This goal of this chapter is to build a bridge between psychophysiology and organizational behavior in an effort to extend organizational theories and enhance the precision of organizational research. The first section describes psychophysiological systems and theories that can inform organizational scholars' understanding of the biological bases of behavior in organizations. The second section discusses the advantages and challenges associated with incorporating psychophysiological measures into organizational research. The third section speculates on some possible domains where the use of psychophysiological measures may provide novel insights into key organizational phenomena. In sum, this chapter provides an opportunity for organizational scholars to think about how incorporating physiological measures and outcomes into organizational research can deepen theoretical insights and enrich our understanding of human behavior in organizations.