The oasis villages of the Tarim Basin served as hubs along the ancient Silk Road, and they played an important role in facilitating communication between the imperial centers of Asia. These villages were supported by an irrigated form of cereal farming that was specifically adapted to these early oasis settlements. In this manuscript, we present the results from new archaeobotanical analyses, radiocarbon dating, and organic carbon isotopic studies directly from carbonized seeds at the Wupaer site (1500-400 BC) in the Kashgar Oasis of the western Tarim Basin. Our results showed that early farming in the oasis relied on a mixed wheat and barley system, but after 1200 BC was intensified through more elaborate irrigation, the introduction of more water-demanding legumes, and possibly a greater reliance on free-threshing wheat. These crops and the knowledge of irrigated farming likely dispersed into the Tarim Basin through the mountains from southern Central Asia. Improved agricultural productivity in the Tarim Basin may also have led to demographic and socio-political shifts and fed into the increased exchange that is colloquially referred to as the Silk Road. The Tarim Basin is located at the central point of the historic Eurasia trade routes; resting in the rain shadows of the Kunlun and Tianshan mountains, it is one of the driest and least arable areas in Asia. The Taklimakan Desert occupies the middle of the basin, and while receiving almost no rainfall, it evaporates up to 3,000 mm per annum. Glacial-melt streams from the mountains flow into the desert and mostly disappear in its hinterland. This landscape gave birth to the beaded oases that characterized the historic Silk Road. Archaeological and historical evidence attests to the presence of agricultural city-states or politically organized villages in these oases, likely being founded as early as the fifth century BC 1. Archaeologists have suggested that many of these urbanized oases housed populations of thousands to tens of thousands, with some notable examples including Qiuci, Yumi, Yanqi, and Loulan (Fig. 1) 2,3. Historians refer to these pockets of dense human occupation as the "Western Regions" 2,3. Prehistorically, these oases played a prominent role in cultural exchange and diffusion during the early globalization process 4-10. These city-states helped connect the two ends of ancient Eurasia and were the main channel between peoples in the regions of northern China and southwest Asia 11-14. For decades, archaeologists have recognized cultural similarities between ancient peoples in the Tien Shan Mountains, Pamirs Plateau, Ferghana Basin, and those that lived across the oases of Xinjiang 12,15,16. The study of the origin and evolution of these city-states, their cultural adaptations, and their response to climate change has been the focus of global historians, archaeologists, and paleoclimate scholars 9,17. Since the Swedish archaeologists, Bergman, first found the well-preserved mummies of the Xiaohe Cemetery in Lop Nur in 1934 18 , new prehistoric se...