Milk sphingomyelin improves lipid metabolism and alters gut microbiota in high fat diet-fed mice

Norris, Gregory H; Jiang, Christina; Ryan, Julia; Porter, Caitlin M.; Blesso, Christopher N.

doi:10.1016/j.jnutbio.2015.12.003

Cited by 141 publications

(146 citation statements)

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“…Sphingosine, a hydrolytic product of dietary SM, is known to have bactericidal effects [53]. We have previously reported that supplementation of 0.25% (w/w) milk SM to HFD-fed mice altered the fecal microbiota composition by reducing Gram-negative bacterial phyla and increasing Bifidobacterium [28]. In the current study, however, we did not observe such large changes in gut microbiota composition, possibly related to the lower dose used or the more severe HFD challenge.…”

Section: Discussioncontrasting

confidence: 88%

“…Lower serum CCL2 and mRNA expression of inflammation markers in epididymal adipose tissue were also previously shown in mice fed milk SM [29]. We have previously reported that the 4-week dietary supplementation of a higher dose of milk SM (0.25% w/w) reduced circulating LPS by 35% in mice fed a milkfat-based high-fat diet [28]. Although we did not observe any differences in mRNA expression of gut barrier markers in the small intestine, reductions in circulating LPS are expected to be partly due to the inhibitory effect of milk SM on lipid absorption.…”

Section: Discussionsupporting

confidence: 63%

“…Although we did not observe any differences in mRNA expression of gut barrier markers in the small intestine, reductions in circulating LPS are expected to be partly due to the inhibitory effect of milk SM on lipid absorption. Supporting this notion, we have previously observed reductions in serum and hepatic lipids with the feeding of milk SM [28,29].…”

Section: Discussionmentioning

confidence: 54%

“…The presence of LPS in circulation also results in the liver upregulating sphingolipid biosynthesis via the sphingolipid rate-limiting biosynthetic enzyme, serine palmitoyltransferase (SPT), possibly as a compensatory mechanism [27]. We have previously shown that feeding 0.25% (w/w) milk SM reduced serum LPS and altered the gut microbiota in HFD-fed mice after four weeks [28]. We also recently reported that feeding dietary SM (0.1% w/w) attenuated hepatic steatosis and adipose tissue inflammation in diet-induced obese mice [29].…”

Section: Introductionmentioning

confidence: 99%

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Dietary Milk Sphingomyelin Reduces Systemic Inflammation in Diet-Induced Obese Mice and Inhibits LPS Activity in Macrophages

et al. 2017

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High-fat diets (HFD) increase lipopolysaccharide (LPS) activity in the blood and may contribute to systemic inflammation with obesity. We hypothesized that dietary milk sphingomyelin (SM), which reduces lipid absorption and colitis in mice, would reduce inflammation and be mediated through effects on gut health and LPS activity. C57BL/6J mice were fed high-fat, high-cholesterol diets (HFD, n = 14) or the same diets with milk SM (HFD-MSM, 0.1% by weight, n = 14) for 10 weeks. HFD-MSM significantly reduced serum inflammatory markers and tended to lower serum LPS (p = 0.08) compared to HFD. Gene expression related to gut barrier function and macrophage inflammation were largely unchanged in colon and mesenteric adipose tissues. Cecal gut microbiota composition showed greater abundance of Acetatifactor genus in mice fed milk SM, but minimal changes in other taxa. Milk SM significantly attenuated the effect of LPS on pro-inflammatory gene expression in RAW264.7 macrophages. Milk SM lost its effects when hydrolysis was blocked, while long-chain ceramides and sphingosine, but not dihydroceramides, were anti-inflammatory. Our data suggest that dietary milk SM may be effective in reducing systemic inflammation through inhibition of LPS activity and that hydrolytic products of milk SM are important for these effects.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Dietary Milk Sphingomyelin Reduces Systemic Inflammation in Diet-Induced Obese Mice and Inhibits LPS Activity in Macrophages

et al. 2017

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“…D203, Page 7 de 12 terme protéger de l'inflammation métabolique induite notamment par l'absorption d'endotoxines comme expliqué plus haut ; une étude récente chez la souris a par ailleurs montré que la sphingomyéline laitière peut contribuer à diminuer l'endotoxémie métabolique suite à un régime hyperlipidique chez la souris (Norris et al, 2016). Dans la poursuite de l'étude du devenir métabolique des lipides, il deviendra ainsi crucial de s'intéresser également aux effets de résidus lipidiques non absorbés au cours de la digestion, atteignant le côlon et y exerçant des effets métaboliques spécifiques.…”

