Mimicked Periosteum Layer Based on Deposited Particle Silk Fibroin Membrane for Osteogenesis and Guided Bone Regeneration in Alveolar Cleft Surgery: Formation and in Vitro Testing

Moe, Yadanar Mya; Nuntanaranont, Thongchai; Khangkhamano, Matthana; Meesane, Jirut

doi:10.1080/15476278.2021.1991743

Cited by 6 publications

(5 citation statements)

References 52 publications

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“…On the other hand, beta sheet has dense packing via hydrogen bonding, which offers higher mechanical strength and toughness than the others. In the bone repair of an alveolar cleft defect, SF has been prepared into two- and three-dimensional biomaterials that are inserted into the defect area to recover the contour shape of the oral cavity [ 27 ]. Some studies have incorporated SF with other materials, to improve the enhancement of bone formation suitable for an alveolar cleft defect [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Osteoconductive Silk Fibroin Binders for Bone Repair in Alveolar Cleft Palate: Fabrication, Structure, Properties, and In Vitro Testing

Sangkert

Juncheed

Meesane

2022

JFB

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Osteoconductive silk fibroin (SF) binders were fabricated for the bone repair of an alveolar cleft defect. Binders were prefigureared by mixing different ratios of a mixture of random coils and SF aggregation with SF fibrils: 100:0 (SFB100), 75:25 (SFB75), 50:50 (SFB50), 25:75 (SFB25), and 0:100 (SFB0). The gelation, molecular organization, structures, topography, and morphology of the binders were characterized and observed. Their physical, mechanical, and biological properties were tested. The SF binders showed gelation via self-assembly of SF aggregation and fibrillation. SFB75, SFB50, and SFB25 had molecular formation via the amide groups and showed more structural stability than SFB100. The morphology of SFB0 demonstrated the largest pore size. SFB0 showed a lowest hydrophilicity. SFB100 showed the highest SF release. SFB25 had the highest maximum load. SFB50 exhibited the lowest elongation at break. Binders with SF fibrils showed more cell viability and higher cell proliferation, ALP activity, calcium deposition, and protein synthesis than without SF fibrils. Finally, the results were deduced: SFB25 demonstrated suitable performance that is promising for the bone repair of an alveolar cleft defect.

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Section: Introductionmentioning

confidence: 99%

Osteoconductive Silk Fibroin Binders for Bone Repair in Alveolar Cleft Palate: Fabrication, Structure, Properties, and In Vitro Testing

Sangkert

Juncheed

Meesane

2022

JFB

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“…Figure 3B illustrates the FT-IR pattern for SF; the broad absorption band at 3281.12 cm À1 is attributable to OH stretching due to intermolecular and intramolecular hydrogen bonding. 30 The three vibrational peaks representing the amide I, amide II, and amide III of pure SF protein can be observed in Figure 3B. The peak at 1620.47 cm À1 confirmed the presence of amide I due to C O stretching; the characteristic amide II peak at approximately 1515.51 cm À1 is attributable to secondary N H bending, and thus confirms the existence of a β-sheet structure in the SF.…”

Section: Ft-ir Analysismentioning

confidence: 69%

“…The broad peak at 1751.44 cm −1 is attributable to CO stretching vibration, and the peaks corresponding to CH 3 , CO bending vibration, and CO stretching vibration 28,29 were respectively observed at 1453.20, 1181.69, and 1084.63 cm −1 . Figure 3B illustrates the FT‐IR pattern for SF; the broad absorption band at 3281.12 cm −1 is attributable to OH stretching due to intermolecular and intramolecular hydrogen bonding 30 . The three vibrational peaks representing the amide I, amide II, and amide III of pure SF protein can be observed in Figure 3B.…”

Section: Resultsmentioning

confidence: 98%

Polylactic acid/silk fibroin composite hollow fibers as excellent controlled drug release systems

Gang

Wang

et al. 2022

Polymers for Advanced Techs

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Polylactic acid (PLA) and silk fibroin (SF) have been widely used in biomedical applications because of their excellent biocompatibility and degradability. In this study, PLA and SF were used as raw materials to prepare hollow fibers with a skin‐core structure by wet spinning technology. Scanning electron microscopy observations revealed that the structure of hollow fibers became increasingly uniform with increasing silk fibroin mass fraction. Tensile test results showed that with the increase of silk fibroin content, the elastic modulus of hollow fibers decreased and their tensile properties improved. The results of hollow fibers degradation experiments revealed that increasing the content of silk fibroin can effectively shorten the degradation time of hollow fibers. Ultraviolet spectrophotometry was used to measure the absorbance of tetracycline hydrochloride in phosphate buffer saline and calculate its release rate in hollow fibers with different silk fibroin contents, the result is HFs‐9 > HFs‐7 > HFs‐0 > HFs‐5 > HFs‐3. The PLA/SF controlled drug release system has precise controlled release of the drug, realizes the separation of the drug from the controlled release system, and solves the problem of sudden drug release. In addition, the controlled release system is non‐toxic, degradable, and has excellent mechanical properties.

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“…Figure 2 c shows that the breaking strengths were ordered RSF/RSS 2 film (3.56 ± 0.37 MPa) > RSF film (3.24 ± 0.39 MPa) > RSF C film (2.60 ± 0.13 MPa). The breaking strengths of RSF/RSS 2 and RSF films were equivalent to natural cornea tissue (approximately 3–5 MPa) 31 , and higher than that of RSF-based biomimetic periosteum prepared by Na 2 CO 3 degumming 32 . Therefore, RSF film and RSF/RSS 2 composite film were considered alternative hard-tissue repair materials such as dura or periosteum.…”

Section: Resultsmentioning

confidence: 88%

Effect of degumming degree on the structure and tensile properties of RSF/RSS composite films prepared by one-step extraction

Tian

et al. 2023

Sci Rep

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Regenerated silk fibroin (RSF) and regenerated sericin (RSS) have attracted much attention for tissue engineering due to excellent biocompatibility and controllable degradation. However, pure RSF films prepared by existing methods are brittle, which limits applications in the field of high-strength and/or flexible tissues (e.g. cornea, periosteum and dura). A series of RSF/RSS composite films were developed from solutions prepared by dissolving silks with different degumming rates. The molecular conformation, crystalline structure and tensile properties of the films and the effect of sericin content on the structure and properties were investigated. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction results revealed more β-sheets in films prepared by boiling water degumming than in Na2CO3-degummed RSFC film. Analysis of mechanical properties showed that the breaking strength (3.56 MPa) and elongation (50.51%) of boiling water-degummed RSF/RSS film were significantly increased compared with RSFC film (2.60 MPa and 32.31%), and the flexibility of films could be further improved by appropriately reducing the degumming rate.

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Mimicked Periosteum Layer Based on Deposited Particle Silk Fibroin Membrane for Osteogenesis and Guided Bone Regeneration in Alveolar Cleft Surgery: Formation and in Vitro Testing

Cited by 6 publications

References 52 publications

Osteoconductive Silk Fibroin Binders for Bone Repair in Alveolar Cleft Palate: Fabrication, Structure, Properties, and In Vitro Testing

Osteoconductive Silk Fibroin Binders for Bone Repair in Alveolar Cleft Palate: Fabrication, Structure, Properties, and In Vitro Testing

Polylactic acid/silk fibroin composite hollow fibers as excellent controlled drug release systems

Effect of degumming degree on the structure and tensile properties of RSF/RSS composite films prepared by one-step extraction

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