Milligrams to kilograms: making microbes work at scale

Cordell, William T.; Avolio, G.; Pfleger, Brian F.

doi:10.1016/j.tibtech.2023.05.002

Cited by 13 publications

(5 citation statements)

References 101 publications

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“…Like yeasts, oleaginous fungi catabolize lipids via the peroxisomal β oxidation pathway. To achieve profitable production of lipids, it is essential to effectively utilize pretreated waste to attain high growth rates, productivity, and yields (Cordell et al, 2023). The single-cell factories must resist possible inhibitors present in these carbon sources and contamination in industrial scales, and downstream purification must be as consistent and effective as possible to be considered a possible producing strain (Park and Ledesma-Amaro, 2023).…”

Section: Microbial Lipid Biosynthesis From Lignocellulosic Hydrolysatementioning

confidence: 99%

New trends in microbial lipid-based biorefinery for fermentative bioenergy production from lignocellulosic biomass

Al Azad,

Madadi,

Song

et al. 2024

Biofuel Res. J.

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Using oleaginous microbial lipid-based biorefinery from lignocellulosic biomass (LCB) to produce fermentative bioenergy (i.e., biodiesel) represents an innovative second-generation fuel production technology. These lipids are predominantly intracellular triglycerides that accumulate through the metabolism of sugars in fermentation following pretreatment and enzymatic hydrolysis of LCB. This review investigates the recent advances in the microbial lipid production from LCB, focusing on the factors influencing the lead microbial lipid producers, different pretreatment methods (i.e., physical, chemical, biological, and combined pretreatment), enzymatic hydrolysis approaches, novel bioprocessing strategies (i.e., microbes-specific and fermentation model specific), and engineering techniques of the oleaginous microbes (i.e., genetic and metabolic alterations). The study demonstrates that oleaginous yeasts can synthesize significantly higher quantities of lipids when incorporated into the system, known as separated hydrolysis and lipid production, following various combined pretreatment methods. Interestingly, CRISPR is found to be the most suitable way of engineering microbes genetically and metabolically for increased lipid synthesis. The study also explores economically viable strategies for fermentative lipid production, addressing associated challenges, and outlines future directions, including comprehensive techno-economic and life cycle assessments. This review offers invaluable insights into microbial lipid production from LCB, highlighting the potential for significant technological and environmental enhancements through ongoing research and development efforts.

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Section: Microbial Lipid Biosynthesis From Lignocellulosic Hydrolysatementioning

confidence: 99%

New trends in microbial lipid-based biorefinery for fermentative bioenergy production from lignocellulosic biomass

Al Azad,

Madadi,

Song

et al. 2024

Biofuel Res. J.

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“…Microorganisms are crucial in biotechnology for producing bulk chemicals, proteins, biofuels, and pharmaceuticals. During fermentation, these microbes often face stress conditions, such as pH variations, changing temperature, and nutrient levels (Cordell et al 2023 ). Media components can significantly contribute to the overall cost of a bioprocess (Cardoso et al 2020 ), and phosphate is a scarce and costly component.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Escherichia coli abiotic stress resistance through ornithine lipid formation

Bedoya-Pérez,

Aguilar-Vera,

Sánchez-Pérez

et al. 2024

Appl Microbiol Biotechnol

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Escherichia coli is a common host for biotechnology and synthetic biology applications. During growth and fermentation, the microbes are often exposed to stress conditions, such as variations in pH or solvent concentrations. Bacterial membranes play a key role in response to abiotic stresses. Ornithine lipids (OLs) are a group of membrane lipids whose presence and synthesis have been related to stress resistance in bacteria. We wondered if this stress resistance could be transferred to bacteria not encoding the capacity to form OLs in their genome, such as E. coli. In this study, we engineered different E. coli strains to produce unmodified OLs and hydroxylated OLs by expressing the synthetic operon olsFC. Our results showed that OL formation improved pH resistance and increased biomass under phosphate limitation. Transcriptome analysis revealed that OL-forming strains differentially expressed stress- and membrane-related genes. OL-producing strains also showed better growth in the presence of the ionophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP), suggesting reduced proton leakiness in OL-producing strains. Furthermore, our engineered strains showed improved heterologous violacein production at phosphate limitation and also at low pH. Overall, this study demonstrates the potential of engineering the E. coli membrane composition for constructing robust hosts with an increased abiotic stress resistance for biotechnology and synthetic biology applications. Key points • Ornithine lipid production in E. coli increases biomass yield under phosphate limitation. • Engineered strains show an enhanced production phenotype under low pH stress. • Transcriptome analysis and CCCP experiments revealed reduced proton leakage.

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“…However, most of the known routes have only been validated at small volumes. Therefore, in order to achieve industrial levels, it would be necessary to ensure that their bioproduction processes are still efficient and reproducible in terms of titer, yield, and rate ( 6 , 7 ).…”

Section: Introductionmentioning

confidence: 99%

DetSpace: a web server for engineering detectable pathways for bio-based chemical production

Martín Lázaro,

Marín Bautista,

Carbonell

2024

Nucleic Acids Research

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Tackling climate change challenges requires replacing current chemical industrial processes through the rational and sustainable use of biodiversity resources. To that end, production routes to key bio-based chemicals for the bioeconomy have been identified. However, their production still remains inefficient in terms of titers, rates, and yields; because of the hurdles found when scaling up. In order to make production more efficient, strategies like automated screening and dynamic pathway regulation through biosensors have been applied as part of strain optimization. However, to date, no systematic way exists to design a genetic circuit that is responsive to concentrations of a given target compound. Here, the DetSpace web server provides a set of integrated tools that allows a user to select and design a biological circuit that performs the sensing of a molecule of interest by its enzymatic conversion to a detectable molecule through a transcription factor. In that way, the DetSpace web server allows synthetic biologists to easily design biosensing routes for the dynamic regulation of metabolic pathways in applications ranging from genetic circuits design, screening, production, and bioremediation of bio-based chemicals, to diagnostics and drug delivery.

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Milligrams to kilograms: making microbes work at scale

Cited by 13 publications

References 101 publications

New trends in microbial lipid-based biorefinery for fermentative bioenergy production from lignocellulosic biomass

New trends in microbial lipid-based biorefinery for fermentative bioenergy production from lignocellulosic biomass

Enhancing Escherichia coli abiotic stress resistance through ornithine lipid formation

DetSpace: a web server for engineering detectable pathways for bio-based chemical production

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