Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Bhardwaj, Nandana; Rajkhowa, Rangam; Wang, Xungai; Devi, Dipali

doi:10.1016/j.ijbiomac.2015.07.049

Cited by 46 publications

(30 citation statements)

References 61 publications

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“…This phenomenon might be due to the fact that the particles entering the cell have a certain critical radius that is less than the large size of microparticles, causing microparticles to be excluded from the cell membrane [ 7 ]. The results were similar to those of Nandana [ 56 ], who reported that mouse fibroblast cells L929 were adhered to the surface of SFMPs with diameters of 4–5μm. In contrast, cells internalize nanoparticles, which involves a binding (adsorbing) process followed by the formation of vesicles, and then, the formed nanoparticle-containing vesicles are internalized by endocytosis [ 11 ].…”

Section: Discussionsupporting

confidence: 90%

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

et al. 2020

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When silk fibroin particles are used for controlled drug delivery, particle size plays a key role in the location of the carrier on the cells as well as the transport pathway, utilization efficiency, and therapeutic effect of the drugs. In this study, the interactions of different-sized silk fibroin particles and cell lines were investigated. Silk fibroin microparticles with dry size of 1.9 ± 0.4 μm (2.7 ± 0.3 μm in wet state) and silk fibroin nanoparticles with dry size of 51.5 ± 11.0 nm (174.8 ± 12.5 nm in wet state) were prepared by salting-out method and high-voltage electrospray method, respectively. CdSe/ZnS quantum dots were coupled to the surface of the micro/nanoparticles. Photostability observations indicated that the fluorescence stability of the quantum dots was much higher than that of fluorescein isothiocyanate. In vitro, microparticles and nanoparticles were co-cultured with human umbilical vein endothelial cells EA.hy 926 and cervical cancer cells HeLa, respectively. The fluorescence test and cell viability showed that the EA.hy926 cells tended to be adhered to the microparticle surfaces and the cell proliferation was significantly promoted, while the nanoparticles were more likely to be internalized in HeLa cells and the cell proliferation was notably inhibited. Our findings might provide useful information concerning effective drug delivery that microparticles may be preferred if the drugs need to be delivered to normal cell surface, while nanoparticles may be preferred if the drugs need to be transmitted in tumor cells.

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Section: Discussionsupporting

confidence: 90%

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

et al. 2020

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“…Also, alternative choices to B. mori silk are the non-mulberry tropical silkworm silks such as tasar ( Antheraea mylitta ) silk, which possesses higher mechanical strength and better cell adhesion due to the presence of Arg-Gly-Asp (RGD) recognition sequences [ 1 ]. With enhanced cell attachment and tunable degradation, non-mulberry silk fibroins can be employed in various forms of biomaterials, such as SF microparticles for drug delivery vehicles or microcarriers using wet-milling and spray drying techniques [ 4 ]. The biomedical and therapeutic significance of SF derivatives and matrices have been the subject of extensive study over the past several years.…”

Section: Introductionmentioning

confidence: 99%

Tissue Regeneration: A Silk Road

Jao

Mou

2016

JFB

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Silk proteins are natural biopolymers that have extensive structural possibilities for chemical and mechanical modifications to facilitate novel properties, functions, and applications in the biomedical field. The versatile processability of silk fibroins (SF) into different forms such as gels, films, foams, membranes, scaffolds, and nanofibers makes it appealing in a variety of applications that require mechanically superior, biocompatible, biodegradable, and functionalizable biomaterials. There is no doubt that nature is the world’s best biological engineer, with simple, exquisite but powerful designs that have inspired novel technologies. By understanding the surface interaction of silk materials with living cells, unique characteristics can be implemented through structural modifications, such as controllable wettability, high-strength adhesiveness, and reflectivity properties, suggesting its potential suitability for surgical, optical, and other biomedical applications. All of the interesting features of SF, such as tunable biodegradation, anti-bacterial properties, and mechanical properties combined with potential self-healing modifications, make it ideal for future tissue engineering applications. In this review, we first demonstrate the current understanding of the structures and mechanical properties of SF and the various functionalizations of SF matrices through chemical and physical manipulations. Then the diverse applications of SF architectures and scaffolds for different regenerative medicine will be discussed in detail, including their current applications in bone, eye, nerve, skin, tendon, ligament, and cartilage regeneration.

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“…FTIR and XRD analyses of the SF nanofibers are collectively presented in Figure . FTIR spectra of the pure and MNPs‐containing SF nanofiber mats (M1 and M2) showed the characteristic absorption peaks of β ‐sheet conformation of the silk fibroin polymer around 1620 cm −1 (CO stretching), 1520 cm −1 (NH bending and CH stretching), and 1240 cm −1 (CN stretching and CO bending) …”

Section: Resultsmentioning

confidence: 99%

Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties

Lalegül‐Ülker

Vurat

Elçin

et al. 2019

J of Applied Polymer Sci

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Magnetically responsive polymer composites have great potential for use in diverse biomedical applications. In this study, composite biomaterials consisting of silk fibroin (SF) and superparamagnetic iron oxide nanoparticles (SPIONs) were fabricated by the electrospinning method. Two different methods were employed to incorporate the SPIONs into the SF nanofibers. In the first encapsulation method (M1), SPIONs (1.0, 3.0, and 5.0 wt%) were initially included in the electrospinning solution. In the second dip-coating method (M2), electrospun SF nanofiber mats were immersed in the aqueous suspensions of SPIONs (10, 30 and 50% v/v). Then, the pure and composite silk fibroin composite mats were comparatively evaluated for their morphological, chemical, magnetic, mechanical and in vitro biological properties, by using a number of methods including SEM, TEM, FTIR, XRD, EDS, VSM, TGA, mechanical tensile tests, as well as by indirect in vitro cytotoxicity and in vitro hemocompatibility analyses. Overall findings suggested that, while M1 nanofiber mats could be a suitable candidate for use in tissue engineering as a magnetically responsive cytocompatible scaffold, the M2 nanofiber mats perhaps could be more appropriate as an interface for triggering the in vitro stem cell differentiation and/or biosensor applications.

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Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Cited by 46 publications

References 61 publications

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

The Interactions of Quantum Dot-Labeled Silk Fibroin Micro/Nanoparticles with Cells

Tissue Regeneration: A Silk Road

Magnetic silk fibroin composite nanofibers for biomedical applications: Fabrication and evaluation of the chemical, thermal, mechanical, and in vitro biological properties

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