Section: Topical Issueunclassified

Impacts métaboliques et inflammatoires des matières grasses émulsionnées

Michalski

Vors

Lecomte

et al. 2017

Cahiers de Nutrition et de Diététique

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Reçu le 31 janvier 2017 -Accepté le 2 mars 2017Résumé -Les effets des lipides sur la santé doivent aujourd'hui être étudiés par des approches allant au-delà de leur densité énergétique et de leur profil en acides gras. En effet, ces acides gras sont les briques élémentaires de différentes molécules lipidiques telles que les triacylglycérols et les phospholipides, elles-mêmes organisées sous forme de structures supramoléculaires variées comme les gouttelettes d'émulsion, et pouvant être intégrées dans différentes matrices alimentaires. Ce court article résume nos récents travaux sur l'impact de l'état émulsionné de la matière grasse sur le métabolisme post-prandial des lipides et la b-oxydation des acides gras ingérés chez l'homme mince ou obèse, ouvrant la voie au concept de « lipides rapides versus lipides lents ». Nous présentons comment la cinétique post-prandiale d'absorption des lipides peut aussi contribuer à moduler l'endotoxémie métabolique, provenant en partie d'interactions entre les lipides alimentaires et le microbiote intestinal et pouvant contribuer à l'inflammation métabolique chez l'obèse. Enfin, nous mettons en exergue l'impact pro-ou anti-inflammatoire chez la souris d'agents tensioactifs utilisés en formulation alimentaire pour stabiliser les émulsions, notamment les phospholipides d'origine végétale ou laitière, ainsi que de différents vecteurs moléculaires d'acides gras poly-insaturés n-3 à longue chaîne. Le lecteur pourra se référer à nos autres publications et revues récentes pour un approfondissement des concepts présentés.Mots clés : nutrition / matière grasse / huile / émulsifiant / inflammation / oxydation / phospholipide Abstract -Metabolic and inflammatory impacts of emulsified fat. The health effects of lipids must now be explored beyond their energy content and fatty acid profile. Indeed, fatty acids as unit elements of different molecules such as triacylglycerols and phospholipids are being organized in various supramolecular structures such as emulsion droplets, and incorporated in complex food matrixes. This short article reviews our recent studies on the impact of fat emulsified structure on postprandial lipid metabolism and fatty acid beta-oxidation in normal-weight and obese humans, leading to the concept of "fast versus slow lipids". We also show how the postprandial kinetics of lipid absorption can contribute to modulate metabolic endotoxemia, partly arising from interactions between dietary lipids and gut microbiota, and able to contribute to metabolic inflammation in obesity. Finally, we highlight the pro-or antiinflammatory impact in mice of surface active agents used in food formulation to stabilize emulsions, notably phospholipids of vegetal or dairy origin, and different molecular carriers of long-chain n-3 polyunsaturated fatty acids. The interested reader will refer to our other recent publications and reviews on these topics for a deeper insight into presented concepts.

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Host–diet–microbiota interplay in intestinal nutrition and health

Ignatiou,

Pitsouli

2024

FEBS Letters

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The intestine is populated by a complex and dynamic assortment of microbes, collectively called gut microbiota, that interact with the host and contribute to its metabolism and physiology. Diet is considered a key regulator of intestinal microbiota, as ingested nutrients interact with and shape the resident microbiota composition. Furthermore, recent studies underscore the interplay of dietary and microbiota‐derived nutrients, which directly impinge on intestinal stem cells regulating their turnover to ensure a healthy gut barrier. Although advanced sequencing methodologies have allowed the characterization of the human gut microbiome, mechanistic studies assessing diet–microbiota–host interactions depend on the use of genetically tractable models, such as Drosophila melanogaster. In this review, we first discuss the similarities between the human and fly intestines and then we focus on the effects of diet and microbiota on nutrient‐sensing signaling cascades controlling intestinal stem cell self‐renewal and differentiation, as well as disease. Finally, we underline the use of the Drosophila model in assessing the role of microbiota in gut‐related pathologies and in understanding the mechanisms that mediate different whole‐body manifestations of gut dysfunction.

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Milk sphingomyelin improves lipid metabolism and alters gut microbiota in high fat diet-fed mice

Cited by 141 publications

References 54 publications

Dietary Milk Sphingomyelin Reduces Systemic Inflammation in Diet-Induced Obese Mice and Inhibits LPS Activity in Macrophages

Dietary Milk Sphingomyelin Reduces Systemic Inflammation in Diet-Induced Obese Mice and Inhibits LPS Activity in Macrophages

Impacts métaboliques et inflammatoires des matières grasses émulsionnées

Host–diet–microbiota interplay in intestinal nutrition and health

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