Egg Hydrolysate

Huang

Liu

et al. 2014

FSTR

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“…milk and gluten). These peptides have been shown to influence intestinal function and some evidence indicates that they might be absorbed and then affect the central nervous system (Morley et al 1983;Gardner, 1984;Meisel & Schlimme, 1990).…”

Section: H O C O L a T Ementioning

confidence: 99%

Food, Mood and Appetite

Rogers

1995

Nutr. Res. Rev.

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“…Casein hydrolysates offer the advantage of providing the amino acid content of milk caseins in a predigested form which facilitates its intestinal absorption. However, since specific casein sequences have been identified as potential bioactive peptides such as opioids and minerai carriers (Meisel and Schlimme, 1990), downstream fractionation steps have been proposed (Touraine et al, 1987). Brulé et al (1980) have patented a process for the preparation of caseinophosphopeptides using ultrafiltration (UF) membranes under specifie physico-chemical conditions, namely pH 6.2 and with 0.2% Na2HP04 and 0.5% CaCI2 added to the hydrolysate prior to UF.…”

Section: Introductionmentioning

confidence: 99%

Separation of casein hydrolysates using polysulfone ultrafiltration membranes with pH and EDTA treatments applied

Gourley

Gauthier

Pouliot

1995

Lait

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Summary -The preparation of peptide fractions from case in hydrolysates can be achieved by the use of ultrafiltration for the removal of the enzyme from the reaction mixture and/or to obtain specifie fractions of peptides. However, the ultrafiltration of case in hydrolysates is subjected to severe fouling phenomena which affect both the flux decline and the rejection properties of the membrane. Polysulfone membrane material used in previous work showed specifie rejection properties towards charged or hydroxylated amino acids. Polysulfone and polyethersulfone fiat sheet membranes were selected for their distinctive surface properties. The ultrafiltration of a tryptic hydrolysate from sodium caseinate was studied and physico-chemical variables were introduced, namely, pH of the hydrolysate (pH 6, 8, 10) and addition of a calcium sequestrant (EDTA 20 mmol 1-1 ). Flux decline measurements, total nitrogen rejection, molecular mass distribution profile, and amino acid composition of the permeates were determined. Although the polyethersulfone membranes showed greater permeability to water (+ 15%) at 25°C th an polysulfone, no distinctive effect of membrane material was observed in the permeation flux (1 h-1 m-2 ) during ultrafiltration of the hydrolysates. The rejection coefficients were similar for both materials. It was observed that the pH and the excess of EDT A had a much stronger effect th an the mate rial on the rejection properties (nitrogen, peptides, amino acids) and the flux decline. The two materials seemed to exhibit different surface reactivity towards charged molecules.

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Milk proteins: precursors of bioactive peptides

Cited by 118 publications

References 25 publications

Purification of a Novel Oligophosphopeptide with High Calcium Binding Activity from Carp Egg Hydrolysate

Purification of a Novel Oligophosphopeptide with High Calcium Binding Activity from Carp Egg Hydrolysate

Food, Mood and Appetite

Separation of casein hydrolysates using polysulfone ultrafiltration membranes with pH and EDTA treatments applied

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